A5050544360- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive biology and impacts on aquatic species
- Reproductive Physiology in Livestock
- Wildlife Ecology and Conservation
- Genetic and phenotypic traits in livestock
- Reproductive Health and Technologies
- Effects and risks of endocrine disrupting chemicals
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ovarian function and disorders
- Renal and related cancers
- Selenium in Biological Systems
- Animal Genetics and Reproduction
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Heat shock proteins research
- Effects of Environmental Stressors on Livestock
- Bee Products Chemical Analysis
- Seed Germination and Physiology
- Ecology and biodiversity studies
- Heavy Metal Exposure and Toxicity
- Toxin Mechanisms and Immunotoxins
- Milk Quality and Mastitis in Dairy Cows
- Urinary Bladder and Prostate Research
- Vector-Borne Animal Diseases
Instituto de Investigación en Recursos Cinegéticos
2012-2024
Consejo Superior de Investigaciones Científicas
2013-2024
Hospital General Universitario de Ciudad Real
2022
Regional Government of Castile-La Mancha
2014-2022
University of Castilla-La Mancha
2012-2022
Charles University
2016-2020
Pilsen Tools (Czechia)
2016-2018
Centro Regional de Selección y Reproducción Animal
2008-2012
Instituto de Investigación Sanitaria del Principado de Asturias
2006
Universidad de Oviedo
2006
Fe 2 + /ascorbate, hydrogen peroxide (H O ), and hypoxanthine/xanthine oxidase (XOD) are commonly used for inducing oxidative stress on spermatozoa. A comparative study of these agents was carried out thawed spermatozoa from red deer. First, we tested a high, medium, low concentration each agent: 100, 10, 1 μM (hydroxyl radical generator); mM, 10 H ; mU/ml XOD (superoxide generator), incubated at 37 °C 180 min. Intracellular reactive oxygen species (ROS; DCFDA) increased with dose time...
Sperm design and velocity play key roles in influencing sperm performance and, therefore, can determine fertilization success. Several interspecific studies have demonstrated how these features correlate, it has been hypothesized that selection may drive changes traits. Here, we examine the association between swimming a study conducted at an intraspecific level Iberian red deer (Cervus elaphus hispanicus). We addressed structure of different subpopulations, based on morphometry velocity,...
Sperm cryopreservation has become an indispensable tool in biology. Initially, studies were aimed towards the development of efficient freezing protocols different species that would allow for storage semen samples long periods time, ensuring its viability. Nowadays, it is widely known important individual component exists cryoresistance semen, and efforts are at identifying those sperm characteristics may us to predict this cryoresistance. This knowledge lead, ultimately, design optimized...
The present study evaluated modifications occurring in thawed ram spermatozoa during incubation different media that supported vitro capacitation and fertilisation, examines how these changes relate to IVF. Thawed sperm samples were incubated under capacitating (Cap) non-capacitating (non-Cap) conditions for 0, 1 2 h used an IVF test. During incubation, related membrane status the motility pattern of assessed, latter being characterise subpopulations. A significantly greater increase (P ≤...
The present study addresses the effect of heat stress on males' reproduction ability. For that, we have evaluated sperm DNA fragmentation (DFI) by SCSA ejaculates incubated at 37 °C during 0, 24 and 48 hours after its collection, as a way to mimic temperature circumstances which spermatozoa will be subject in ewe uterus. effects temperature-humidity index (THI) from day 60 prior collection date semen DFI were examined. To better understand causes determining sensitivity heat, this was...
Chromatin remodeling, including histone post-translational modifications, during spermatogenesis can affect sperm quality and fertility, epigenetic marks may therefore be useful for clinical evaluations of sperm. Together with hyperacetylation, the dimethylation H3 on lysine K4 (H3K4me2) is also required protamination. Accordingly, we evaluated utilization this mark identification decrease immature chromatin. In study, 99 semen samples, 22 normozoospermic (N), 63 asthenozoospermic (A), 14...