A5034448567- Neurobiology and Insect Physiology Research
- Neurogenesis and neuroplasticity mechanisms
- Memory and Neural Mechanisms
- Neuroscience and Neuropharmacology Research
- Cephalopods and Marine Biology
- Neural dynamics and brain function
- Neuroendocrine regulation and behavior
- Olfactory and Sensory Function Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Axon Guidance and Neuronal Signaling
- Receptor Mechanisms and Signaling
- Pain Mechanisms and Treatments
- Nicotinic Acetylcholine Receptors Study
- Zebrafish Biomedical Research Applications
- Alzheimer's disease research and treatments
- RNA Research and Splicing
- Insect and Pesticide Research
- Photoreceptor and optogenetics research
- Neuropeptides and Animal Physiology
- Gene Regulatory Network Analysis
- MicroRNA in disease regulation
- Stress Responses and Cortisol
- Pharmacological Effects and Assays
- Animal testing and alternatives
- Ion Channels and Receptors
Biomedicine Research Institute of Buenos Aires - CONICET - Partner Institute of the Max Planck Society
2010-2024
Fundación Instituto Leloir
2011-2016
Consejo Nacional de Investigaciones Científicas y Técnicas
2011-2016
University of California, San Diego
2002-2009
Fundación Ciencias Exactas y Naturales
2000-2002
University of Buenos Aires
2000-2002
University of Würzburg
2000
The adult dentate gyrus generates new granule cells (GCs) that develop over several weeks and integrate into the preexisting network. Although hippocampal neurogenesis has been implicated in learning memory, specific role of GCs remains unclear. We examined whether immature adult-born neurons contribute to information encoding. By combining calcium imaging electrophysiology acute slices, we found weak afferent activity recruits few mature while activating a substantial proportion neurons....
The etiology of Parkinson's disease (PD) converges on a common pathogenic pathway mitochondrial defects in which α-Synuclein (αSyn) is thought to play role. However, the mechanisms by αSyn and its disease-associated allelic variants cause dysfunction remain unknown. Here, we analyzed axonal transport morphology human-derived neurons overexpressing wild-type (WT) or mutated A30P A53T, are known have differential lipid affinities. A53T was enriched fractions, inducing significant...
Considerable progress has been made in understanding the mechanisms that control production of specialized neuronal types. However, how timing differentiation contributes to diversity developing spinal cord is still a pending question. In this study, we show CerebroSpinal Fluid-contacting Neurons (CSF-cNs), an anatomically discrete cell type ependymal area, originate from surprisingly late neurogenic events ventral cord. CSF-cNs are identified by expression transcription factors Gata2 and...
Circular RNAs (circRNAs) are a large class of noncoding RNAs. Despite the identification thousands circular transcripts, biological significance most them remains unexplored, partly because lack effective methods for generating loss-of-function animal models. In this study, we focused on circTulp4, an abundant circRNA derived from Tulp4 gene that is enriched in brain and synaptic compartments. By creating circTulp4-deficient mouse model, which mutated splice acceptor site responsible...
Olfaction is influenced by contextual factors, past experiences, and the animal's internal state. Whether this information integrated at initial stages of cortical odour processing not known, nor how these signals may influence encoding. Here we revealed multiple diverse non-olfactory responses in primary olfactory (piriform) cortex (PCx), which dynamically enhance PCx discrimination according to behavioural demands. We performed recordings neurons from mice trained a virtual reality task...
Neuronal activity-dependent gene expression is fundamental to a wide variety of brain functions. However, how development progress and stimulation modalities specifically affect neuron transcription not fully understood. In this work, we first investigate the influence on neuronal firing activity-driven transcription. We used an RNA sequencing approach over 7 days in vitro (DIV) or mature 21 DIV neurons, comparing depolarization with potassium chloride (KCl) versus Biccuculine application,...
