A5102869681- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Polymer Surface Interaction Studies
- Viral Infections and Immunology Research
- Electrospun Nanofibers in Biomedical Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- CAR-T cell therapy research
- Rheumatoid Arthritis Research and Therapies
- Advanced Drug Delivery Systems
- CRISPR and Genetic Engineering
- Contact Dermatitis and Allergies
- Cancer Research and Treatments
- RNA and protein synthesis mechanisms
- Acne and Rosacea Treatments and Effects
- Nanoplatforms for cancer theranostics
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- Galectins and Cancer Biology
- Clinical Reasoning and Diagnostic Skills
- Animal Genetics and Reproduction
- Nanofabrication and Lithography Techniques
- Innovations in Medical Education
- Mesenchymal stem cell research
William Harvey Research Institute
2014-2025
Queen Mary University of London
2014-2025
Transnational Press London
2020
Weatherford College
2013
City, University of London
2011
Cardiff University
2009
AstraZeneca (United Kingdom)
2009
University of London
2004-2007
Royal Cornwall Hospital
1984-2005
University of Plymouth
2005
Abstract Constitutive expression of the inflammatory cytokine tumor necrosis factor-α (TNF-α) is characteristic malignant ovarian surface epithelium. We investigated hypothesis that this autocrine action TNF-α generates and sustains a network other mediators promote peritoneal cancer growth spread. When compared with two cell lines did not make TNF-α, constitutive production was associated greater release chemokines CCL2 CXCL12, cytokines interleukin-6 (IL-6) macrophage migration-inhibitory...
A survey of 1066 healthy women and 1089 men aged 18-70 years, performed to determine the prevalence facial acne, showed that clinical acne was not confined adolescents. Though it more prevalent among than at 18, beyond age 23 as in gradually declined. At 40-49 years 3% 5% still had definite, albeit mild, 50-59 6% 8% physiological acne. The surprisingly high adults may be related antibiotic treatment or, women, use oral contraceptives or cosmetics, though this did study their influence....
Cultivated murine bone marrow mesenchymal stem cells (MSCs) frequently accumulate chromosome abnormalities, become oncogenically transformed, and generate sarcomas when transplanted in mice. Although human MSCs appear to be more resistant, oncogenic transformation has also been observed cultivated past the senescence phase. Cell therapy for tissue regeneration using autologous requires transplantation of previously expanded vitro. Thus, an important concern is determine if a necessary...
KEYWORDS: Multi-layer hybrid microcapsulesdrug deliveryshell permeabilitytumor therapymulti-functionalizationmesenchymal stem cells
Abstract High‐grade serous ovarian cancer (HGSC) disseminates early and extensively throughout the peritoneal space, causing multiple lesions that are a major clinical problem. The aim of this study was to investigate cellular composition tumour deposits in patient biopsies their evolution mouse models using immunohistochemistry, intravital microscopy, confocal 3D modelling. Tumour from omentum HGSC patients contained prominent leukocyte infiltrate CD3 + T cells CD68 macrophages, with...
Inclusion of iron nanoparticles in the layers microcapsules increases their sedimentation rate and promotes cell delivery bioactive molecules. Importantly, can also be efficiently targeted with a magnet.
Genomic changes affecting tumour suppressor genes are fundamental to cancer. We applied SNP array analysis a panel of testicular germ cell tumours search for novel and identified frequent small deletion on 6q25.3 just one gene, ZDHHC14. The expression ZDHHC14, putative protein palmitoyltransferase with unknown cellular function, was decreased at both RNA levels in tumours. ZDHHC14 also significantly prostate cancer samples lines. In addition our findings genetic clinical samples, inducible...
The design of novel, effective drug delivery systems is one the most promising ways to improve treatment socially important diseases. This article reports on an innovative approach production composite microcontainers (microcapsules) bearing advanced protective functions. Cerium oxide (CeO2) nanoparticles were incorporated into layer-by-layer polyelectrolyte microcapsules as a shell for encapsulated enzyme (luciferase Photinus pyralis), preventing its oxidation by hydrogen peroxide, abundant...
