A5016253739- Advanced Radiotherapy Techniques
- Radiation Therapy and Dosimetry
- Advanced biosensing and bioanalysis techniques
- Lung Cancer Diagnosis and Treatment
- Nanoparticle-Based Drug Delivery
- Medical Imaging Techniques and Applications
- Nanoplatforms for cancer theranostics
- Click Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Radiopharmaceutical Chemistry and Applications
- Head and Neck Cancer Studies
- Radiation Dose and Imaging
- Nuclear Physics and Applications
- Advanced X-ray and CT Imaging
- RNA Interference and Gene Delivery
- Plant Genetic and Mutation Studies
- Boron Compounds in Chemistry
- Electrical and Bioimpedance Tomography
- Advanced Optical Sensing Technologies
- Esophageal Cancer Research and Treatment
- Microplastics and Plastic Pollution
- Virus-based gene therapy research
- Neuroendocrine Tumor Research Advances
- Magnetic Field Sensors Techniques
- Supramolecular Self-Assembly in Materials
Technical University of Denmark
2010-2020
Theranostics (New Zealand)
2014-2020
Ørsted (Denmark)
2014-2018
Clinical applications of siRNA for treating disorders in the central nervous system require development systemic stable, safe, and effective delivery vehicles that are able to cross impermeable blood-brain barrier (BBB). Engineering nanocarriers with low cellular interaction during circulation, but high uptake targeted cells, is a great challenge further complicated by BBB. As first step obtaining such system, this study aims at designing lipid nanoparticle (LNP) efficiently encapsulate...
Liposomal oxaliplatin remodels the tumor microenvironment and potentiate TLR-agonist–mediated immune responses in cancer.
A major challenge in nanoecotoxicology is finding suitable methods to determine the uptake and localisation of nanoparticles on a whole-organism level. Some have been associated with artefacts induced by sample preparation, including staining for electron microscopy. This study used light sheet microscopy (LSM) define fluorescently labelled living organisms minimal preparation. Zebrafish (Danio rerio) were exposed fluorescent gold (Au NPs) polystyrene NPs via aqueous or dietary exposure. The...
By taking advantage of the ability 64Cu to bind nonspecifically gold surfaces, we have developed a methodology embed this radionuclide inside nanoparticles (AuNPs). enables in vivo imaging AuNPs by positron emission tomography (PET). multitude uses within health technology and are useful tools for general nanoparticle research. 64Cu-AuNPs were prepared incubating AuNP seeds with 64Cu2+, followed entrapment grafting on second layer gold. This resulted radiolabeling efficiencies 53 ± 6%. The...
We present here a highly efficient and chemoselective liposome functionalization method based on oxime bond formation between hydroxylamine an aldehyde-modified lipid component. have conducted systematic quantitative comparison of this new approach with other state-of-the-art conjugation reactions in the field. Targeted liposomes that recognize overexpressed receptors or antigens diseased cells great potential therapeutic diagnostic applications. However, chemical modifications nanoparticle...
A new biodegradable liquid fiducial marker was devised to allow for easy insertion in lung tumors using thin needles. The purpose of this study evaluate the visibility markers image-guided radiation therapy and compare existing solid one currently commercially available.Fiducial quantified terms contrast noise ratio (CNR) on planar kilovoltage x-ray images a thorax phantom different concentrations radio-opaque component marker, four markers, marker. Additionally, image artifacts produced...
In the western world, approximately 50% of all cancer patients receive radiotherapy alone or in combination with surgery chemotherapy. Image‐guided (IGRT) has recent years been introduced to enhance precision delivery radiation dose tumor tissue. Fiducial markers are often inserted inside improve IGRT and enable monitoring position during therapy. present article, a liquid fiducial tissue marker is presented, which can be injected into using thin flexible needles. The high radio‐opacity,...
Background and Objective: Hepatocellular carcinoma (HCC) is the most common primary liver malignancy, its current management relies heavily on locoregional therapy for curative therapy, bridge to transplant, palliative therapy.Locoregional therapies include ablation hepatic artery directed such as embolization radioembolization.In this study we evaluate feasibility of using novel antivascular ultrasound (AVUS) a noninvasive reduce perfusion in HCC lesions rat model monitor effect with...
