A5066643987- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Aquaculture Nutrition and Growth
- Fecal contamination and water quality
- Identification and Quantification in Food
- Salmonella and Campylobacter epidemiology
- Aquatic life and conservation
- Legionella and Acanthamoeba research
- Invertebrate Immune Response Mechanisms
- Marine Bivalve and Aquaculture Studies
- Plant Pathogenic Bacteria Studies
- Seaweed-derived Bioactive Compounds
- Water Treatment and Disinfection
- Clostridium difficile and Clostridium perfringens research
- Pharmaceutical and Antibiotic Environmental Impacts
- Indoor Air Quality and Microbial Exposure
- Child Nutrition and Water Access
- Forensic Entomology and Diptera Studies
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Echinoderm biology and ecology
- Water Quality Monitoring Technologies
- Bacterial biofilms and quorum sensing
- Reproductive biology and impacts on aquatic species
- Dietary Effects on Health
Universidad de Málaga
2007-2024
Instituto de Investigación Biomédica de Málaga
2023
Bacteria isolated from an outbreak with moderate mortalities in farmed sole, Solea senegalensis (Kaup), the south of Spain were identified as Vibrio harveyi and V. parahaemolyticus. Only bacterial strains showing swarming virulent sole caused experimentally inoculated fish. However, signs disease only reproduced harveyi. The intramuscular inoculation extracellular products (ECPs) both species produced fish appearance surface ulcers case sublethal doses ECPs to showed a protective effect against
One of the main bioactive compounds interest from Ulva species is sulfated polysaccharide ulvan, which has recently attracted attention for its anticancer properties. This study investigated cytotoxic activity ulvan polysaccharides obtained rigida in following scenarios: (i) vitro against healthy and carcinogenic cell lines (1064sk (human fibroblasts), HACAT (immortalized human keratinocytes), U-937 (a leukemia line), G-361 malignant melanoma), HCT-116 colon cancer line)) (ii) vivo zebrafish...
Abstract The protection of cultured sole, Solea senegalensis , against Vibrio harveyi and Photobacterium damselae subsp. piscicida was evaluated following the use a divalent vaccine prepared with formalized whole cells extracellular products virulent strains both pathogenic microorganisms administered by immersion route. Two prolonged immersions 5–10 g fish in bacterin at 1‐month interval gave high levels similar to those obtained when respective monovalent vaccines were intraperitoneal...
Zoonotic parasitic diseases are considered a global threat to public health. In this sense, canines and felines may be infected by different cosmopolitan parasites, with playgrounds serving as an important focus of infection for humans, well domestic or wild animals. Knowledge the epidemiological situation parasites in animal reservoirs integrated into environment, identifying spread pathways, is key element effective response threat. Thus, aim study was assess frequency intestinal zoonotic...
Several characteristics, including biochemical, serological, drug resistance and plasmid profiles, of strains isolated from clams in southwestern Spain affected with brown ring disease have been comparatively studied.On the basis 36 standard physiological biochemical tests, all were included genus Vibno further divided into 6 groups.The groups differentiated on only 8 phenotypic traits: growth at 35 "C, arginine dihydrolase, gelatinase production, Voges-Proskauer ONPG acid production...
Several virulence factors involved in the potential pathogenic capacity of Vibrio P1, causative agent brown ring disease (BRD) affecting cultured Manila clam adults (Ruditapes philippinarum), have been evaluated comparison with other strains several species isolated from diseased clams. The importance bacterial cell surface associated properties as has studied considering both non-specific and specific adhesion to P1 showed moderate hydrophobicity, but high affinity bind Congo Red dye...
To characterize 16 Vibrio harveyi strains isolated from different epizootic outbreaks affecting farmed Senegalese sole.The tested have broad phenotypic diversity based on their biochemical and exoenzymatic patterns, outer membrane proteins (OMP), extracellular product (ECP) patterns presence of prophages. Lethal dose 50 (LD(50)) the in vitro antagonism tests with two probiotic were also determined. The OMP analysis revealed three (A, M V). electrophoretic ECP showed groups. All considered...