Adult hippocampal neurogenesis provides the dentate gyrus with heterogeneous populations of granule cells (GC) originated at different times. The contribution these to information encoding is under current investigation. Here, we show that incoming spike trains activate GC determined by stimulation frequency and age. Immature respond a wider range stimulus frequencies, whereas mature are less responsive high frequencies. This difference dictated feedforward inhibition, which restricts...
Significance Schizophrenia and bipolar disorder are psychiatric syndromes with a significant social economic burden that share common symptom, psychosis, attributed to excessive dopamine release. Despite years of intensive research, the causes these devastating diseases still unknown. In this work, mouse line selective deletion molecular target antipsychotics, D 2 receptors, from most affected neuron subtype in patients, parvalbumin interneurons, results animals schizophrenia-like phenotypes...
Highlights•Spinal cord cerebrospinal fluid-contacting neurons (CSF-cNs) are late-born neurons•CSF-cNs derive from Ascl1-expressing ventral progenitors•Delayed Ascl1 initiates CSF-cNs differentiation and suppresses ependymogenesis•Ascl1 controls neuronal non-neuronal composition of the spinal central canalSummaryGeneration types at right time, location, number is essential for building a functional nervous system. Significant progress has been reached in understanding mechanisms that govern...
Neuronal circuits form during embryonic life, even before synapses are completely mature. Developmental changes can be quantitative (e.g., connections become stronger and more reliable) or qualitative form, lost, switch from electrical to chemical excitatory inhibitory). To explore how these synaptic events contribute behavioral circuits, we have studied the formation of a circuit that produces local bending (LB) behavior in leech embryos. This is composed three layers neurons:...
The dentate gyrus (DG) of the hippocampus plays a key role in memory formation, and it is known to be modulated by septal projections. By performing electrophysiology optogenetics, we evaluated cholinergic modulation processing afferent inputs DG. We show that mature granule cells (GCs), but not adult-born immature neurons, have increased responses perforant path stimuli upon modulation. This due highly precise reconfiguration inhibitory circuits, differentially affecting Parvalbumin...
Abnormal tau accumulation is the hallmark of several neurodegenerative diseases, named tauopathies. Strategies aimed at reducing in brain are promising therapeutic interventions, yet more precise therapies would require targeting specific nuclei and neuronal subpopulations affected by disease while avoiding global reduction physiological tau. Here, we developed artificial microRNAs directed against human MAPT mRNA to dwindle protein engaging endogenous RNA interference pathway. In...
Abstract The heat‐transducing receptor VR1 cloned from rat sensory neurons can be activated by both noxious heat and capsaicin. As the response of to capsaicin is species dependent, it conceivable that responses are transduced distinct receptors across different species. Therefore, we investigated a capsaicin‐insensitive (chick) capsaicin‐sensitive (rat) In chick, whole‐cell patch‐clamp experiments in isolated dorsal root ganglion revealed two populations with thresholds heat, at ≈ 43 °C 53...
Abstract Primary sensory cortices, initially considered elementary encoders of physicochemical attributes environmental stimuli, are now known to be modulated by other aspects experience, such as attentional state and internal expectations 1–3 , movement-related signals 4–7 spatial information 2, 8, 9 . However, the specific role these in cortical processing is not fully understood 10 Here we reveal multiple diverse non-olfactory responses primary olfactory (piriform) cortex (PCx), which...
Abstract Odours are key signals for guiding spatial behaviours such as foraging and navigation in rodents. It has recently been found that odour representations the piriform cortex (PCx) can also contain information about their context. However, precise origins of this within brain its subsequent integration into microcircuitry PCx remains unknown. In study, we focus on lateral entorhinal (LEC) a candidate carrying contextual to PCx, investigate how it affects microcircuit response olfactory...
Odors are key signals for guiding spatial behaviors such as foraging and navigation in rodents. Recent findings reveal that odor representations the piriform cortex (PCx) also encode context information. However, brain origins of this information its integration into PCx microcircuitry remain unclear. This study investigates lateral entorhinal (LEC) a potential source contextual affecting microcircuit olfactory responses. Using mice slices, we performed patch-clamp recordings on superficial...