External beam radiation therapy relies on the ability to deliver high doses tumor cells with minimal exposure surrounding healthy tissue. Advanced irradiation techniques, including image‐guided (IGRT), rely locate tumors optimize therapeutic benefit of these techniques. Today, radiopaque fiducial tissue markers are placed in or around tumors, for example, prostate cancer patients enhance precision daily and/or real‐time IGRT. A liquid injectable marker (nanogel) is developed based PEGylated...
X-ray-based position verification of the target volume in image-guided radiation therapy (IGRT) patients with pancreatic ductal adenocarcinoma (PDAC) is currently performed on solid fiducial markers that are implanted under endoscopic ultrasonography. A new biodegradable liquid marker has recently been introduced. To assess its potential use for magnetic resonance imaging (MRI)-guided photon or proton radiotherapy PDAC, MRI visibility and artifacts this were quantified compared against gold...
A versatile methodology for efficient synthesis of PEGylated lipopeptides via CuAAC "Click" conjugation between alkyne-bearing solid-supported and azido-functionalized PEGs is described. This new very robust method offers a unique platform synthesizing with high level complexity. These molecules, obtained in single purification step, are ideally suited functionalization lipid bilayers liposomal drug delivery systems particularly valuable enzyme activation strategies.
Gene carriers made from synthetic materials are of interest in relation to gene therapy but suffer lack transfection efficiency upon systemic delivery. To address this problem, a novel lipo‐peptide‐PEG conjugate constituted by lipid‐anchor, peptide sensitive proteases and poly (ethylene glycol) (PEG) chain is investigated. Utilizing ethanol‐mediated nucleic acid encapsulation prepare lipo‐nanoparticles (LNPs), LNPs that stable serum obtained. The constitute highly effective delivery systems...
Safety and clinical feasibility of injecting a novel liquid fiducial marker for use in image guided radiotherapy 15 patients with non-small cell lung cancer are reported. No major safety or toxicity issues were encountered. Markers present at start remained visible cone beam computed tomography fluoroscopy images throughout the treatment course on during follow-up (0–38 months). Marker volume reduction was seen until 9 months after treatment, which no further breakdown found. post-treatment...
To evaluate the feasibility of a new liquid fiducial marker for use in image-guided radiotherapy oesophageal cancer.Liquid markers were implanted patients with metastatic or inoperable locally advanced gastro-oesophageal junction cancer receiving radiotherapy. Markers using conventional gastroscope equipped 22 G Wang needle. Marker visibility was evaluated on fluoroscopy, CT, MRI and cone beam CT scans.Liquid (n = 16) injected four patients. No Grade 2 worse adverse events observed relation...
The liquid-based Carbo-gel technology enables real-time multimodal image guidance for improved therapeutic precision.
Functionalization of nanoparticles is a key element for improving specificity drug delivery systems toward diseased tissue or cells. In the current study we report highly efficient and chemoselective method post-functionalization liposomes with biomacromolecules, which equally well can be used functionalization other solid surfaces. The exploits synergistic effect having both affinity covalent anchoring tags on surface liposome. This was achieved by synthesizing peptide linker system that...
A highly efficient method for functionalizing nanoparticles and directly quantifying conjugation efficiency ligand surface density has been developed. Attachment of 3-azido-modifed RGD-peptides to PEGylated liposomes was achieved by using Cu-free click conditions. Upon coupling a fluorophore is formed, which could be utilized monitor the obtained in situ, without prior purification. Detailed facts importance specialist readers are published as ”Supporting Information”. Such documents...
Development of safe and efficient radiotherapy routines requires quantification the delivered absorbed dose to cancer tissue in individual patients. In vivo dosimetry can provide accurate information about during treatment. current study, a novel silver-nanosensor formulation based on poly(vinylpyrrolidinone)-coated silver nanoparticles formulated gelation matrix composed sucrose acetate isobutyrate has been developed for use as an dosimeter external beam radiotherapy. situ photonuclear...
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