A5053550432- Reproductive System and Pregnancy
- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- Genetic and phenotypic traits in livestock
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Animal Genetics and Reproduction
- Estrogen and related hormone effects
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- Renal and related cancers
- Pregnancy and preeclampsia studies
- Circular RNAs in diseases
- Tissue Engineering and Regenerative Medicine
- Veterinary Equine Medical Research
- Sperm and Testicular Function
- Milk Quality and Mastitis in Dairy Cows
- Prenatal Screening and Diagnostics
- Genomics and Phylogenetic Studies
- Genetic Syndromes and Imprinting
- Genetic Mapping and Diversity in Plants and Animals
- RNA modifications and cancer
- Birth, Development, and Health
- Kruppel-like factors research
- Epigenetics and DNA Methylation
University of Zurich
2017-2025
Institute of Developmental Physiology
2023
Division of Undergraduate Education
2023
Ludwig-Maximilians-Universität München
2008-2020
ETH Zurich
2014-2020
The Geneva Association
2017
LMU Klinikum
2015
University of Copenhagen
2010
Bavarian State Research Center for Agriculture
2003-2008
Institute of Molecular Biology and Biotechnology
2006-2008
A major challenge in biology is determining how evolutionarily novel characters originate; however, mechanistic explanations for the origin of new are almost completely unknown. The evolution pregnancy an excellent system which to study novelties because mammals preserve stages transition from egg laying live birth. To determine molecular bases this transition, we characterized pregnant/gravid uterine transcriptome tetrapods trace evolutionary history gene expression. We show that thousands...
The endometrium plays a central role among the reproductive tissues in context of early embryo–maternal communication and pregnancy. This study investigated transcriptome profiles samples from day 18 pregnant vs non-pregnant heifers to get insight into molecular mechanisms involved conditioning for embryo attachment implantation. Using combination subtracted cDNA libraries array hybridisation, 109 mRNAs with at least twofold higher abundance animals 70 levels control group were identified....
Although somatic cell nuclear transfer (SCNT) cloning is more efficient in cattle than any other species tested so far, there a high rate of pregnancy failure that has been linked to structural and functional abnormalities the placenta. We hypothesis these changes may originate from disturbed embryo-maternal interactions peri-implantation period. Therefore, we evaluated response endometrium SCNT embryos (produced 7 different fetal fibroblast lines) as compared with derived vitro...
Initiation and maintenance of pregnancy are critically dependent on an intact embryo-maternal communication in the preimplantation period. To get new insights into molecular mechanisms underlying this complex dialog, a holistic transcriptome study endometrium samples from Day 18 pregnant vs. nonpregnant twin cows was performed. This genetically defined model system facilitated identification specific conceptus-induced changes transcriptome. Using combination subtracted cDNA libraries array...
The success of early reproductive events depends on an appropriate communication between gametes/embryos and the oviduct. Extracellular vesicles (EVs) contained in oviductal secretions have been suggested as new players mediating this crucial cross-talk by transferring their cargo (proteins, mRNA small ncRNA) from cell to cell. However, little is known about EVs (oEVS) composition implications success. aim study was determine oEVs content at protein, RNA level examine whether under hormonal...
Stefan Wiemann1,12, Bernd Weil1,2, Ruth Wellenreuther1, Johannes Gassenhuber1,2, Sabine Glassl3, Wilhelm Ansorge3, Michael Böcher4, Helmut Blöcker4, Bauersachs5, Blum5, Jürgen Lauber6, Andreas Düsterhöft6, Beyer7, Karl Köhrer7, Normann Strack2, Hans-Werner Mewes2, Birgit Ottenwälder8, Brigitte Obermaier8, Jens Tampe9, Dagmar Heubner10, Rolf Wambutt10, Bernhard Korn1,11, Michaela Klein1, and Annemarie Poustka1 Molecular Genome Analysis, German Cancer Research Center, 69120 Heidelberg,...
The endometrium plays a central role among the reproductive tissues in context of early embryo–maternal communication and pregnancy. It undergoes typical changes during sexual/oestrous cycle, which are regulated by ovarian hormones progesterone oestrogen. To identify underlying molecular mechanisms we have performed first holistic screen transcriptome bovine intercaruncular at two stages cycle – end day 0 (late oestrus, low progesterone) 12 (dioestrus, high progesterone). A combination...
The oviduct epithelium undergoes marked morphological and functional changes during the oestrous cycle. To study these at level of transcriptome we did a systematic gene expression analysis bovine epithelial cells oestrus dioestrus using combination subtracted cDNA libraries array hybridisation. A total 3072 clones two were analysed by hybridisation with probes derived from six cyclic heifers, three them slaughtered dioestrus. Sequencing cDNAs showing significant differences in their levels...
During the oestrous cycle, bovine endometrium exhibits characteristic morphological and functional changes, which are mainly induced by progesterone (P(4)), oestrogens oxytocin. We studied response of to this changing hormonal environment at transcriptome level using a custom-made cDNA microarray. Endometrium samples were recovered from Simmental heifers on days 0 (oestrus), 3.5 (metoestrus), 12 (dioestrus) 18. The latter group was divided into animals with high (late dioestrus) low P(4)...
