A5075755686- Advanced Fluorescence Microscopy Techniques
- Near-Field Optical Microscopy
- Integrated Circuits and Semiconductor Failure Analysis
- Force Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- Atmospheric chemistry and aerosols
- Optical Coherence Tomography Applications
- Flood Risk Assessment and Management
- Atmospheric and Environmental Gas Dynamics
- Air Quality and Health Impacts
- Photoacoustic and Ultrasonic Imaging
- Vehicle emissions and performance
- Air Quality Monitoring and Forecasting
- Indoor and Outdoor Localization Technologies
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Immunotherapy and Immune Responses
- Biosensors and Analytical Detection
- Ionosphere and magnetosphere dynamics
- Water Systems and Optimization
- Optical Imaging and Spectroscopy Techniques
- T-cell and B-cell Immunology
- Advanced biosensing and bioanalysis techniques
- Photoreceptor and optogenetics research
- Ocular Oncology and Treatments
City University of New York
2019-2024
The Graduate Center, CUNY
2019-2024
CUNY Advanced Science Research Center
2019-2024
Memorial Sloan Kettering Cancer Center
2005-2019
Kettering University
2008-2011
Rochester Institute of Technology
1992-1996
North Carolina State University
1996
Optical Sciences (United States)
1994
We present the design and describe operation of a scanning probe microscope which simultaneously provides attractive mode force near-field optical images objects. In this technique, signal is used to track topography, thus allowing primarily show variations in transmissivity. A number results are presented on application technique imaging different samples.
'Molecular signatures' are the qualitative and quantitative patterns of groups biomolecules (e.g., mRNA, proteins, peptides, or metabolites) in a cell, tissue, biological fluid, an entire organism. To apply this concept to biomarker discovery, measurements should ideally be noninvasive performed single read-out. We have therefore developed peptidomics platform that couples magnetics-based, automated solid-phase extraction small peptides with high-resolution MALDI-TOF mass spectrometric...
Regulatory T cells (Tregs) suppress antitumor immunity by inhibiting the killing of tumor antigen-specific CD8+ cells. To better understand mechanisms involved, we used ex vivo three-dimensional collagen-fibrin gel cultures dissociated B16 melanoma tumors. This system recapitulated in suppression antimelanoma immunity, rendering resistant to cocultured activated, Immunosuppression was not observed when tumors excised from Treg-depleted mice were cultured this system. Experiments with...
Abstract Recently, surface-enhanced Raman scattering nanoprobes have shown tremendous potential in oncological imaging owing to the high sensitivity and specificity of their fingerprint-like spectra. As current scanners rely on a slow, point-by-point spectrum acquisition, there is an unmet need for faster cover clinically relevant area real-time. Herein, we report rational design optimization fluorescence-Raman bimodal nanoparticles (FRNPs) that synergistically combine spectroscopy with...
Air quality policies have made substantial gains by reducing pollutant emissions from the transportation sector. In March 2020, New York City's activities were severely curtailed in response to COVID-19 pandemic, resulting 60–90% reductions human activity. We continuously measured major volatile organic compounds (VOCs) during January–April 2020 and 2021 Manhattan. Concentrations of many VOCs decreased significantly shutdown with variations daily patterns reflective activity perturbations, a...
Confocal mosaicing microscopy is a developing technology platform for imaging tumor margins directly in freshly excised tissue, without the processing required conventional pathology. Previously, on 12-×-12 mm 2 of skin tissue from Mohs surgery and detection basal cell carcinoma was demonstrated 9 min. Last year, we reported feasibility faster approach called "strip mosaicing," which 10-×-10 3 Here describe further advances instrumentation, software, speed. A mechanism also developed to...
Cancers of the gastrointestinal (GI) tract are among most frequent and lethal cancers worldwide. An important reason for this high mortality is that early disease typically asymptomatic, patients often present with advanced, incurable disease. Even in high-risk who routinely undergo endoscopic screening, lesions can be missed due to their small size or subtle appearance. Thus, current imaging approaches lack sensitivity specificity accurately detect incipient GI cancers. Here we report our...
