Male reproductive success is determined by the ability of males to gain sexual access females and their fertilize ova. Among polygynous mammals, differ markedly in success, a great deal effort has been made understand how selective forces have shaped traits that enhance male competitiveness both before after copulation (i.e., sperm competition). However, possibility also may fertility ignored under assumption infertility rare natural populations because selection against it likely be strong....

Antioxidants could improve sperm media, extending the viability of spermatozoa and protecting their DNA. The protective ability lipoic acid, melatonin, Trolox crocin was tested on red deer incubated at 37°C. Cryopreserved were thawed with 1 mM or 0.1 each antioxidant, without oxidative stress (100 μM Fe2+). Motility (CASA), viability, mitochondrial membrane potential acrosomal status assessed. Lipoperoxidation (malondialdehyde production), intracellular reactive oxygen species (ROS) DNA...

In the present study, computer-automated sperm head morphometry of epididymal samples was used to determine if area and shape are useful measurements for separating "good" "bad" Iberian red deer freezers. A microscope slide prepared from single diluted fresh collected 38 mature stags. Slides were air-dried stained with Hemacolor. The (length/width) a minimum 145 heads determined each male by means Sperm-Class Analyser. remainder sample frozen. After thawing, cryosurvival judged in vitro...

ABSTRACT: The main goal of this study was to investigate the potential protective effects enzymatic and nonenzymatic antioxidants on cryopreservation injuries red deer epididymal spermatozoa. In Experiment 1, sperm freezability catalase, superoxide dismutase, a combination thereof were studied. 2, cryoresistance evaluated when different antioxidants, such as vitamin E, C, butylated hydroxytoluene (BHT), added freezing extender. Sperm quality judged in vitro by microscopic assessments...

Testosterone has been proposed to have a dual effect, enhancing sexual traits while depressing parasite resistance in males. Here, we test this hypothesis red deer, examining males from captive populations during the whole annual cycle and natural breeding season. We first explored effects of body size, age sampling date on testosterone avoid confounding effects. Our results show that seasonal changes levels were mirrored by testes rut there was strong correlation between both. In...

Fe 2 + /ascorbate, hydrogen peroxide (H O ), and hypoxanthine/xanthine oxidase (XOD) are commonly used for inducing oxidative stress on spermatozoa. A comparative study of these agents was carried out thawed spermatozoa from red deer. First, we tested a high, medium, low concentration each agent: 100, 10, 1 μM (hydroxyl radical generator); mM, 10 H ; mU/ml XOD (superoxide generator), incubated at 37 °C 180 min. Intracellular reactive oxygen species (ROS; DCFDA) increased with dose time...

In the present study, we have related mitochondrial membrane potential (DeltaPsim) and forward scatter (FSC) to apoptotic-related changes in spermatozoa. Thawed red deer spermatozoa were incubated synthetic oviductal fluid medium (37 degrees C, 5% CO2), with or without antioxidant (100 microm Trolox). At 0, 3, 6 9 h, aliquots assessed for motility stained a combination of Hoechst 33342, propidium ioide (PI), YO-PRO-1 Mitotracker Deep Red flow cytometry. The proportion YO-PRO-1+ PI+...

Sperm design and velocity play key roles in influencing sperm performance and, therefore, can determine fertilization success. Several interspecific studies have demonstrated how these features correlate, it has been hypothesized that selection may drive changes traits. Here, we examine the association between swimming a study conducted at an intraspecific level Iberian red deer (Cervus elaphus hispanicus). We addressed structure of different subpopulations, based on morphometry velocity,...

The aim of this study was to explore how some reproductive methodologies may affect the sex ratio. We first confirmed association between maturation stage bovine oocytes at time in vitro fertilisation (IVF) and ratio vitro-derived embryos. Secondly, we studied whether insemination, prior or after ovulation, could alter sheep. In experiment, were matured for 16 h; then either fertilised immediately extrusion polar body IVF delayed 8 h. proportion cleaving embryos their development 8-cell...

Long-term storage of semen by cryopreservation, with high recovery rates on thawing, is essential for the establishment genetic resource banks endangered species. The purpose present study was to evaluate various diluents cryopreservation spermatozoa from three species gazelles (genus Gazella) in a captive breeding program. compared were Tes (N-tris(hydroxymethyl)methyl-2 aminoethane sulfonic acid)-Tris 5% egg yolk and 6% glycerol (TEST) Triladyl, yolk-citrate, Tris-trehalose,...

Sperm cryopreservation has become an indispensable tool in biology. Initially, studies were aimed towards the development of efficient freezing protocols different species that would allow for storage semen samples long periods time, ensuring its viability. Nowadays, it is widely known important individual component exists cryoresistance semen, and efforts are at identifying those sperm characteristics may us to predict this cryoresistance. This knowledge lead, ultimately, design optimized...

This study describes milk production and composition of Iberian red deer (Cervus elaphus hispanicus) females (hinds) the effect calving date BW change hinds by milking over 34 wk. All produced throughout 34-wk period, well standard lactation period. Total yield was 224.1 +/- 21.1 L, daily 0.91 0.06 L. Milk decreased with stage (P = 0.01) later a calf born 0.008), it greater in posterior quarters < 0.05). unaffected > 0.10) side position or order udder. did not correlate hind 0.1). Hinds lost...

This study examined the effect of male age, time lapse between death individual and collection its sperm, breeding season (1993, 1995, or 1996), testicle sampled (left right) on cell quality spermatozoa obtained postmortem from epididymis red deer stags (Cervus elaphus hispanicus). A total 142 sperm samples 71 free-ranging individuals shot during were used to investigate these effects. The cauda stags. Immediately after collection, an assessment was made proportion motile (PM), normal...