A5089202572- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Bone fractures and treatments
- Bone Tissue Engineering Materials
- Osteoarthritis Treatment and Mechanisms
- Periodontal Regeneration and Treatments
- RNA Interference and Gene Delivery
- Dendrimers and Hyperbranched Polymers
- Polymer Surface Interaction Studies
- Cell Adhesion Molecules Research
- TGF-β signaling in diseases
- Spine and Intervertebral Disc Pathology
- Electrospun Nanofibers in Biomedical Applications
- Corneal Surgery and Treatments
- Wastewater Treatment and Nitrogen Removal
- Connective tissue disorders research
- Fibroblast Growth Factor Research
- Water Quality Monitoring and Analysis
- Knee injuries and reconstruction techniques
- Cellular Mechanics and Interactions
- Proteoglycans and glycosaminoglycans research
- Neurobiology and Insect Physiology Research
- Advanced oxidation water treatment
- Advanced Polymer Synthesis and Characterization
- Developmental Biology and Gene Regulation
Universidad de Málaga
2010-2022
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2011-2022
Instituto de Investigación Biomédica de Málaga
2015-2022
Universidad de Cádiz
2021-2022
Andalusian Centre for Nanomedicine and Biotechnology
2021-2022
Medical Research Network
2018
Pediatrics and Genetics
2010-2016
Centro de Investigación Biomédica en Red
2016
University of Southern California
1996-1999
Brooklyn College
1997
Keratoconjunctivitis sicca (KCS) or dry eye disease (DED) is an immune-mediated multifactorial disease, with high level of prevalence in humans and dogs. Our aim this study was to investigate the therapeutic effects allogeneic adipose-derived mesenchymal stromal cells (Ad-MSCs) implanted around lacrimal glands 12 dogs (24 eyes) KCS, which refractory current available treatments. Schirmer tear test (STT) ocular surface integrity were assessed at 0 (before treatment), 3, 6, 9 months after...
Abstract MicroRNAs, small noncoding RNAs, regulate gene expression primarily at the posttranscriptional level. We previously found that miR-335 is critically involved in regulation and differentiation capacity of human mesenchymal stem cells (hMSCs) vitro. In this study, we investigated significance for therapeutic potential hMSCs. Analysis hMSCs ex vivo culture demonstrated a significant progressive increase prevented by telomerase. Expression levels were also positively correlated with...
Biogenic production of sulfide in wastewater treatment plants involves odors, toxicity and corrosion problems. The is a consequence bacterial activity, mainly sulfate-reducing bacteria (SRB). To prevent this production, the efficiency nitrate addition to was tested at plant-scale by dosing concentrated calcium (Nutriox) works inlet. Nutriox resulted sharp decrease sulfide, both air bulk water, reaching maximum decreases 98.7% 94.7%, respectively. Quantitative molecular microbiology...
Feline eosinophilic keratitis (FEK) is a chronic keratopathy caused by suspected immune mediated response to an unknown antigenic stimulus. The purpose of this study was investigate the safety and therapeutic effects allogeneic feline adipose-derived mesenchymal stromal cells (fAd-MSCs) implanted subconjunctival around ocular surface lesion in five cats with FEK refractory current available treatments. diagnosed clinical appearance evidence eosinophil and/or mast corneal cytology. Each...
Bone maintenance requires a continuous source of osteoblasts throughout life. Its remodeling and regeneration during fracture repair is ensured by osteoprogenitor stem cells which are part the stroma bone marrow (BM). Many investigators have reported that in cultured BM stromal there cell population will differentiate along an osteogenic lineage if stimulated addition inducers, such as dexamethasone (dex), beta-glycerophosphate (beta-GP), transforming growth factor beta-1 (TGF-beta 1)...
Recent studies on the morphogenesis of fins Danio rerio (zebrafish) during development and regeneration suggest that a number inductive signals involved in process are similar to some those affect bone cartilage differentiation mammals humans. Akimenko et al. (2002) has shown morphogenetic protein-2b (BMP2b) is induction dermal fin regeneration. Many other groups have also molecules from transforming growth factor-beta superfamily (TGFβ), including BMP2, effective promoting chondrogenesis...
In vivo testing is a mandatory last step in scaffold development. Agile longitudinal noninvasive real-time monitoring of stem cell behavior biomaterials implanted live animals should facilitate the development scaffolds for tissue engineering. We report on bioluminescence imaging (BLI) procedure simultaneous changes expression multiple genes to evaluate performance vivo. Adipose tissue-derived stromal mensenchymal cells were dually labeled with Renilla red fluorescent protein and firefly...
Articular cartilage (AC) is an avascular tissue with precise polarity and organization. The three distinct zones are: surface, middle deep. production accumulation of the superficial zone protein (SZP), also known as lubricin, by surface a characteristic feature AC. To date, there wealth evidence showing differentiation AC from mesenchymal stem cells. Most studies that described chondrogenic did not focus on marker SZP/lubricin. present investigation was initiated to determine induction...
AbstractBasic fibroblast growth factor (bFGF) is a potent in vitro mitogen for capillary endothelial cells, stimulates angiogenesis vivo, and may participate tissue repair. Basic FGF found abundance tissues such as brain, kidney cartilage. This study reports the expression, purification, renaturation of biologically active human basic fusion protein (hbFGF-F1) from Escherichia coli. A prokaryotic expression vector was engineered to produce tripartite consisting (i) purification tag, (ii)...
Mesenchymal stem cells give rise to the progenitors of many differentiated phenotypes, including osteocytes, chrondocytes, myocytes, adipocytes, fibroblasts, and marrow stromal cells, which are capable self-renewal undergo expansion in presence transforming growth factor-β1 (TGF-β1). The present study was designed test concept that mesenchymal progenitor could be selected expanded by virtue their intrinsic physiologic responses TGF-β1. Human bone aspirates were initially cultured, under low...
Stem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a useful strategy. Although MSCs are commonly isolated by exploiting their plastic adherence, several studies have suggested that there other populations of and/or osteoprogenitor removed from primary culture during media replacement. Therefore, we developed three-dimensional (3D) system in which adherent and nonadherent selected expanded. Here, described the characterization 3D culture-derived vitro capacity...
Mesenchymal stem cells (MSCs) are promising candidates for a range of tissue regeneration applications.