A5063938952- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Microbial Community Ecology and Physiology
- Advances in Cucurbitaceae Research
- Plant Physiology and Cultivation Studies
- Rice Cultivation and Yield Improvement
- Microbial infections and disease research
- Photosynthetic Processes and Mechanisms
- GABA and Rice Research
- Genetics and Plant Breeding
- Cell Image Analysis Techniques
- Micro and Nano Robotics
- Free Radicals and Antioxidants
- Agricultural Science and Fertilization
- Plant Molecular Biology Research
- Sentiment Analysis and Opinion Mining
- Genomics, phytochemicals, and oxidative stress
- Plant Pathogens and Fungal Diseases
- Plant tissue culture and regeneration
- Legionella and Acanthamoeba research
- Banana Cultivation and Research
- Genomics and Phylogenetic Studies
- Biocrusts and Microbial Ecology
Indian Agricultural Research Institute
2002-2025
University of Miami
2025
All India Institute of Medical Sciences Raipur
2024
All India Institute of Medical Sciences Bhopal
2020-2024
All India Institute of Medical Sciences Rishikesh
2022-2024
Indian Council of Agricultural Research
2024
National Bureau of Plant Genetic Resources
2024
Graphic Era University
2023
ICAR - Central Island Agricultural Research Institute
2018-2023
Bharati Vidyapeeth Deemed University
2022-2023
Abstract Biofilms are microbial communities that represent a highly abundant form of life on Earth. Inside biofilms, phenotypic and genotypic variations occur in three-dimensional space time; microscopy quantitative image analysis therefore crucial for elucidating their functions. Here, we present BiofilmQ—a comprehensive cytometry software tool the automated high-throughput quantification, visualization numerous biofilm-internal whole-biofilm properties time.
Significance Most living systems, from individual cells to tissues and swarms, display collective self-organization on length scales that are much larger than those of the units drive this organization. A fundamental challenge is understand how properties microscopic components determine macroscopic, multicellular biological function. Our study connects intracellular physiology macroscale behaviors during development, spanning five orders magnitude in six time, using bacterial swarming as a...
Bacteria can generate benefits for themselves and their kin by living in multicellular, matrix-enclosed communities, termed biofilms, which are fundamental to microbial ecology the impact bacteria have on environment, infections, industry [1-6]. The advantages of biofilm mode life include increased stress resistance access concentrated nutrient sources [3, 7, 8]. However, there also costs associated with growth, including metabolic burden matrix production, resource competition, limited...
Horizontal gene transfer mediated by plasmid conjugation plays a significant role in the evolution of bacterial species, as well dissemination antibiotic resistance and pathogenicity determinants. Characterization their regulation is important for gaining insights into these features. Relatively little known about how Gram-positive plasmids regulated. We have characterized native Bacillus subtilis pLS20. Contrary to enterococcal plasmids, pLS20 not activated recipient-produced pheromones but...
Biofilm formation is critical for the infection cycle of Vibrio cholerae. exopolysaccharides (VPS) and matrix proteins RbmA, Bap1 RbmC are required development biofilm architecture. We demonstrate that RbmA binds VPS directly uses a binary structural switch within its first fibronectin type III (FnIII-1) domain to control dynamics VPS-dependent higher-order structures. The in FnIII-1 regulates interactions trans with FnIII-2 domain, leading open (monomeric) or closed (dimeric) interfaces....
Bacterial biofilms represent a major form of microbial life on Earth and serve as model active nematic system, in which activity results from growth the rod-shaped bacterial cells. In their natural environments, ranging human organs to industrial pipelines, have evolved grow robustly under significant fluid shear. Despite intense practical theoretical interest, it is unclear how strong flow alters local global architectures biofilms. Here, we combine highly time-resolved single-cell live...
Bacteriophages can be trapped in the matrix of bacterial biofilms, such that cells inside them are protected. It is not known whether these phages still infectious and they pose a threat to newly arriving bacteria. Here, we address questions using Escherichia coli its lytic phage T7. Prior work has demonstrated T7 bound outermost curli polymer layers E. biofilm matrix. We show do remain viable kill colonizing T7-susceptible. If colonize resident before do, find killed by exposure if it...
