The aim of this study was to evaluate the capacity domestic cat (Dc, Felis silvestris) oocytes reprogram nucleus cheetah (Ch, Acinonyx jubatus) cells by interspecies SCNT (iSCNT), using embryo aggregation. Dc were in vitro matured and subjected zona pellucida free (ZP-free) or iSCNT, depending on whether donor cell Ch respectively. ZP-free reconstructed embryos then cultured microwells individually (Dc1X Ch1X groups) couples (Dc2X Ch2X groups). Embryo aggregation improved development...

The Iberian lynx (Lynx pardinus) is the most endangered felid in world. Adequate genetic management of situ and ex populations, linkage between both, require knowledge on male reproductive biology factors influencing it. We examined influence age, free-ranging versus captive conditions seasonality phenotypic, endocrine semen traits, links traits fertility. Males had relatively small testes, produced low sperm numbers, a proportion normal sperm, high motile sperm. Young (2-year-old) males...

Contents The aim of this study was to evaluate three different cloning strategies in the domestic cat ( Felis silvestris ) and use most efficient generate wild felid embryos by interspecific iSCNT using Bengal (a hybrid formed cross Prionailurus bengalensis tiger Panthera tigris donor cells. In experiment 1, zona‐free ZP ‐free) resulted higher fusion expanded blastocyst rates with respect zona included techniques that involved or injection cell. 2, ‐free embryo aggregation (2X) were...

Abstract Cell line establishment of somatic cells is a valuable resource to preserve genetic material rare, difficult‐to‐find, endangered and giant species like Jaguar ( Panthera onca ), the largest South American felid. This unit focuses on isolation culture fibroblasts from skin muscle biopsies, ear cartilage dissection immediately after death one several in this biome. These techniques enabled us contribute 570 samples 45 autochthonous species, including Jaguar. The obtained are part...

The aim of this research was to evaluate two different diluents for sperm cryopreservation and study functional parameters in relation the response heparin, lysophosphatidylcholine progesterone, frozen-thawed semen fallow deer (Dama dama) during reproductive season (brama). In way, can be used as a biological model endangered cervids. Semen obtained by electroejaculation. Heparin, progesterone were capacitation acrosome reaction inducers, respectively. Capacitation evaluated...

The morphological and morphometric characterization of spermatozoa has been used as a taxonomic phylogenetic tool for different species mammals. We evaluated compared the sperm morphometry five neotropical primate species: Alouatta caraya, Ateles belzebuth chamek family Atelidae; Cebus cay (=Sapajus cay) nigritus nigritus) Cebidae. After collection semen samples, following parameters were measured on 100 from each specimen: Head Length, Width, Acrosome Midpiece Width Tail Length. Considering...

The pampas deer is an endangered species, from which reproductive biology little known. We aimed to describe and compare the seasonal patterns of adult yearling stags throughout year, including morphological traits, testosterone concentration, sperm morphology cryoresistance pattern changes. Six (AS) five (YS) were captured with anesthetic darts once in winter, spring, summer autumn study variables, serum semen. Adult males heavier, their neck girth tended be greater concentration was higher...

Heterospecific embryo transfer of an endangered species has been carried out using recipients from related domestic females. Aggregation with a tetraploid the to be transferred could improve development pregnancy term. The main objective present study was analyze aggregation in cat model hybrid embryos. For this purpose, we compared vitro synchronic (Sync) or asynchronic (Async) and (Async4n) IVF Furthermore, aggregated blastocyst quality analyzed by evaluation total cell number, allocation...

Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife

Somatic cell nuclear transfer is an assisted reproductive technique that could help to preserve endangered species. Because it difficult obtain wild felid oocytes, interspecific cloning using domestic cat (DC) oocytes alternative produce cloned embryos in these The aim of this study was evaluate different strategies the DC (Felis silvestris catus) and use most efficient strategy generate by cloning. First, we evaluated 3 strategies: (1) enucleation with zona pellucida (ZP) followed fusion a...

The Puma concolor population has been decreasing during the last 30 years. Semen cryopreservation of this species accomplished successfully and offers possibility preserving endangered species. We previously showed that fertilizing capability wild felid spermatozoa can be evaluated using intracytoplasmic sperm injection (ICSI) with in vitro-matured domestic cat oocytes (Moro et al. 2014 Reprod. Domest. Anim. 49, 693-700). Due to lack homologous oocytes, we induce oocyte fertilization...

Even though knowledge in sperm cryopreservation of endangered felids advanced recent years, very little is known about suitable protocols to cryopreserve from Leopardus geoffroyi (LG). In the present study, obtained by electroejaculation 5 different males were cryopreserved either a Tes-Tris- or lactose-based diluent (Gañan et al. 2009 Theriogenology 72, 341-352) with modifications freezing process using one-step method: straws placed horizontally on metal rack, 4cm above surface liquid...

The cheetah (Ch, Acinonyx jubatus) is a species considered globally endangered and cloning one of the assisted reproductive techniques that can help to preserve it study early embryo development. However, production cloned felid embryos remains inefficient, probably because difficulty control process nuclear reprogramming obtain adequate gene expression. Embryo aggregation has been demonstrated improve efficiency in several normalise cdx2 mouse by lowering its expression (Balbach et al....

Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife

Most of the 36 species wild felids are at a level threat, and interspecific SCNT (iSCNT) comes as strategy to contribute these conservation. The aim this study was evaluate effect embryo aggregation in cheetah (Ch, Acinonyx jubatus), bengal (Ben, hybrid between Felis silvestris Prionailurus bengalensis), domestic cat (DC, silvestris) embryos generated by cloning. DC oocytes were vitro matured zona-free (with fibroblasts) or iSCNT Ch Ben performed. reconstructed activated with 5 μM ionomycin...

Tetraploid complementation has been extensively used to verify the pluripotency of stem cells and also for improving placenta formation when tetraploid embryos are aggregated synchronously or asynchronously with diploid (2n) embryos. Generation can be achieved by electric fusion a 2-cell embryo. However, optimal intensity pulse generate not studied in feline. The aims this study were (1) evaluate conditions achieve highest rate without affecting embryo developmental competence, (2) compare...

Fusion of 2-cell embryos generates tetraploid (4n) blastomeres with an increased commitment to trophectoderm. Complementation from endangered species 4n derived a phylogenetically related domestic could improve healthy pregnancy establishment after embryo transfer in females. However, generation, development, and quality complemented felids remain unstudied. Therefore, our objectives were (1) evaluate tetraploidy fused embryos; (2) analyse the blastocyst cell number, distribution synchronic...

The development of assisted reproductive technologies (ARTs) in Felidae species has been proposed to guarantee the genetic viability that are on verge extinction. Investigation sperm cryopreservation and IVF have studied several species, but lack viable homologous oocytes makes assessment fertilization capacity after difficult. We propose evaluate capability Puma concolor (Pc), Leopardus geoffroyi (Lg), Panthera onca (Po) cryopreserved induce domestic cat oocyte subsequent pre-implantation...