A5064529599- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive biology and impacts on aquatic species
- Reproductive Physiology in Livestock
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ovarian function and disorders
- Animal Genetics and Reproduction
- Wildlife Ecology and Conservation
- Seed Germination and Physiology
- Comparative Animal Anatomy Studies
- Congenital heart defects research
- Bee Products Chemical Analysis
- Genetic and phenotypic traits in livestock
- Urological Disorders and Treatments
- Reproductive Health and Technologies
- Morphological variations and asymmetry
- Developmental Biology and Gene Regulation
- Chronic Myeloid Leukemia Treatments
- Cell Adhesion Molecules Research
- Sexual Differentiation and Disorders
- Herpesvirus Infections and Treatments
- Plant and fungal interactions
- Genomic variations and chromosomal abnormalities
- Platelet Disorders and Treatments
- Proteoglycans and glycosaminoglycans research
Universidad de León
2016-2025
Bayer (United States)
2015-2016
Biolog (United States)
2015-2016
Hospital Universitario Ramón y Cajal
1992-2001
San Francisco General Hospital
1992
University of California, San Francisco
1992
Cancer Research Institute
1992
Collège de France
1986
Centre National de la Recherche Scientifique
1986
Universidad de León
1985
We have applied a statistical protocol based on principal component analysis, clustering methods, and discriminant analysis for the identification of sperm subpopulations in computer-assisted (CASA) data. Samples were obtained from cauda epididymis 11 Iberian red deer cryopreserved following standard protocol. Motility by CASA was analyzed just after recovery, before freezing, thawing, eight motility descriptors each individual spermatozoon recorded. Sperm viability acrosomal status also...
The reduced lifespan of cryopreserved spermatozoa in the mare reproductive tract has been attributed to both capacitative and apoptotic changes. However, there is a lack studies investigating phenomena simultaneously. In order improve our knowledge this particular point, we studied raw frozen-thawed samples markers using wide battery test based flow cytometry. Apoptotic evaluated were caspase 3 activity, externalization phosphatidylserine (PS), mitochondrial membrane potential. Markers...
We investigated the effects of ethanol exposure on shape cell and morphology intermediate filaments (IF) cortical astrocytes in primary culture. The content distribution glial fibrillary acidic protein (GFAP), major component IF, was assessed using an anti-GFAP monoclonal antibody fluorescence scanning densitometry together with quantitative pre- post-embedding immunogold electron microscopy. were from 21-day-old fetuses obtained both control chronic alcoholic rats cultured for 28 days...
ABSTRACT: The main goal of this study was to investigate the potential protective effects enzymatic and nonenzymatic antioxidants on cryopreservation injuries red deer epididymal spermatozoa. In Experiment 1, sperm freezability catalase, superoxide dismutase, a combination thereof were studied. 2, cryoresistance evaluated when different antioxidants, such as vitamin E, C, butylated hydroxytoluene (BHT), added freezing extender. Sperm quality judged in vitro by microscopic assessments...
To improve artificial insemination protocols in ovine species it is crucial to optimize sperm quality evaluation after preservation technologies. Emerging technologies based on novel biomolecules and related redox balance proteins involved motility such as ProAKAP4 could be successfully applied ram evaluation. In this work, a multiparametric analysis of fresh, cooled, cryopreserved was performed at different complexity levels. Samples were evaluated terms (total motility, progressive...
The morphological characteristics of bovine and equine gustatory lingual papillae are compared by scanning electron microscopy. fungiform in the cow have a shape that corresponds to their name, while horse, they almost do not emerge from surface tongue. These show taste pores both species. vallate papillae, four times larger horse than cow, complex organization secondary grooves horse. In occur single surrounded thick annular pad mucosa. Taste been observed species, whereas foliate present...
Summary The aim of this study was to find the relationship between fertility (as 90‐day non‐return rates) and DNA fragmentation assessed by two techniques [sperm chromatin structure assay (SCSA) Sperm–Bos–Halomax (SBH)]. Furthermore, other quality parameters were achieved (motility, morphological abnormalities, cytoplasmic droplets, viability, capacitation acrosomal mitochondrial status) their correlations with analysed. Bulls divided into three groups: high [non‐return rate (NRR) ≥ 80],...