Objectives The spread of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus has been unprecedentedly fast, spreading to more than 180 countries within 3 months with variable severity. One the major reasons attributed this variation is genetic mutation. Therefore, we aimed predict mutations in spike protein (S) SARS-CoV-2 genomes available worldwide and analyze its impact on antigenicity. Materials Methods Several research groups have generated whole genome sequencing data...
Under certain growth conditions, Bacillus subtilis can develop natural competence, the state in which it is able to bind, adsorb and incorporate exogenous DNA. Development of competence a bistable process subject complex regulation. Rok repressor key transcriptional activator genes, comK, limits size subpopulation that develops competence. Here we report finding large conjugative B. plasmid pLS20 harbours rok homologue rok(LS20). Although deduced product rok(LS20) considerably shorter than...
Antibiotic resistance is a serious global problem. genes (ARG), which are widespread in environmental bacteria, can be transferred to pathogenic bacteria via horizontal gene transfer (HGT). Gut microbiomes especially apt for the emergence and dissemination of ARG. Conjugation HGT route that predominantly responsible spread Little known about conjugative elements Gram-positive including those phylum Firmicutes, abundantly present gut microbiomes. A critical step conjugation process...
Drought stress tolerance for crop improvement is an important goal worldwide. a complex trait, and it vital to understand the physiological, biochemical, molecular mechanisms of drought tackle effectively. Osmotic adjustment, oxidative management (OSM), cell membrane stability (CMS) are major components cellular under stress. In current study, we explored basis OSM in tolerant rice variety, Nagina 22 compared with popular sensitive IR 64, imposed at reproductive stage, how parental...
Abstract Vibrio cholerae , the cause of cholera disease, exhibits a characteristic curved rod morphology, which promotes infectivity and motility in dense hydrogels. Periplasmic protein CrvA determines cell curvature V. yet regulatory factors controlling are unknown. Here, we discover VadR small RNA (sRNA) as post-transcriptional inhibitor crvA mRNA. Mutation vadR increases curvature, whereas overexpression has inverse effect. We show that transcription is activated by VxrAB two-component...
A field experiment was conducted during the Kharif seasons of 2022 and 2023 at Students' Instructional Farm, Acharya Narendra Deva University Agriculture Technology, Kumarganj Ayodhya, to assess impact graded doses biochar, varying fertility levels, addition biofertilizers zinc on rice cultivation under partially reclaimed sodic soils. Nine treatments were applied, including combinations recommended fertilizers (RDF), biochar (5 t ha⁻¹ 2.5 ha⁻¹), biofertilizer (ZMB), sulphate (ZnSO₄) in a...
The occurrence of subretinal hemorrhage due to a peripapillary choroidal neovascular membrane (CNVM) is rare complication idiopathic intracranial hypertension (IIH). We report case 32-year-old obese female who presented with headache associated profound loss vision in the left eye for 2 weeks. After an ocular examination, she was diagnosed IIH, which had led formation CNVM eye. She advised make lifestyle modifications and lose weight started on oral acetazolamide. Antivascular endothelial...
Abstract Astrocytes participate in synaptic transmission and plasticity through tightly regulated, bidirectional communication with pre- post-synaptic neurons, as well microglia oligodendrocytes. A key component of astrocyte-mediated regulation is the cytokine tumor necrosis factor (TNF). TNF signals via two cognate receptors, TNFR1 TNFR2, both expressed astrocytes. While signaling astrocytes has been long demonstrated to be necessary for physiological function, role astroglial TNFR2 never...
ABSTRACT A Candida albicans mutant with mutations in the N -acetylglucosamine (GlcNAc) catabolic pathway gene cluster, including GlcNAc-6-phosphate deacetylase ( DAC1 ), glucosamine-6-phosphate deaminase NAG1 and GlcNAc kinase HXK1 ) genes, was not able to grow on amino sugars, exhibited highly attenuated virulence a murine systemic candidiasis model, less adherent human buccal epithelial cells vitro. No germ tubes were formed by after induction GlcNAc, but hyperfilamentation under...
Abstract Biofilms are now considered to be the most abundant form of microbial life on Earth, playing critical roles in biogeochemical cycles, agriculture, and health care. Phenotypic genotypic variations biofilms generally occur three-dimensional space time, therefore often investigated using microscopy. However, quantitative analysis microscopy images presents a key obstacle phenotyping biofilm communities single-cell heterogeneity inside biofilms. Here, we present BiofilmQ, comprehensive...