Abstract Flooding is one of the most dangerous and costly natural hazards, has a large impact on infrastructure, mobility, public health, safety. Despite disruptive impacts flooding predictions increased due to climate change, municipalities have little quantitative data available occurrence, frequency, or extent urban floods. To address this, we been designing, building, deploying low‐cost, ultrasonic sensors systematically collect presence, depth, duration street‐level floods in New York...
Biological membranes decorated with suitable contrast agents give rise to nonlinear optical signals such as two-photon fluorescence and harmonic up-conversion when illuminated ultra-short, high-intensity pulses of infrared laser light. Microscopic images based on these contrasts were acquired at video or higher frame rates by scanning a focused illuminating spot rapidly across neural tissues. The scan engine relied an acousto-optic deflector (AOD) produce fast horizontal raster corrective...
Imaging large areas of tissue rapidly and with high resolution may enable rapid pathology at the bedside. The limited field view high-resolution microscopes requires merging multiple images that are taken sequentially to cover a area. This or mosaicing long acquisition processing times, produces artifacts. To reduce both time artifacts, we developed method on confocal microscope morphology in excised sub-cellular detail. By acquiring image strips aspect ratios 10:1 higher (instead standard...
Volatile chemical products (VCPs) and other non-combustion-related sources have become important for urban air quality, bottom-up calculations report emissions of a variety functionalized compounds that remain understudied uncertain in estimates. Using new instrumental configuration, we present online measurements oxygenated organic U.S. megacity over 10-day wintertime sampling period, when biogenic photochemistry were less active. Measurements conducted at rooftop observatory upper...
Abstract. Accurately quantifying methane emissions from cities and understanding the processes that drive them are important for reaching climate mitigation goals. Methane New York City metropolitan area (NYCMA), most populous urban of United States, have consistently been underestimated by emission inventories compared to aircraft satellite observations. In this study, we used continuous rooftop measurements over 6 winter-to-spring transitions (January–May, 2019–2024) examine variability...
Pseudoheterodyne detection of sample light in near field optical microscopy is demonstrated an actively stabilized Mach–Zehnder interferometer. This results a significant signal enhancement. Three distinct imaging modalities are described, which based on the various stabilization feedback parameters, and scan speeds. A number images presented, demonstrating both amplitude phase contrast modalities.
Minimally invasive surgical (MIS) techniques, such as laparoscopic surgery and endoscopy, provide reliable disease control with reduced impact on the function of diseased organ. Surgical lasers can ablate, cut excise tissue while sealing small blood vessels minimizing bleeding risk lymphatic metastases from tumors. Lasers wavelengths in IR are readily absorbed by water causing minimal thermal damage to adjacent tissue, ideal for near critical anatomical structures. MIS techniques have...
Variation of the reflectance aluminized tip a near-field scanning optical microscope is used to measure temperature rise due confinement light in tip. The measurement technique involves pump-probe beam approach, and uses two-step process which eliminates need know dependence signal on scattering cross section
The design and theory of operation a new form near field polarizing optical microscope are presented. system uses electro-optic premodulation light to generate two simultaneous complementary images samples. This affords the capability obtain final output signal which is linear representation sample birefringence, independent transmissivity/reflectivity. A number
Determining how tumor immunity is regulated requires understanding the extent to which anti-tumor immune response "functions" in vivo without therapeutic intervention. To better understand this question, we developed advanced multimodal reflectance confocal/two photon fluorescence intra-vital imaging techniques use combination with traditional ex analysis of specific T cells. By transferring small numbers melanoma-specific CD8+ cells (Pmel-1), an attempt mimic physiologic conditions, found...
Operational aspects of a newly developed simultaneous near-field optical and force microscope are considered. Using glass probe, the technique is capable monitoring sample topography, through its microscopy capability, as well sample’s parameters, using light guiding properties tip. In addition, signal can be used reliably feedback signal, thus allowing crash-free detection fields in vicinity opaque steps on sample. Results presented imaging variety samples including biological nonbiological...
Birefringence, arising from locally preferred orientation of molecules or functional groups in polymeric and related materials has been observed a near-field scanning optical microscope (NSOM) at suboptical spatial resolution. Our observations were successfully correlated to existing structural models the samples, demonstrating utility birefringence NSOM. We present data acquired thin sections partially ordered polymer fiber (Kevlar) dispersed small-molecule-liquid-crystal composite.