A5004367419- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Visual perception and processing mechanisms
- Neurobiology and Insect Physiology Research
- Advanced Memory and Neural Computing
- Receptor Mechanisms and Signaling
- Molecular Communication and Nanonetworks
- Photochromic and Fluorescence Chemistry
- Scientific Research and Discoveries
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion channel regulation and function
- Structural Health Monitoring Techniques
- CCD and CMOS Imaging Sensors
- Control Systems and Identification
- Ultrasound and Cavitation Phenomena
- Cell Image Analysis Techniques
- Advanced Optical Imaging Technologies
- Retinal Development and Disorders
- Digital Holography and Microscopy
- Optical Imaging and Spectroscopy Techniques
- Optical Coherence Tomography Applications
- Ultrasound and Hyperthermia Applications
University of California, Berkeley
2016-2025
Berkeley College
2023-2025
Neuroscience Institute
2020-2024
Chan Zuckerberg Initiative (United States)
2023
Institute of Molecular and Cell Biology
2020
University of North Carolina at Chapel Hill
2020
University of California, San Francisco
2004-2019
Purdue University West Lafayette
2019
Neurosciences Institute
2017
University of California, San Diego
2009-2012
Abnormally synchronized synaptic transmission in the brain causes epilepsy. Most inherited forms of epilepsy result from mutations ion channels. However, one form epilepsy, autosomal dominant partial with auditory features (ADPEAF), is characterized by a secreted neuronal protein, LGI1. We show that ADAM22, transmembrane protein when mutated itself seizure, serves as receptor for LGI1 enhances AMPA receptor-mediated hippocampal slices. The fails to bind ADAM22. ADAM22 anchored postsynaptic...
3D computer-generated holography uses a digital phase mask to shape the wavefront of laser beam into user-specified intensity pattern. Algorithms take target as input and compute hologram that generates it. However, arbitrary patterns are generally infeasible, so solutions approximate often sub-optimal. Here, we propose new non-convex optimization algorithm computes holograms by minimizing custom cost function is tailored particular applications (e.g., lithography, neural photostimulation)...
Optical methods capable of manipulating neural activity with cellular resolution and millisecond precision in three dimensions will accelerate the pace neuroscience research. Existing approaches for targeting individual neurons, however, fall short these requirements. Here we present a new multiphoton photo-excitation method, termed three-dimensional scanless holographic optogenetics temporal focusing (3D-SHOT), which allows precise, simultaneous photo-activation arbitrary sets neurons...
Understanding the mechanisms of perception, cognition, and behavior requires instruments that are capable recording controlling electrical activity many neurons simultaneously at high speeds. All-optical approaches particularly promising since they minimally invasive potentially scalable to experiments interrogating thousands or millions neurons. Conventional light-field microscopy provides a single-shot 3D fluorescence capture method with good light efficiency fast speed, but suffers from...
Significance Ultrasound stimulation modulates the electrical activity of excitable cells, including in neurons brain and central nervous system. Compared to other neuromodulatory techniques, ultrasound offers several advantages; for example, it can be noninvasively transmitted through skull focused deep regions. However, molecular basis underlying effects on neural is not known. Here, we show that activates mechanosensitive ion channel TRAAK membrane a manner analogous canonical mechanical...
Abstract Recurrent cortical activity sculpts visual perception by refining, amplifying or suppressing input. However, the rules that govern influence of recurrent remain enigmatic. We used ensemble-specific two-photon optogenetics in mouse cortex to isolate impact from external found spatial arrangement and feature preference stimulated ensemble neighboring neurons jointly determine net effect activity. Photoactivation these ensembles drives suppression all cells beyond 30 µm but uniformly...
Long-term potentiation (LTP) at hippocampal synapses is thought to involve the insertion of AMPA receptors into postsynaptic membrane. Conflicting evidence exists as whether calcium-permeable are inserted during LTP and synaptic activity mediated by newly required maintain increase in strength. Here, we rigorously test these hypotheses conclude that not nor does require ongoing be maintained.
Interneurons are born in subcortical germinative zones and tangentially migrate multiple streams above below the developing cortex, then, at appropriate developmental stage, radially into cortex. The factors that control formation of timing exit from remain obscure; moreover, rationale for this complicated plan is unclear. We show a chemokine, Cxcl12, an attractant interneurons during stage stream tangential migration. Furthermore, migratory accompanies loss responsiveness to Cxcl12 as...
How the billions of synapses in adult mammalian brain are precisely specified remains one fundamental questions neuroscience. Although a genetic program is likely to encode basic neural blueprint, much evidence suggests that experience-driven activity through NMDA receptors wires up neuronal circuits by inducing process similar long-term potentiation. To test this notion directly, we eliminated before and during synaptogenesis single cells vitro vivo. prevailing model would predict receptor...
The neocortex is functionally organized into layers. Layer four receives the densest bottom up sensory inputs, while layers 2/3 and 5 receive top down inputs that may convey predictive information. A subset of cortical somatostatin (SST) neurons, Martinotti cells, gate input by inhibiting apical dendrites pyramidal cells in 5, but it unknown whether an analogous inhibitory mechanism controls activity layer 4. Using high precision circuit mapping, vivo optogenetic perturbations, single cell...