Bargavi Thyagarajan
A5085528673- HIV Research and Treatment
- RNA and protein synthesis mechanisms
- Influenza Virus Research Studies
- vaccines and immunoinformatics approaches
- HIV/AIDS Research and Interventions
- Neural dynamics and brain function
- Virus-based gene therapy research
- HIV, Drug Use, Sexual Risk
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Ethics in Clinical Research
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neural Engineering
- Pharmaceutical Quality and Counterfeiting
- Pharmaceutical Economics and Policy
- Genital Health and Disease
- Pluripotent Stem Cells Research
- Reproductive tract infections research
- Neurogenesis and neuroplasticity mechanisms
- RNA Research and Splicing
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- Neurogenetic and Muscular Disorders Research
Allen Institute
2022-2024
Allen Institute for Brain Science
2023-2024
International AIDS Society
2021
Global HIV Vaccine Enterprise
2017-2019
Cancer Research Center
2014
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2014
Fred Hutch Cancer Center
2014
Huntsman Cancer Institute
2010
University of Utah
2010
Influenza is notable for its evolutionary capacity to escape immunity targeting the viral hemagglutinin. We used deep mutational scanning examine extent which a high inherent tolerance contributes this antigenic evolvability. created mutant viruses that incorporate most of ≈104 amino-acid mutations hemagglutinin from A/WSN/1933 (H1N1) influenza. After passaging these in tissue culture select functional variants, we sequencing quantify mutation frequencies before and after selection. These...
Human cortex transcriptomic studies have revealed a hierarchical organization of γ-aminobutyric acid-producing (GABAergic) neurons from subclasses to high diversity more granular types. Rapid GABAergic neuron viral genetic labeling plus Patch-seq (patch-clamp electrophysiology single-cell RNA sequencing) sampling in human brain slices was used reliably target and analyze individual This characterization elucidated transitions between PVALB SST subclasses, morphological heterogeneity within...
Aging is a complex phenotype responsive to plethora of environmental inputs; yet only limited number transcriptional regulators are known influence life span. How the downstream expression programs mediated by these factors (or others) coordinated into common or distinct set aging effectors an addressable question in model organisms, such as C. elegans. Here, we establish transcription factor ETS-4, ortholog vertebrate SPDEF, longevity determinant. Adult worms with ets-4 mutations had...
Abstract Proper brain function requires the assembly and of diverse populations neurons glia. Single cell gene expression studies have mostly focused on characterization neuronal diversity; however, recent revealed substantial diversity glial cells, particularly astrocytes. To better understand types their roles in neurobiology, we built a new suite adeno-associated viral (AAV)-based genetic tools to enable access astrocytes oligodendrocytes. These oligodendrocyte astrocyte enhancer-AAVs are...
We present an enhancer AAV toolbox for accessing and perturbing striatal cell types circuits. Best-in-class vectors were curated major neuron populations including medium spiny neurons (MSNs), direct indirect pathway MSNs, as well Sst-Chodl, Pvalb-Pthlh, cholinergic interneurons. Specificity was evaluated by multiple modes of molecular validation, three different routes virus delivery, with diverse transgene cargos. Importantly, we provide detailed information necessary to achieve reliable...
Abstract Human cortical interneurons have been challenging to study due high diversity and lack of mature brain tissue platforms genetic targeting tools. We employed rapid GABAergic neuron viral labeling plus unbiased Patch-seq sampling in slices define the signature morpho-electric properties neurons human neocortex. Viral greatly facilitated SST subclass, including primate specialized double bouquet cells which mapped two transcriptomic types. Multimodal analysis uncovered an type with...
Summary Experimental access to cell types within the mammalian spinal cord is severely limited by availability of genetic tools. To enable lower motor neurons (LMNs) and LMN subtypes, which function integrate information from brain control movement through direct innervation effector muscles, we generated single multiome datasets mouse macaque cords discovered putative enhancers for each neuronal population. We cloned these into adeno-associated viral vectors (AAVs) driving a reporter...
Abstract Influenza is notable for its evolutionary capacity to escape immunity targeting the viral hemagglutinin. We used deep mutational scanning examine extent which a high inherent tolerance contributes this antigenic evolvability. created mutant viruses that incorporate most of ≈ 10 4 amino-acid mutations hemagglutinin from A/WSN/1933 (H1N1) influenza. After passaging these in tissue culture select functional variants, we sequencing quantify mutation frequencies before and after...
The HIV Research for Prevention (HIVR4P) conference is dedicated to advancing prevention research, responding a growing consensus that effective and durable will require combination of approaches as well unprecedented collaboration among scientists, practitioners, community workers from different fields geographic areas. theme in 2018, "From Impact," acknowledged an increasing focus on translation promising research findings into practical, accessible, affordable options those who need them...
HIV Research for Prevention: AIDS Vaccine, Microbicide, and ARV-based Prevention Science (HIVR4P) was built on a growing consensus that effective prevention requires combination of approaches understanding, analyzing, debating the cross-cutting issues impact research are all essential to combat global HIV/AIDS epidemic. To end, biennial HIVR4P conference is dedicated biomedical approaches, including vaccines, microbicides, pre-exposure prophylaxis, treatment as prevention. The 2016 held in...
The HIV Research for Prevention (HIVR4P) conference catalyzes knowledge sharing on biomedical prevention interventions such as vaccines, antibody infusions, pre-exposure prophylaxis, and microbicides in totality-from the molecular details delivery formulations to behavioral, social, structural underpinnings. HIVR4P // Virtual was held over course of 2 weeks January 27-28 February 3-4, 2021 coronavirus disease 2019 (COVID-19) pandemic continued inflict unprecedented harm globally. community...