Interferon tau (IFNT), a type I IFN similar to alpha IFNs (IFNA), is the pregnancy recognition signal produced by ruminant conceptus. To elucidate specific effects of bovine IFNT and other conceptus-derived factors, endometrial gene expression changes during early were compared after intrauterine application human IFNA2. In experiment 1, tissue samples obtained on Day (D) 12, D15, D18 postmating from nonpregnant or pregnant heifers. 2, heifers treated D14 D16 estrous cycle with an device...
In an attempt to unveil molecular processes controlling the porcine placentation, we have investigated pregnancy-induced gene expression in endometrium using Affymetrix GeneChip Porcine Genome Array. At Day 14 after insemination, at time of initial samples were obtained from pregnant sows and inseminated with inactivated semen. Analysis microarray data revealed 263 genes be significantly differentially expressed between nonpregnant sows. Most ontology terms enriched pregnancy had allocated...
Establishment and maintenance of pregnancy in equids is only partially understood. To provide new insights into early events this process, we performed a systematic analysis transcriptome changes the endometrium at Days 8 12 pregnancy. Endometrial biopsy samples from pregnant nonpregnant stages were taken same mares. Composition collected was analyzed using quantitative stereological techniques to determine proportions surface glandular epithelium blood vessels. Microarray did not reveal...
Summary The objective of this work was to integrate findings from functional genomics studies with genome‐wide association for fertility and production traits in dairy cattle. Association analyses SNPs located within or close 170 candidate genes derived two gene expression the literature were performed. Data 2294 Holstein bulls genotyped 39557 used. A total 111 on chromosomal segments covered by a gene. Allele substitution effects each SNP estimated using mixed model fixed effect marker...
In pigs, conceptus attachment to the uterine surface epithelium starts around Day 14 of pregnancy preceded by a pronounced vascularization at implantation zones, initiating epitheliochorial placentation. To characterize complex transcriptome changes in endometrium course initial attachment, deep sequencing endometrial RNA samples pregnant animals (n = 4) and corresponding cyclic controls was performed using Illumina RNA-Seq. The obtained sequence reads were mapped porcine genome, relative...
The porcine conceptus undergoes rapid differentiation and expansion of its trophoblastic membranes between Days 11 12 gestation. Concomitant with trophoblast elongation, production estrogen, the embryonic pregnancy recognition signal, increases. Conceptus attachment to uterine surface epithelium starts after Day 13, initiating epitheliochorial placentation. To analyze transcriptome changes in endometrium course maternal pregnancy, deep sequencing endometrial RNA samples pregnant animals (n =...
Infertility and subfertility are important pervasive reproductive problems in both domestic animals humans. The majority of embryonic loss occurs during the first three weeks pregnancy cattle women due, part, to inadequate endometrial receptivity for support embryo implantation. To identify heifers contrasting fertility, serial rounds artificial insemination (AI) were conducted 201 synchronized crossbred beef heifers. then fertility classified based on number pregnancies detected day 35 four...
Tightly coordinated, reciprocal embryo-maternal interactions affect gene expression during early pregnancy. Recently, microRNAs (miRNAs) have emerged as new players in the fine tuning of embryo development and implantation mammals via posttranscriptional regulation mechanisms. Here, we integrated transcriptomic computational approaches to profile miRNAs miRNA synthesis transport-related genes at different developmental stages porcine conceptuses trophoblast pregnancy pig. Using...
Subclinical mastitis, the inflammation of mammary gland lacking clinical symptoms, is one most prevalent and costly diseases in dairy farming worldwide. Milk microRNAs (miRNAs) encapsulated extracellular vesicles (EVs) have been proposed as potential biomarkers different conditions, including subclinical mastitis. However, little known about robustness EVs analysis regarding sampling time-point natural infections. To estimate reliability measurements raw bovine milk, we first evaluated...
We studied differential gene expression in ipsilateral and contralateral bovine oviduct epithelial cells using a combination of subtracted cDNA libraries array hybridization. Four Simmental heifers were synchronized slaughtered 3.5 days after they entered standing heat. Epithelial isolated from oviducts. To identify genes that are differentially regulated epithelium, produced by suppression subtractive hybridization analyzed Sequencing cDNAs showing levels epithelium revealed 35 different...
The aim of our study was to analyze endometrial gene expression profiles in ovariectomized cows treated with estradiol and/or progesterone by using microarray analysis. Clustering differentially expressed genes allowed separation into distinct hormone response patterns. These patterns could be classified independent and interdependent actions the steroid hormones estrogen progesterone. use external administration identified a set whose regulation depends on priming effect....
Embryo implantation is a complex, synchronized process that requires establishment of reciprocal dialogue between receptive endometrium and developing blastocysts. Recently, microRNAs (miRNAs), known to modulate gene expression through post-transcriptional mechanisms, were implicated in regulation early pregnancy events including maternal recognition implantation. To characterize complex transcriptomic changes, miRNAs pregnant cyclic endometria collected on days 12, 16 20 was analyzed using...