Keratoconjunctivitis sicca (KCS) or dry eye disease (DED) is an immune-mediated multifactorial disease, with high level of prevalence in humans and dogs. Our aim this study was to investigate the therapeutic effects allogeneic adipose-derived mesenchymal stromal cells (Ad-MSCs) implanted around lacrimal glands 12 dogs (24 eyes) KCS, which refractory current available treatments. Schirmer tear test (STT) ocular surface integrity were assessed at 0 (before treatment), 3, 6, 9 months after...

The two main sources of mesenchymal stem cell (MSCs) in the canine species are bone marrow (cBM-MSCs) and adipose tissue (cAd-MSCs). secretion multitude bioactive molecules, included under concept secretome found cultured medium, play a predominant role mechanism action these cells on regeneration. Although certain features its characterization well documented, their secretory profiles remain unknown. We described compared, for first time, profile exosomes standard monolayer culture MSCs...

Canine atopic dermatitis (AD) is a common skin disease with 10–15 per cent prevalence. Current treatments vary in their efficacy and safety. The immunomodulatory properties of mesenchymal stem cells (MSCs) make them promising alternative treatment. aim this study was to evaluate the therapeutic safety allogeneic canine adipose MSCs (cAd‐MSCs) dogs refractory AD. Twenty‐six dogs, suffering from AD for at least 12 months, not responding conventional therapy, received an intravenous dose 1.5×10...

Feline eosinophilic keratitis (FEK) is a chronic keratopathy caused by suspected immune mediated response to an unknown antigenic stimulus. The purpose of this study was investigate the safety and therapeutic effects allogeneic feline adipose-derived mesenchymal stromal cells (fAd-MSCs) implanted subconjunctival around ocular surface lesion in five cats with FEK refractory current available treatments. diagnosed clinical appearance evidence eosinophil and/or mast corneal cytology. Each...

SUMMARY Thirty healthy male horses were allotted to 3 groups and treated blindly during 4 days. Group-1 received unfractioned calcium heparin (100 IU/kg of body weight, sc , q 12 h). Group-2 a single dose low-molecular-weight (50 anti-X IU/kg, ) every morning, similar volume saline solution evening. Group-3 the vehicle (saline solution), hours. Citrated edta -anticoagulated blood samples collected before starting medication (T-0) once daily hours after each morning injection (T-3, T-27,...

During 1992, a widespread outbreak of Equine viral arteritis (EVA) occurred at riding establishment near Barcelona, Spain. A total 31 out 186 horses on the premises displayed clinical signs, most frequently, fever, depression, mild ventral and limb oedema vesicular-erosive stomatitis, with hypersalivation, petechiations small ulcerations. Affected developed illness varying severity only few exhibiting severe form disease no mortality was recorded. Haematological blood biochemical examination...

Abstract Background Canine colostrum milk (CCM) is a specific secretion of the mammary gland that fundamental for survival newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. Results A characterization exosomes was performed. Exosomes were abundant in and appeared with characteristic cup-shaped morphology well-defined round vesicles. The size distribution between 37 140 nm, western blot analysis showed...

Limbal stem cells (LSCs) are a quiescent cell population responsible for the renewal of corneal epithelium. Their deficiency is conjunctivization cornea that seen in different ocular pathologies, both humans and canine species. The species represents an interesting preclinical animal model surface pathologies. However, role LSCs physiological pathological conditions not well understood. Our objective was to characterize first time soluble factors proteomic profile secretome exosomes (cLSCs)....

<title>Abstract</title> Background : Canine colostrum milk (CCM) is a specific secretion of the mammary gland that fundamental for survival newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. Results A characterization exosomes was performed. Exosomes were abundant in and appeared with characteristic cup-shaped morphology well-defined round vesicles. The size distribution between 37 140 nm, western blot...

The role played by certain domestic species such as dogs a translational model in comparative oncology shows great interest to develop new therapeutic strategies brain tumors. Gliomas are challenge that represents the most common form of malignant primary tumors humans and second dogs. Gene-directed enzyme/prodrug therapy using adipose mesenchymal stem cells (Ad-MSCs) expressing herpes simplex virus thymidine kinase (TK) has proven be promising alternative glioblastoma therapy, through its...

