Rachel McCue
A5049440195- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Immune cells in cancer
- Traumatic Brain Injury and Neurovascular Disturbances
- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Advanced Fluorescence Microscopy Techniques
- Pluripotent Stem Cells Research
- Dementia and Cognitive Impairment Research
- RNA Research and Splicing
- Virus-based gene therapy research
- Cell Image Analysis Techniques
- CRISPR and Genetic Engineering
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Cerebrospinal fluid and hydrocephalus
Allen Institute
2023-2024
Allen Institute for Brain Science
2023-2024
The mammalian brain consists of millions to billions cells that are organized into many cell types with specific spatial distribution patterns and structural functional properties1-3. Here we report a comprehensive high-resolution transcriptomic cell-type atlas for the whole adult mouse brain. was created by combining single-cell RNA-sequencing (scRNA-seq) dataset around 7 million profiled (approximately 4.0 passing quality control), approximately 4.3 using multiplexed error-robust...
The mammalian brain is composed of millions to billions cells that are organized into numerous cell types with specific spatial distribution patterns and structural functional properties. An essential step towards understanding function obtain a parts list, i.e., catalog types, the brain. Here, we report comprehensive high-resolution transcriptomic type atlas for whole adult mouse was created based on combination two single-cell-level, whole-brain-scale datasets: single-cell RNA-sequencing...
Alzheimer's disease (AD) is the leading cause of dementia in older adults. Although AD progression characterized by stereotyped accumulation proteinopathies, affected cellular populations remain understudied. Here we use multiomics, spatial genomics and reference atlases from BRAIN Initiative to study middle temporal gyrus cell types 84 donors with varying pathologies. This cohort includes 33 male 51 female donors, an average age at time death 88 years. We used quantitative neuropathology...
Alzheimer's disease (AD) is the most common cause of dementia in older adults. Neuropathological and imaging studies have demonstrated a progressive stereotyped accumulation protein aggregates, but underlying molecular cellular mechanisms driving AD progression vulnerable cell populations affected by remain coarsely understood. The current study harnesses single spatial genomics tools knowledge from BRAIN Initiative Cell Census Network to understand impact on middle temporal gyrus types. We...
Abstract Alzheimer’s disease (AD) is the most common cause of dementia in older adults. Neuropathological and imaging studies have demonstrated a progressive stereotyped accumulation protein aggregates, but underlying molecular cellular mechanisms driving AD progression vulnerable cell populations affected by remain coarsely understood. The current study harnesses single spatial genomics tools knowledge from BRAIN Initiative Cell Census Network to understand impact on middle temporal gyrus...
The mammalian cortex is comprised of cells classified into types according to shared properties. Defining the contribution each cell type processes guided by essential for understanding its function in health and disease. We used transcriptomic epigenomic cortical taxonomies from mouse human define marker genes putative enhancers created a large toolkit transgenic lines enhancer AAVs selective targeting populations. report evaluation fifteen new driver lines, two reporter >800 different...
The mammalian cortex is composed of a highly diverse set cell types and develops through series temporally regulated events that build out the type circuit foundation for cortical function. mechanisms underlying development different remain elusive. Single-cell transcriptomics provides capacity to systematically study across entire temporal range development. Here, we present comprehensive high-resolution transcriptomic epigenomic atlas developing mouse visual cortex. was built from...
The telencephalon of the mammalian brain comprises multiple regions and circuit pathways that play adaptive integrative roles in a variety functions. There is wide array GABAergic neurons telencephalon; they multitude functions, dysfunction these has been implicated diverse disorders. In this study, we conducted systematic in-depth analysis transcriptomic spatial organization neuronal types all mouse their developmental origins. This was accomplished by utilizing 611,423 single-cell...
We present an enhancer AAV toolbox for accessing and perturbing striatal cell types circuits. Best-in-class vectors were curated major neuron populations including medium spiny neurons (MSNs), direct indirect pathway MSNs, as well Sst-Chodl, Pvalb-Pthlh, cholinergic interneurons. Specificity was evaluated by multiple modes of molecular validation, three different routes virus delivery, with diverse transgene cargos. Importantly, we provide detailed information necessary to achieve reliable...