A5028572760- Optical Imaging and Spectroscopy Techniques
- Neuroscience and Neural Engineering
- Photoacoustic and Ultrasonic Imaging
- Neuroinflammation and Neurodegeneration Mechanisms
- Functional Brain Connectivity Studies
- Heart Rate Variability and Autonomic Control
- Retinal Development and Disorders
- Advanced Fluorescence Microscopy Techniques
- Neural dynamics and brain function
- Optical Coherence Tomography Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Thermoregulation and physiological responses
- Vagus Nerve Stimulation Research
- Infrared Thermography in Medicine
- Analytical Chemistry and Sensors
- Neuroscience and Neuropharmacology Research
- Climate Change and Health Impacts
- Photoreceptor and optogenetics research
- Retinal Diseases and Treatments
- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Olfactory and Sensory Function Studies
- Advanced Computing and Algorithms
- Ultrasound Imaging and Elastography
- Speech and Audio Processing
Allen Institute
2023-2024
Allen Institute for Neural Dynamics
2023-2024
North Seattle College
2024
Boston University
2016-2023
Louis Stokes Cleveland VA Medical Center
2013-2018
Case Western Reserve University
2013-2018
Sardar Vallabhbhai National Institute of Technology Surat
2016
University School
2014
Chronic cranial windows allow for longitudinal brain imaging experiments in awake, behaving mice. Different technologies have their unique advantages and combining multiple modalities offers measurements of a wide spectrum neuronal, glial, vascular, metabolic parameters needed comprehensive investigation physiological pathophysiological mechanisms. Here, we detail suite surgical techniques installation different targeted specific combination. Following these practices will yield higher...
Animal models of stroke are used extensively to study the mechanisms involved in acute and chronic phases recovery following stroke. A translatable animal model that closely mimics a human is essential understanding processes as well developing therapies improve functional outcomes. We describe photothrombosis capable targeting single distal pial branch middle cerebral artery with minimal damage surrounding parenchyma awake head-fixed mice. Mice implanted cranial windows above one hemisphere...
Abstract Laser speckle contrast imaging (LSCI) is a rapidly developing technology broadly applied for the full-field characterization of tissue perfusion. Over recent years, significant advancements have been made in interpreting LSCI measurements and improving technique’s accuracy. On other hand, method’s precision has yet to be studied detail, despite being as important accuracy many biomedical applications. Here we combine simulation, theory animal experiments systematically evaluate...
Abstract Proper brain function requires the assembly and of diverse populations neurons glia. Single cell gene expression studies have mostly focused on characterization neuronal diversity; however, recent revealed substantial diversity glial cells, particularly astrocytes. To better understand types their roles in neurobiology, we built a new suite adeno-associated viral (AAV)-based genetic tools to enable access astrocytes oligodendrocytes. These oligodendrocyte astrocyte enhancer-AAVs are...
Functional neuroimaging, which measures hemodynamic responses to brain activity, has great potential for monitoring recovery in stroke patients and guiding rehabilitation during recovery. However, after are almost always altered relative healthy subjects it is still unclear if these alterations reflect the underlying physiology or purely due vascular injury. In other words, we do not know effect of on neurovascular coupling therefore limited our ability use functional neuroimaging accurately...
Optical coherence tomography angiography (OCTA) has been widely used for en face visualization of the microvasculature, but is challenged real three-dimensional (3-D) topologic imaging due to "tail" artifacts that appear below large vessels. Further, OCTA generally incapable differentiating descending arterioles from ascending venules. We introduce a normalized field autocorrelation function-based (g1-OCTA), which minimizes tail and capable distinguishing penetrating venules in 3-D image. g1...
We have recently demonstrated that partial inhibition of the cluster differentiation 14 (CD14) innate immunity co-receptor pathway improves long-term performance intracortical microelectrodes better than complete inhibition. hypothesized activation CD14 was critical to a neuroprotective response injury associated with initial and sustained device implantation. Therefore, here we investigated role two receptors closely interact in inflammatory activation. implanted silicon planar...
Significance: The optical properties of biological samples provide information about the structural characteristics tissue and any changes arising from pathological conditions. Optical coherence tomography (OCT) has proven to be capable extracting tissue's using a model that combines exponential decay due scattering axial point spread function arises confocal nature detection system, particularly for higher numerical aperture (NA) measurements. A weakness in estimating is inter-parameter...