Mesenchymal stem cells (MSCs) have been shown to therapeutic efficacy in different complex pathologies feline species. This effect is attributed the secretion of a wide variety bioactive molecules and extracellular vesicles, such as exosomes, with significant paracrine activity, encompassed under concept secretome. However, at present, exosomes from MSCs not yet studied detail. The objective this study analyze compare protein profiles secretome whole its exosomal fraction adipose-derived...

Feline chronic gingivostomatitis (FCGS) is a pathology with complicated therapeutic approach and prevalence between 0.7 12%. Although the etiology of disease diverse, feline calicivirus infection known to be predisposing factor. To date, available treatment helps in controlling disease, but cannot always provide cure, which leads high percentage refractory animals. Mesenchymal stem cells (MSCs) play pivotal role homeostasis reparation different tissues have ability modulate immune system...

The feline calicivirus (FCV) causes infections in cats all over the world and seems to be related a broad variety of clinical presentations, such as chronic gingivostomatitis (FCGS), severe oral pathology cats. Although its etiopathogeny is largely unknown, FCV infection likely main predisposing factor for developing this pathology. During recent years, new strategies treating FCGS have been proposed, based on use mesenchymal stem cells (MSC) their regenerative immunomodulatory properties....

Staphylococcus pseudintermedius and aureus are common colonizing pathogens in companion animals. These opportunistic can cause infections of varying frequency severity humans pets. Studies on colonization veterinarians scarce. This study aimed to investigate the nostrils hands by S. aureus, epidermidis, among healthy clinical practice province Malaga (Spain), with a particular focus their potential antibiotic resistance. A request for voluntary participation was extended professionals from...

Mesenchymal stem cell (MSC) secretome refers to a variety of bioactive compounds that represents the more important pathway by which MSCs participate in tissue regeneration. Many these have shown variable functional activity even across nonmammalian vertebrate species, although turtles not yet been described. Canine adipose MSC has successfully used experimentally skin healing. Our aim was conduct blinded controlled study evaluate effect canine (cS-MSC) as an alternative for healing soft...

Abstract Background: Colostrum is a specific secretion of the mammary gland fundamental for survival puppy during first weeks after birth. It contains important bioactive molecules involved in passive immunity and maturation various organs, highlighting small vesicles named exosomes. Exosomes have not been described yet canine colostrum milk, where its role crucial dam-newborn communication development neonate. Results: were abundant milk appeared with characteristic cup-shaped morphology...

Abstract Background Colostrum is a specific secretion of the mammary gland fundamental for survival puppy during first weeks after birth. It contains important bioactive molecules involved in passive immunity and maturation various organs, highlighting small vesicles named exosomes. Exosomes have not been described yet canine colostrum milk, where its role crucial dam-newborn communication development neonate. Results were abundant milk appeared with characteristic cup-shaped morphology...

Abstract Background: Canine colostrum milk (CCM) is a specific secretion of the mammary gland that fundamental for survival newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. Results: A characterization exosomes was performed. Exosomes were abundant in and appeared with characteristic cup-shaped morphology well-defined round vesicles. The size distribution between 37 140 nm, western blot analysis showed...

Abstract Background : Canine colostrum milk (CCM) is a specific secretion of the mammary gland that fundamental for survival newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. Results A characterization exosomes was performed. Exosomes were abundant in and appeared with characteristic cup-shaped morphology well-defined round vesicles. The size distribution between 37 140 nm, western blot analysis showed...

Abstract Background : Canine colostrum milk (CCM) is a specific secretion of the mammary gland that fundamental for survival newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. Results A characterization exosomes was performed. Exosomes were abundant in and appeared with characteristic cup-shaped morphology well-defined round vesicles. The size distribution between 37 140 nm, western blot analysis showed...

<title>Abstract</title> Background: Canine colostrum milk (CCM) is a specific secretion of the mammary gland fundamental for survival newborn. It has many described components (immunoglobulins, proteins or fat), but small vesicles named exosomes are largely unknown. Results: A characterization CCM been performed. Exosome concentrations were abundant in and appeared with characteristic cup-shaped morphology well-defined round vesicles. Their size distribution was between 37−140 nm western...