A5078959063- Growth Hormone and Insulin-like Growth Factors
- Reproductive Biology and Fertility
- Hypothalamic control of reproductive hormones
- Pituitary Gland Disorders and Treatments
- Animal Genetics and Reproduction
- Birth, Development, and Health
- TGF-β signaling in diseases
- Reproductive System and Pregnancy
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ovarian function and disorders
- Ovarian cancer diagnosis and treatment
- Sperm and Testicular Function
- Sexual Differentiation and Disorders
- Glycosylation and Glycoproteins Research
- Hormonal and reproductive studies
- Neuroendocrine regulation and behavior
- Polyamine Metabolism and Applications
- Estrogen and related hormone effects
- Amino Acid Enzymes and Metabolism
- CRISPR and Genetic Engineering
- Cancer Cells and Metastasis
- Assisted Reproductive Technology and Twin Pregnancy
- Cancer-related gene regulation
- Diversity and Career in Medicine
- Sexuality, Behavior, and Technology
Experimental Medicine and Biology Institute
2015-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2025
Universidad Maimónides
2024-2025
University of Turku
2002-2024
Second Affiliated Hospital of Fujian Medical University
2024
Fujian Medical University
2024
Universidad de Los Andes, Chile
2024
University of Chicago
2024
To assess the consequences of prolonged exposure to elevated levels LH/human chorionic gonadotropin (hCG) in female, we developed a transgenic (TG) mouse model (hCGβ+) that overexpresses hCGβ-subunit cDNA. Because promoter used, ubiquitin C, transgene is expressed multiple tissues, including pituitary gland, which coupling with endogenous common α-subunit results synthesis high bioactive hCG. The TG females presented precocious puberty, infertility, enhanced ovarian steroidogenesis, and...
We previously developed a transgenic (TG) mouse model that overexpresses the human chorionic gonadotropin (hCG) beta-subunit under universal ubiquitin C promoter, displaying in males modest 3-fold increase circulating levels of LH/hCG bioactivity. The were fertile and presented with mild reproductive phenotype. To achieve higher hCG, double TG was generated by cross-breeding hCG beta-expressing mice another line harboring C/common alpha-subunit fusion gene. double-TG expressed excessive...
<b><i>Background/Aims:</i></b> Adult mice lacking functional GABA<sub>B</sub> receptors (GABA<sub>B1</sub>KO) show altered <i>Gnrh1</i> and <i>Gad1</i> expressions in the preoptic area-anterior hypothalamus (POA-AH) females display disruption of cyclicity fertility. Here we addressed whether sexual differentiation brain proper wiring GnRH kisspeptin systems were already disturbed postnatal day 4 (PND4)...
Considerable attention has been paid to the role of sex steroids during periods major skeletal turnover, but interaction gonadotropic hormones, which include LH, FSH, and human chorionic gonadotropin (hCG), within bone tissue have overlooked. The question is pertinent due recent detection extragonadal expression receptors. Western blotting, immunolocalization, RT-PCR supported presence osteoblast LH However, cells failed bind [(125)I]hCG treatment with hCG generate either cAMP or...
Transgenic (TG) mice expressing human chorionic gonadotropin (hCG) beta-subunit under the ubiquitin C promoter, presenting with a moderately elevated level of LH/hCG bioactivity develop multiple neoplasms secondary to endocrine abnormalities, including mammary gland tumors after age 9 months. The increased levels circulating estradiol, progesterone, and prolactin TG females puberty boost lobuloalveolar development in resulting ultimately formation estrogen progesterone receptor-negative,...
The etiology of pituitary adenomas remains largely unknown, with the exception involvement estrogens in formation prolactinomas. We have examined molecular pathogenesis prolactin-producing transgenic female mice expressing human choriongonadotropin (hCG) beta-subunit. LH/CG bioactivity is elevated mice, consequent highly stimulated ovarian progesterone (P(4)) production, face normal estrogen secretion. Curiously, despite levels, large prolactinomas developed these and we provide here several...
Female infertility is often associated with deregulation of hormonal networks, and hyperprolactinemia one the most common endocrine disorders hypothalamic-pituitary axis affecting reproductive functions. We have shown previously that transgenic female mice overexpressing human chorionic gonadotropin β-subunit (hCGβ+ mice), producing elevated levels bioactive LH/hCG, exhibit increased production testosterone progesterone, are overweight infertile, develop pituitary lactotrope adenomas in...
Female transgenic mice that overexpress the human chorionic gonadotrophin β subunit (hCGβ+) develop prolactinomas, whereas hCGβ+ males do not. The high levels of circulating hCG induce massive luteinization in ovary females, and progesterone becomes primary steroid hormone produced, but estradiol remains at physiological level. involvement lactotroph proliferation is not clearly understood; hence, pathogenesis prolactinomas females unclear. TGFβ1 an inhibitor function, reduced activity found...
The metabolic syndrome is a growing epidemic; it increases the risk for diabetes, cardiovascular disease, fatty liver, and several cancers. Several reports have indicated link between hormonal imbalances insulin resistance or obesity. Transgenic (TG) female mice overexpressing human chorionic gonadotropin β-subunit (hCGβ+ mice) exhibit constitutively elevated levels of hCG, increased production testosterone, progesterone prolactin, objective this study was to investigate influence hCG...
Membrane progesterone receptors are known to mediate rapid nongenomic effects in different cell types. Recent evidence revealed that mPRα is highly expressed the rat pituitary, being primarily localized lactotrophs, acting as an intermediary of P4-inhibitory actions on prolactin secretion. The role mPRs prolactinoma development remains unclear. We hypothesize mPR agonists represent a novel tool for hyperprolactinemia treatment. To this end, pituitary expression was studied three animal...