A5056553250- Anaerobic Digestion and Biogas Production
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Single-cell and spatial transcriptomics
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Zebrafish Biomedical Research Applications
- Cancer Genomics and Diagnostics
- Wastewater Treatment and Nitrogen Removal
- Neuroscience and Neural Engineering
- Behavioral and Psychological Studies
- CRISPR and Genetic Engineering
- Animal Nutrition and Physiology
- Genomics and Phylogenetic Studies
- Developmental Biology and Gene Regulation
- Memory and Neural Mechanisms
- Kruppel-like factors research
- Genomics and Rare Diseases
- Genomic variations and chromosomal abnormalities
- Regulation of Appetite and Obesity
- Cannabis and Cannabinoid Research
- Insect and Pesticide Research
- RNA Research and Splicing
- Biofuel production and bioconversion
University of California, Berkeley
2008-2024
Invitae (United States)
2024
10X Genomics (United States)
2016-2018
Berkeley College
2009-2011
West Virginia State University
2007
University of Chicago
2004-2006
Louisiana State University Health Sciences Center Shreveport
1997-2004
National Institutes of Health
1999-2002
National Institute on Drug Abuse
1999-2002
National Institute of Diabetes and Digestive and Kidney Diseases
1999-2002
Large-scale population analyses coupled with advances in technology have demonstrated that the human genome is more diverse than originally thought. To date, this diversity has largely been uncovered using short-read whole-genome sequencing. However, these approaches fail to give a complete picture of genome. They struggle identify structural events, cannot access repetitive regions, and resolve into haplotypes. Here, we describe an approach retains long range information while maintaining...
The neocortex is functionally organized into layers. Layer four receives the densest bottom up sensory inputs, while layers 2/3 and 5 receive top down inputs that may convey predictive information. A subset of cortical somatostatin (SST) neurons, Martinotti cells, gate input by inhibiting apical dendrites pyramidal cells in 5, but it unknown whether an analogous inhibitory mechanism controls activity layer 4. Using high precision circuit mapping, vivo optogenetic perturbations, single cell...
The mammalian cortex is comprised of cells classified into types according to shared properties. Defining the contribution each cell type processes guided by essential for understanding its function in health and disease. We used transcriptomic epigenomic cortical taxonomies from mouse human define marker genes putative enhancers created a large toolkit transgenic lines enhancer AAVs selective targeting populations. report evaluation fifteen new driver lines, two reporter >800 different...
During vertebrate development, the endodermal germ layer becomes regionalized along its anteroposterior axis to give rise a variety of organs, including pancreas. Genetic studies in zebrafish and mice have established that signaling molecule retinoic acid (RA) plays crucial role endoderm patterning promotes pancreas development. To identify how RA signals pancreatic progenitors endoderm, we developed novel cell transplantation technique, using ability SOX32 transcription factor confer...
Illuminating the molecular identity and regulation of early progenitor cells in olfactory sensory epithelium represents an important challenge field neural development. We show both mouse zebrafish that winged helix transcription factor Foxg1 is expressed population placode. In mouse, first throughout placode but later becomes restricted to ventrolateral epithelium. The essential role development demonstrated by strikingly severe phenotype knock-out mice: older embryos have no recognizable...
Inductive signals from adjacent tissues initiate differentiation within the somite. In this study, we used mouse embryos mutant for BMP antagonists noggin (Nog) and gremlin 1 (Grem1) to characterize effects of signaling on specification sclerotome. We confirmed reduction Pax1 Pax9 expression in Nog mutants, but found that Nog;Grem1 double mutants completely fail sclerotome development. Furthermore, also lack one allele Grem1 exhibit a dramatic axial skeleton relative animals alone. By...
Summary: A heterotrophic bacterium has been isolated which can use thiocyanate as its sole source of cellular nitrogen and also sulphur; ammonium ions inhibit the utilization thiocyanate. It utilize both phenol simultaneously; it is a pseudomonad most similar to Pseudomonas stutzeri.
Abstract As the adoption and scope of genetic testing continue to expand, interpreting clinical significance DNA sequence variants at scale remains a formidable challenge, with high proportion classified as uncertain (VUSs). Genetic laboratories have historically relied, in part, on functional data from academic literature support variant classification. High-throughput assays or multiplex effect (MAVEs), designed assess effects protein stability function, represent an important increasingly...
Key pecking by pigeons was maintained arithmetic progressive‐ratio schedules of food delivery Successive conditions arranged different step sizes, and each condition remained in effect until behavior appeared stable. Each session continued a period time passed which no key pecks were recorded (the break‐point criterion); both 5‐min 15‐min criterion tested across range sizes. Average breaking points (i.e, the largest ratio completed) relatively unaffected step‐size magnitude, whereas average...
In our search for long-acting agents the treatment of cocaine abuse, a series optically pure hydroxylated derivatives 1-[2-[bis(4-fluorophenyl)methoxy]ethyl]-4-(3-phenylpropyl)piperazine (1) and 1-[2-(diphenylmethoxy)ethyl]-4-(3-phenylpropyl)piperazine (2) (GBR 12909 GBR 12935, respectively) were synthesized evaluated in vitro vivo. The enantiomers 2-hydroxylated analogues displayed substantial enantioselectivity. S higher dopamine transporter (DAT) affinity R found to interact at serotonin...
Summary. A novel device which could be used for the disruption of activated sludge flocs, using a transversely oscillating wire, is discussed. The acoustic microstreaming generated around wire produced hydrodynamic shear forces sufficiently strong to break up without causing loss in viability component cells. 20‐fold increase total heterotrophs and Thiobacillus ‐type organisms was observed. Electron micrographs provided visual evidence flocs.
The transcriptional mechanisms driving lineage specification during development are still largely unknown, as the interplay of multiple transcription factors makes it difficult to dissect these molecular events. Using a cell-based differentiation platform probe function, we investigated role key paraxial mesoderm and skeletal myogenic commitment factors—mesogenin 1 (Msgn1), T-box 6 (Tbx6), forkhead box C1 (Foxc1), paired 3 (Pax3), Paraxis, mesenchyme homeobox (Meox1), sine oculis-related...
S ummary . Activated sludge receiving ammonium thiocyanate (500 mg/l) was able to nitrify. The rate of ammonia oxidation decreased when >3 mg/l phenol (or cresols) added the sludge, and at 10 inhibited completely. Concentrations up 100 phenols did not affect nitrite oxidation. 2‐ 4‐methyl pyridine derivatives both
Dopaminergic projections exert widespread influence over multiple brain regions and modulate various behaviors including movement, reward learning, motivation. It is increasingly appreciated that dopamine neurons are heterogeneous in their gene expression, circuitry, physiology, function. Current approaches to target largely based on single drivers, which either label all or mark a subset but concurrently non-dopaminergic neurons. Here, we establish mouse line with Flpo recombinase expressed...
An investigation into the preparation of potential extended-release cocaine-abuse therapeutic agents afforded a series compounds related to 1-[2-(diphenylmethoxy)ethyl]-4-(3-phenylpropyl)piperazine (1a) and 1-[2-[bis(4-fluorophenyl)methoxy]ethyl]-4-(3-phenylpropyl)piperazine (1b) (GBR 12935 GBR 12909, respectively), which were designed, synthesized, evaluated for their ability bind dopamine transporter (DAT) inhibit uptake [(3)H]-labeled (DA). The addition hydroxy methoxy substituents...