Background Close noticing, as keen discrimination and judgement between qualities, is a key capability for work in visual domains medicine. This generic normally assumed, its specifics are left to develop through experience, traditional apprenticeship specialty. Discrimination an outcome of learning the affective domain, introduces vital aesthetic dimension clinical that aligns with interests medical humanities. An approach reasoning, however, remains largely unexplored explicit focus...
Abstract Background The tumor necrosis factor (TNF)‐α plays a central role in rheumatoid arthritis (RA) and current biotherapies targeting TNF‐α have major impact on RA treatment. long‐term safety concerns associated with the repetitive TNF blockade prompt optimization of therapeutic anti‐TNF approaches. Since we recently demonstrated that intra‐articular gene transfer using recombinant adeno‐associated virus serotype 5 (rAAV5) efficiently transduces arthritic joints, evaluate its effect...
<b>Background:</b> In the context of preclinical development, we studied potential intra-articular gene delivery using a recombinant adeno-associated virus 5 (rAAV5) encoding chimeric human tumour necrosis factorα (TNFα) soluble receptor I linked to mouse immunoglobulin heavy chain Fc portion (TNF I; TNFRI-Ig). <b>Methods:</b> Expression was under control nuclear factor kappa B (NFκB)-responsive promoter and compared with cytomegalovirus (CMV) (rAAV5.NFκB-TNFRI-Ig rAAV5.CMV-TNFRI-Ig,...
The treatment of persistent infections often requires a high local drug concentration and sustained release antimicrobial agents. This paper proposes the use novel electrospinning poly(lactic acid) (PLA) fibers containing uncoated encapsulated chlorhexidine particles. Chlorhexidine particles with mean (SD) diameter 17.15 ± 1.99 μm were fabricated by precipitation diacetate calcium chloride. Layer-by-layer (LbL) encapsulation was carried out to produce had content (90%), when they electrospun...
This work describes the preparation and characterization of printed biodegradable polymer (polylactic acid) capsules made in two different shapes: pyramid rectangular about 1 11 μm size. Obtained core–shell are described terms their morphology, loading efficiency, cargo release profile, cell cytotoxicity, uptake. Both types showed monodisperse size shape distribution were found to provide sufficient stability encapsulate small water-soluble molecules retain them for several days ability...
To demonstrate that posttranslational modification of type II collagen (CII) by reactive oxygen species (ROS), which are known to be present in inflamed arthritic joints, can give rise epitopes specific damaged cartilage rheumatoid arthritis (RA) and osteoarthritis (OA) establish a proof concept antibodies ROS-modified CII used target therapeutics specifically joints.We semisynthetic phage display human antibody library raise single-chain variable fragments (scFv) CII. The specificity...
Achieving localized delivery of small molecule drugs has the potential to increase efficacy and reduce off target side effects associated with systemic distribution. Herein, we explore use layer-by-layer (LbL) assembled microcapsules for doxycycline. Absorbance doxycycline onto core dextran sulfate preassembled provides an efficient method load both synthetic biodegradable drug. Application outer layer lipid coat enhances sustained in vitro release from microcapsule types. To monitor a...
The clinical efficacy of conditionally replicative oncolytic adenoviruses (CRAd) is still limited by the inefficient infection tumor mass. Since growth essentially result a continuous cross-talk between malignant and tumor-associated stromal cells, targeting both cell compartments may profoundly influence viral efficacy. Therefore, we developed SPARC promoter-based CRAds since gene expressed in cells cells. These CRAds, expressing or not Herpes Simplex thymidine kinase (Ad-F512...
Microcapsules of customized shapes offer significant advantages over spherical ones, including enhanced internalization by host cells, improved flow characteristics, and higher packing capacity. In our work, we propose a method for defined-shape polymer capsules fabrication inspired traditional "pelmeni" (dumplings) making process. The proposed is based on soft lithography technique. Two different approaches were demonstrated resulting in polyelectrolyte multilayer poly(lactic acid) (PLA)...