Deja Monet
A5033438527- Neuroinflammation and Neurodegeneration Mechanisms
- Neural dynamics and brain function
- RNA regulation and disease
- Virus-based gene therapy research
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
Allen Institute
2023
Allen Institute for Brain Science
2023
Abstract Delivering genes to and across the brain vasculature efficiently specifically species remains a critical challenge for addressing neurological diseases. We have evolved adeno-associated virus (AAV9) capsids into vectors that transduce endothelial cells following systemic administration in wild-type mice with diverse genetic backgrounds, rats. These AAVs also exhibit superior transduction of CNS non-human primates (marmosets rhesus macaques), ex vivo human slices, although tropism is...
Rodent studies have demonstrated that synaptic dynamics from excitatory to inhibitory neuron types are often dependent on the target cell type. However, these cell-specific properties not been well investigated in human cortex, where there major technical challenges reliably obtaining healthy tissue, conducting multiple patch-clamp recordings types, and identifying those types. Here, we take advantage of newly developed methods for neurosurgical tissue analysis with recordings,
Abstract Proper brain function requires the assembly and of diverse populations neurons glia. Single cell gene expression studies have mostly focused on characterization neuronal diversity; however, recent revealed substantial diversity glial cells, particularly astrocytes. To better understand types their roles in neurobiology, we built a new suite adeno-associated viral (AAV)-based genetic tools to enable access astrocytes oligodendrocytes. These oligodendrocyte astrocyte enhancer-AAVs are...
Delivering genes to and across the brain vasculature efficiently specifically species remains a critical challenge for addressing neurological diseases. We have evolved adeno-associated virus (AAV9) capsids into vectors that transduce endothelial cells following systemic administration in wild-type mice with diverse genetic backgrounds rats. These AAVs also exhibit superior transduction of CNS non-human primates (marmosets rhesus macaques),