A5077145110- Bacterial biofilms and quorum sensing
- Microbial Inactivation Methods
- Bacterial Genetics and Biotechnology
- Ultrasound and Cavitation Phenomena
- Lipid Membrane Structure and Behavior
- Bacteriophages and microbial interactions
- Surfactants and Colloidal Systems
- Field-Flow Fractionation Techniques
- Membrane Separation Technologies
- Probiotics and Fermented Foods
- Antimicrobial Peptides and Activities
- Spectroscopy and Quantum Chemical Studies
- Pickering emulsions and particle stabilization
- Biofield Effects and Biophysics
- RNA and protein synthesis mechanisms
- Microfluidic and Bio-sensing Technologies
- Aerosol Filtration and Electrostatic Precipitation
- Cavitation Phenomena in Pumps
University of Ljubljana
2019-2023
Liposomes are widely applied in research, diagnostics, medicine and industry. In this study we show for the first time effect of hydrodynamic cavitation on liposome stability compare it to well described chemical, physical mechanical treatments. Fluorescein loaded giant 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipid vesicles were treated with as promising method inactivation biological samples. Hydrodynamic treatment was compared various stressors such ionic strength osmolarity agents...
Cavitation is a potentially useful phenomenon accompanied by extreme conditions, which one of the reasons for its increased use in variety applications, such as surface cleaning, enhanced chemistry, and water treatment. Yet, we are still not able to answer many fundamental questions related efficacy effectiveness cavitation treatment, as: "Can single bubbles destroy contaminants?" "What precisely mechanism behind bubble's cleaning power?". For these reasons, present paper addresses tool...
Anaerobic digestion in wastewater treatment plants converts its unwanted end product – waste activated sludge into biogas. Even if the process is well established, pre-treatment of can further improve efficiency. In this study, four regimes for increasing methane production through prior disintegration were investigated using lab-scale cavitation generator and real samples. Three different cavitating (attached regime, developed cloud shedding regime a wake regime) one non-cavitating at...
Surfactin uniquely influences lipid bilayer structure by initially inducing membrane invaginations before solubilization. In this study, we exposed DOPC giant vesicles to various surfactin concentrations at different temperatures and observed surfactin-induced using differential interference contrast confocal laser fluorescence microscopy. These were stable room temperature but not higher temperatures. molecules induce nanodomains with negative spontaneous curvature despite their intrinsic...
The applications of bacterial sonolysis in industrial settings are plagued by the lack knowledge exact mechanism action sonication on cells, variable effectiveness cavitation bacteria, and inconsistent data its efficiency. In this study we have systematically changed material properties E. coli cells to probe effect different cell wall layers resistance ultrasonic irradiation (20 kHz, output power 6,73 W, horn type, 3 mm tip diameter, 1 ml sample volume). We determined rates decay for...
Abstract Bacterial quorum sensing (QS) is based on signal molecules (SM), which increase in concentration with cell density. At critical SM concentration, a variety of adaptive genes sharply change their expression from basic level to maximum level. In general, this sharp transition, hallmark true QS, requires an dependent positive feedback loop, where enhances its own production. Some communication systems, like the peptide SM-based ComQXPA system Bacillus subtilis , do not have loop and we...
The applications of bacterial sonolysis in industrial settings are plagued by the lack knowledge exact mechanism action sonication on cells, variable effectiveness cavitation bacteria, and inconsistent data its efficiency. In this study we have systematically changed material properties E. coli cells to probe effect different cell wall layers resistance sonolysis. We determined rates decay for bacteria with compromised major capsular polymers, disrupted outer membrane, peptidoglycan layer,...