A5080364601- Virus-based gene therapy research
- Cancer Research and Treatments
- Bone and Dental Protein Studies
- Animal Genetics and Reproduction
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Biomedical and Engineering Education
- Amino Acid Enzymes and Metabolism
- Liver physiology and pathology
- CAR-T cell therapy research
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Nuclear Structure and Function
- Nanoparticle-Based Drug Delivery
- Chromosomal and Genetic Variations
- Reproductive Biology and Fertility
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Cholinesterase and Neurodegenerative Diseases
- Sperm and Testicular Function
- Quantum Dots Synthesis And Properties
National University of General San Martín
2016-2023
Consejo Nacional de Investigaciones Científicas y Técnicas
2006-2023
Fundación Instituto Leloir
2006-2023
University of Buenos Aires
2006-2023
Gene Therapy Laboratory
2023
Institute of Astronomy and Space Physics
2006
Sir Charles Gairdner Hospital
1993
Royal Perth Hospital
1993
Abstract The application of new technologies for gene editing in horses may allow the generation improved sportive individuals. Here, we aimed to knock out myostatin ( MSTN ), a negative regulator muscle mass development, using CRISPR/Cas9 and generate edited embryos first time horses. We nucleofected horse fetal fibroblasts with 1, 2 or 5 µg different gRNA/Cas9 plasmids targeting exon . observed that increasing plasmid concentrations mutation efficiency. average efficiency was 63.6% gRNA1...
ABSTRACT Many secretory cells increase the synthesis and secretion of cargo proteins in response to specific stimuli. How couple increased load with a coordinate rise capacity ensure efficient transport is not well understood. We used thyroid stimulated thyrotropin (TSH) demonstrate production thyroid-specific ER–Golgi factors, parallel expansion Golgi complex. TSH also expression CREB3L1 transcription factor, which alone caused amplified factor levels enlargement. Furthermore, potentiated...
The clinical efficacy of conditionally replicative oncolytic adenoviruses (CRAd) is still limited by the inefficient infection tumor mass. Since growth essentially result a continuous cross-talk between malignant and tumor-associated stromal cells, targeting both cell compartments may profoundly influence viral efficacy. Therefore, we developed SPARC promoter-based CRAds since gene expressed in cells cells. These CRAds, expressing or not Herpes Simplex thymidine kinase (Ad-F512...
The homing properties of mesenchymal stromal c`ells (MSCs) toward tumors turn them into attractive tools for combining cell and gene therapy. aim this study was to select in a feasible way human bone marrow-derived MSC subpopulation that might exhibit selective ability target the tumor mass. Using differential vitro adhesive capacities during cells isolation, we selected specific (termed MO-MSCs) exhibited enhanced multipotent capacity increased surface expression integrins (integrins α2,...
Abstract Purpose: A33 antigen is a membrane-bound protein expressed in intestinal epithelium that overexpressed 95% of primary and metastatic colorectal carcinomas but absent most epithelial tissues tumor types. We hypothesized promoter might be useful the design conditionally replicative adenovirus for treatment cancer (CRC). Experimental Design: cloned an fragment (A33Pr) extends from −105 to +307 bp. Using luciferase activity as reporter gene, we showed A33Pr was active CRC cell lines....
Targeting the tumor stroma in addition to malignant cell compartment is of paramount importance achieve complete regression. In this work, we modified a previously designed stroma-targeted conditionally replicative adenovirus (CRAd) based on SPARC promoter by introducing mutated E1A unable bind pRB and pseudotyped with chimeric Ad5/3 fiber (Ad F512v1), assessed its replication/lytic capacity ovary cancer vitro vivo. AdF512v1 was able replicate fresh samples obtained from patients: (i)...
Abstract The successful use of transcriptional targeting for cancer therapy depends on the activity a given promoter inside malignant cell. Because solid human tumors evolve as “cross-talk” between different cell types within tumor, we hypothesized that entire tumor mass might have better therapeutic effect. Secreted protein acidic and rich in cysteine (SPARC) is matricellular overexpressed cancers melanomas both cells compartment stromal one (fibroblasts endothelial cells). We shown...
Decoration of nanoparticles with specific molecules such as antibodies, peptides, and proteins that preserve their biological properties is essential for the recognition internalization target cells. Inefficient preparation decorated leads to nonspecific interactions diverting them from desired target. We report a simple two-step procedure biohybrid containing core hydrophobic quantum dots coated multilayer human serum albumin. These were prepared by ultra-sonication, crosslinked using...