The thick-lipped grey mullet (
Two beaches at Malaga, Spain, (one clearly polluted, one a “blue flag” beach) were studied to establish their microbiological status; epidemiological studies carried out identify any association between use of the and enteric or non-enteric diseases. Correlations then sought results two studies. Skin infections found be associated with seawater levels P. aeruginosa , C. albicans A. hydrophila which could related faecal contamination.
Summary The plasmid profiles, and their association with antimicrobial resistance, of 60 strains Aeromonas hydrophila isolated from fish, shellfish water were investigated. Only two susceptible to all the agents tested; highest incidences resistance tetracycline (96.7%), prystanamycin (93.3%), ampicillin (91.7%) cephalothin (91.7%). Forty harboured one or more plasmids profile most frequently detected (15%) was three small 4.2, 3.2 2.8 Mda. Curing experiments indicated that plasmid-free...
Different serotypes of salmonellas were compared for selectivity and efficiency recovery using 11 plating media. No optimal growth was obtained after 24h incubation in any the media, but 48h, brilliant green, green‐phenol red‐lactose‐sucrose, bismuth sulphite, xylose‐lysine‐deoxycholate Hektoen enteric agars showed all salmonella serotypes. Xylose‐lysine‐deoxycholate red‐lactose‐sucrose most selective media Addition 10 μg/ml sodium novobiocin to tryptic soy‐xylose‐lysine soy‐brilliant green...
Abstract The ability of a set Photobacterium damselae ssp. piscicida strains isolated from different fish species to produce superoxide dismutase (SOD) and catalase enzymes was determined. Unlike other bacterial pathogens, P. is not able isoforms SOD or containing metal cofactors when cultured under oxidative stress induced by hydrogen peroxide methyl viologen, iron depleted conditions. However, content the growth medium influenced levels activity in cells, these decreasing with availability...
A comparative study of the phage types, antimicrobial susceptibility patterns, and plasmid profiles 171 strains Salmonella isolated from food, epidemic outbreaks, water-contaminated environments as well sporadic human isolates was carried out to determine most adequate marker in epidemiological investigations. Typing based on appears be effective method for grouping with same serotype obtained a single outbreak environmental sources. However, none three markers tested allow us total...
Given the potential of algae as a new aquafeed ingredient, this study evaluated effect tank-cultivated macroalgae Ulva rigida C. Agardh (Chlorophyta) on innate immune response, intestinal microbiota and their resistance against Photobacterium damselae subsp. piscicida in juvenile gilthead seabream (Sparus aurata L.) after short pulse administration. An algae-free diet was used control, an experimental formulated containing 25% U. (UL-25). After 2, 7, 15 30 days biological samples were taken....
In fish culture settings, the exogenous input of steroids is a matter concern. Recently, we unveiled that in gilthead seabream ( Sparus aurata ), G protein-coupled estrogen receptor agonist G-1 (G1) and endocrine disruptor 17α-ethinylestradiol (EE 2 ) are potent modulators polyreactive antibody production. However, integral role microbiota upon immunity processing response to effect EE remains largely unexplored. Here, juvenile seabreams continuously exposed for 84 days oral G1 or mixed food...
The selectivity and efficiency of several enrichment broths used for the detection salmonellas were comparatively evaluated under laboratory environmental conditions. Media with selenite less efficient in their inhibition growth Gram‐positive micro‐organisms. Salmonellas grew slowly tetrathionate broth media containing brilliant green. These inhibited Salmonella typhi, which only selenite. results obtained experiments stressed indicate that F, F novobiocin, cystine Rappaport‐Vassiliadis...
The present study was carried out to determine the bioactivity of polysaccharides extracted from Euglena gracilis (EgPs). These were characterized by FT-IR and GC-MS. Cytotoxicity analyses (MTT) performed on healthy human gingival fibroblast cell lines (HGF-1), obtaining an IC50 228.66 µg mL−1, with anticancer activity for colon cancer (HCT-116), breast (MCF-7), leukemia (U-937, HL-60) lung (NCl-H460), showing that EgPs have activity, mainly in HTC-116 cells (IC50 = 26.1 mL−1). immunological...