A5010408758- Immune Cell Function and Interaction
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Liver Disease Diagnosis and Treatment
- Natural Compounds in Disease Treatment
- Cardiovascular Health and Risk Factors
- RNA and protein synthesis mechanisms
- Lipid metabolism and disorders
- Cancer Immunotherapy and Biomarkers
- Circular RNAs in diseases
- Single-cell and spatial transcriptomics
- Traditional Chinese Medicine Studies
- Genomics and Phylogenetic Studies
- Medical Imaging and Pathology Studies
- Soft tissue tumor case studies
- Protein Structure and Dynamics
- RNA modifications and cancer
- Foot and Ankle Surgery
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Sarcoidosis and Beryllium Toxicity Research
- Intestinal and Peritoneal Adhesions
- Bone Metabolism and Diseases
- Appendicitis Diagnosis and Management
- Protein Hydrolysis and Bioactive Peptides
Shanghai Pudong New Area Gongli Hospital
2021-2025
Second Military Medical University
2021-2025
Technical University of Munich
2019-2025
Jiangsu Provincial Center for Disease Control and Prevention
2024
The University of Texas Health Science Center at Houston
2024
Jiujiang First People's Hospital
2023
Ningxia Medical University
2019-2022
China Agricultural University
2022
Third Affiliated Hospital of Inner Mongolia Medical College
2018
Inner Mongolia Medical University
2018
RNA-seq by poly(A) selection is currently the most common protocol for whole transcriptome sequencing as it provides a broad, detailed, and accurate view of RNA landscape. Unfortunately, utility libraries greatly limited when input degraded, which norm research tissues clinical samples, especially specimens are formalin-fixed. To facilitate use beyond cell lines in setting, we developed an exome-capture with improved performance on degraded RNA. Capture enable measuring absolute differential...
T cell maintenance in chronic infection and cancer follows a hierarchical order. Short-lived effector CD8 cells are constitutively replaced from proliferation-competent Tcf1-expressing progenitor population. This occurs spontaneously at low levels increases magnitude upon blocking PD-1 signaling. We explore how CD4 help controls transition survival of the progenitors their progeny by utilizing single-cell RNA sequencing. Unexpectedly, absence caused reductions numbers only among terminally...
Next-generation DNA sequencing has revolutionized the study of biology. However, short read lengths dominant instruments complicate assembly complex genomes and haplotype phasing mixtures similar sequences. Here we demonstrate a method to reconstruct sequences individual nucleic acid molecules up 11.6 kilobases in length from (150-bp) reads. We show that our can construct 99.97%-accurate synthetic reads bacterial, plant, animal genomic samples, full-length mRNA human cancer cell lines, HIV...
Soft tissue defects and postoperative wound healing complications related to calcaneus fractures may result in significant morbidity. The aim of this study was investigate whether percutaneous minimally invasive screw internal fixation (PMISIF) can change situation the treatment calcaneal fractures, aimed explore mechanical effects different methods on Sanders type III through finite element analysis. This retrospective analysis focused 83 patients with II from March 2017 2022. Among them,...
Idiopathic pulmonary fibrosis (IPF) is a genetic heterogeneous disease with high mortality and poor prognosis. However, large fraction of cause remains unexplained, especially in sporadic IPF (∼80% IPF). By systemically reviewing related literature potential pathogenic pathways, 92 potentially IPF-related genes were selected sequenced genomic DNAs from 253 patients 125 matched health controls using targeted massively parallel next-generation sequencing. The identified risk variants confirmed...
Background Type 2 diabetes (T2D) is a common chronic disease with many serious complications. Celastrol can prevent and treat type by reversing insulin resistance in number of ways. However, the specific mechanisms which celastrol prevents treats T2D are not well understood. The aim this study was to explore key gene targets potential signaling pathway for treatment T2D. Methods GSE184050 downloaded from Gene Expression Omnibus online database. Blood samples patients healthy individuals were...
Abstract Single-cell RNA sequencing in principle offers unique opportunities to improve the efficacy of contemporary T-cell based immunotherapy against cancer. The use high-quality single-cell data will aid our incomplete understanding molecular programs determining differentiation and functional heterogeneity cytotoxic T lymphocytes (CTLs), allowing for optimal therapeutic design. So far, a major obstacle high depth analysis CTLs is minute amount available, leading low capturing efficacy....
Abstract Objectives To investigate the diagnostic value of unenhanced CT in mechanical small bowel obstruction (SBO) with necrosis, and to establish a predictive model. Methods From May 2017 December 2021, patients SBO admitted our hospital were retrospectively collected. Taking pathology-confirmed necrosis as gold standard, experimental group was composed confirmed by pathology, control no intestinal surgery or successful conservative treatment recurrence during 1-month followed-up. Results...
Muscle atrophy due to colorectal cancer severely reduces the quality of life and survival time patients. However, underlying causative mechanisms therapeutic agents are not well understood. The aim this study was screen identify microRNA (miRNA)-mRNA regulatory network targets celastrol in causing muscle via blood exosomes. Datasets were downloaded from Gene Expression Omnibus online database. Differential expression analysis first performed using exosome dataset GSE39833 normal humans...
Osteoblast differentiation is a complex process that essential for normal bone formation. A growing number of studies have shown microRNAs (miRNAs) are key regulators in variety physiological and pathological processes, including osteogenesis. In this study, BMP2 was used to induce MC3T3-E1 cells construct osteoblast cell model. Then, we investigated the effect miR-452-3p on related molecular mechanism by RT-PCR analysis, Western blot ALP activity, Alizarin Red Staining. We found...
Abstract Next-generation DNA sequencing has revolutionized the study of biology. However, short read lengths dominant instruments complicate assembly complex genomes and haplotype phasing mixtures similar sequences. Here we demonstrate a method to reconstruct sequences individual nucleic acid molecules up 11.6 kilobases in length from (150-bp) reads. We show that our can construct 99.97%-accurate synthetic reads bacterial, plant, animal genomic samples, full-length mRNA human cancer cell...
<title>Abstract</title> Accurate protein-ligand binding affinity prediction is crucial in drug discovery. Existing methods are predominately docking-free, without explicitly considering atom-level interaction between proteins and ligands scenarios where crystallized conformations unavailable. Now, with breakthroughs deep learning AI-based protein folding conformation prediction, can we improve prediction? This study introduces a framework, Folding-Docking-Affinity (FDA), which folds...
Objective To compare the applicability of three diagnostic criteria for metabolic syndrome (MS) proposed by International Diabetes Federation(IDF) in 2005 ,Adult Treatment Panel Ⅲ National Cholesterol Education Program(NCEP-ATP Ⅲ) and Chinese Socie(CDS) 2004. Methods Based on findings cohort study multiple disorders (1971 cases) Jiangsu province, MS was diagnosed according to these definitions respectively,and calculating sensitivity, specificity, ROC curve distance, those with lower false...
Abstract Summary The Advanced capacities of high throughput single cell technologies have facilitated a great understanding complex biological systems, ranging from heterogeneity to molecular expression kinetics. Several pipelines been introduced standardize the scRNA-seq analysis workflow. These include population identification, marker detection and trajectory reconstruction. Yet, establishing systematized pipeline capture regulatory relationships among transcription factors (TFs) genes at...