As ancestral haplotypes of the major histocompatibility complex (MHC) appear to define identical MHC in unrelated individuals, individuals sharing same haplotype should also share NK-defined allospecificities that have recently been shown map human MHC. To test this prediction, multiple cell lines from were tested for allospecificities. It was found cells do specificities. Furthermore, phenotype possess two different can be predicted phenotypes are homozygous concerned. Although group 1 and...
Historically, livestock improvement by selective breeding was the principal selection force in animal production and welfare, but desired phenotype may involve more than 1 generation. Nowadays, new technologies such as CRISPR/Cas9 could overpass these limits improve quality insertion or modification of genotype. In this work, we aim to knock out myostatin (MSTN) gene, a negative regulator muscle mass development, equine cells generate cloned embryos with modified To achieve this, 1×105...
Abstract COVID-19 vaccines were originally designed based on the ancestral Spike protein, but immune escape of emergent Variants Concern (VOC) jeopardized their efficacy, warranting variant-proof vaccines. Here, we used preclinical rodent models to establish cross-protective and cross-neutralizing capacity adenoviral-vectored expressing VOC-matched Spike. CoroVaxG.3-D.FR, matched Delta Plus Spike, displayed highest levels nAb VOC mismatched variants. Cross-protection against viral infection...
Supplementary Data from A Novel A33 Promoter–Based Conditionally Replicative Adenovirus Suppresses Tumor Growth and Eradicates Hepatic Metastases in Human Colon Cancer Models
<div>Abstract<p><b>Purpose:</b> A33 antigen is a membrane-bound protein expressed in intestinal epithelium that overexpressed 95% of primary and metastatic colorectal carcinomas but absent most epithelial tissues tumor types. We hypothesized promoter might be useful the design conditionally replicative adenovirus for treatment cancer (CRC).</p><p><b>Experimental Design:</b> cloned an fragment (A33Pr) extends from −105 to +307 bp. Using...
<div>Abstract<p><b>Purpose:</b> A33 antigen is a membrane-bound protein expressed in intestinal epithelium that overexpressed 95% of primary and metastatic colorectal carcinomas but absent most epithelial tissues tumor types. We hypothesized promoter might be useful the design conditionally replicative adenovirus for treatment cancer (CRC).</p><p><b>Experimental Design:</b> cloned an fragment (A33Pr) extends from −105 to +307 bp. Using...
Supplementary Data from A Novel A33 Promoter–Based Conditionally Replicative Adenovirus Suppresses Tumor Growth and Eradicates Hepatic Metastases in Human Colon Cancer Models
<div>Abstract<p>The successful use of transcriptional targeting for cancer therapy depends on the activity a given promoter inside malignant cell. Because solid human tumors evolve as “cross-talk” between different cell types within tumor, we hypothesized that entire tumor mass might have better therapeutic effect. Secreted protein acidic and rich in cysteine (SPARC) is matricellular overexpressed cancers melanomas both cells compartment stromal one (fibroblasts endothelial...
Supplementary Data from Expression of a suicidal gene under control the human secreted protein acidic and rich in cysteine (SPARC) promoter tumor or stromal cells led to inhibition cell growth
<div>Abstract<p>The successful use of transcriptional targeting for cancer therapy depends on the activity a given promoter inside malignant cell. Because solid human tumors evolve as “cross-talk” between different cell types within tumor, we hypothesized that entire tumor mass might have better therapeutic effect. Secreted protein acidic and rich in cysteine (SPARC) is matricellular overexpressed cancers melanomas both cells compartment stromal one (fibroblasts endothelial...
Supplementary Data from Expression of a suicidal gene under control the human secreted protein acidic and rich in cysteine (SPARC) promoter tumor or stromal cells led to inhibition cell growth
SPARC is a matricellular protein that overexpressed in malignant and stromal components of human melanomas. We have previously shown 1.3 Kb promoter region was effective driving suicide gene expression both melanoma endothelial cells leading to the elimination tumors vivo nude mice suggesting could be good candidate for generating conditional replicating oncolytic adenovirus. By using luciferase as reporter gene, we performed detailed analysis activity specificity different fragments...
A33 antigen is a membrane-bound protein that expressed only in intestinal ephitelium and over-expressed most colon cancers. found 95% of primary metastatic cancer cells 38% diffuse gastric cancers with uniform expression but absent other normal tissues tumor types. Based on these characteristics, we hypothesized promoter might be useful the design conditional replicative adenovirus. For this purpose cloned an fragment (A33Pr) includes 5´UTR extends from −105 bp to +307 upstream luciferase...