SignificanceGenetically encoded calcium ion (Ca2+) indicators (GECIs) are powerful tools for monitoring intracellular Ca2+ concentration changes in living cells and model organisms. In particular, GECIs have found particular utility the transient increase of that is associated with neuronal action potential. However, palette highly optimized imaging activity remains relatively limited. Expanding selection available to include new colors distinct photophysical properties could create...
Abstract Utilization of functional ultrasound (fUS) in cerebral vascular imaging is gaining popularity among neuroscientists. In this article, we describe a chronic surgical preparation method that allows longitudinal studies and therefore applicable to wide range studies, especially on aging, stroke, neurodegenerative diseases. This can also be used with awake mice; hence, the deleterious effects anesthesia neurovascular responses avoided. addition fUS imaging, researchers take advantage...
Ischemic stroke leads to a reduction or complete loss of blood supply causing injury brain tissue, which ultimately behavioral impairment. Optical techniques are widely used study the structural and functional changes that result as consequence ischemic both in acute chronic phases recovery. It is currently challenge accurately estimate spatial extent infarct without use histological parameters however, order follow recovery mechanisms longitudinally at mesoscopic scale it essential know...
Behavioral neuroscience studies in freely moving rodents require small, light-weight implants to facilitate neural recording and stimulation. Our goal was develop an integrated package of 3D printed parts assembly aids for labs rapidly fabricate, with minimal training, implant that combines individually positionable microelectrodes, optical fiber, zero insertion force (ZIF-clip) headstage connection, secondary electrodes, e.g. electromyography (EMG).Starting from previous designs position...
ABSTRACT Laser speckle contrast imaging (LSCI) is a technique broadly applied in research and clinical settings for full-field characterization of tissue perfusion. It based on the analysis pattern contrast, which can be theoretically related to decorrelation time - quantitative measure dynamics. A direct conversion, however, requires prior knowledge specific parameters optical system scattering media thus often impractical. For this reason, because nature some most common applications, LSCI...
Human uses visual information while trying to understand speech, especially in noisy conditions or situations where the audio signal is not available.Lip reading technique of a comprehensive understanding underlying speech by processing on movement lips.However, recognition lip motion difficult task since region interest (ROI) nonlinear and noisy.In proposed method system we have used two stage feature extraction model which precised, discriminative computation efficient.The first 3D...
Abstract Functional neuroimaging, which measures hemodynamic responses to brain activity, has great potential for monitoring stroke patients. However, the neurophysiological interpretations of these signals remain a challenge as is likely alter both neural activity and neurovascular coupling. To address this challenge, we simultaneously captured through fluorescence calcium imaging, hemodynamics, intrinsic optical signal during longitudinal recovery. We found that photothrombotic...
ABSTRACT Significance Genetically encoded calcium ion (Ca 2+ ) indicators (GECIs) are powerful tools for monitoring intracellular Ca concentration changes in living cells and model organisms. In particular, GECIs have found particular utility the transient increase of that is associated with neuronal action potential. However, palette highly optimized imaging activity remains relatively limited. Expanding selection available to include new colors distinct photophysical properties could...
By obtaining structural and functional information such as changes in tissue properties, cerebral blood flow, hemodynamics through optical imaging, we aim to better interpret human MRI data guide therapeutic interventions following stroke.
Stroke is one of the leading causes long-term disability worldwide. While most stroke survivors experience some degree spontaneous recovery over time, highly variable among individuals and rehabilitation primary treatment to improve recovery. Functional neuroimaging, which measures hemodynamic responses brain activity, has great potential for monitoring in patients guiding rehabilitation. However, after are almost always altered relative healthy subjects it still unclear if these alterations...
We present the use of intrinsic optical signal imaging to monitor progression ischemic stroke in acute and chronic phases recovery. An optimized mouse photothrombosis model is used induce sensory forelimb area longitudinal recovery assessed using resting state functional connectivity as well stimulation. These measures are then correlated with behavioral outcome a asymmetry test. Obtaining both will enable better interpretation human neuroimaging studies allow their understanding stroke.