A5079698642- Immune Cell Function and Interaction
- HIV Research and Treatment
- Sphingolipid Metabolism and Signaling
- T-cell and B-cell Immunology
- Immune cells in cancer
- Reproductive System and Pregnancy
- Chronic Lymphocytic Leukemia Research
- HIV/AIDS drug development and treatment
- Blood donation and transfusion practices
- Phagocytosis and Immune Regulation
- Monoclonal and Polyclonal Antibodies Research
- Hemoglobinopathies and Related Disorders
- Platelet Disorders and Treatments
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Systemic Lupus Erythematosus Research
- HIV/AIDS Research and Interventions
- HIV, Drug Use, Sexual Risk
- Iron Metabolism and Disorders
University of Zambia
2024
University of California, Los Angeles
2015-2023
Black AIDS Institute
2016-2023
George Washington University
2018-2022
APLA Health
2015
Laboratory of Molecular Genetics
2015
Olive View-UCLA Medical Center
2015
Abstract Although CD31 expression on human thymocytes has been reported, a detailed analysis of at various stages T cell development in the thymus is missing. In this study, we provide global picture evolution from CD34+ hematopoietic precursor to CD45RA+ mature CD4+ and CD8+ single-positive (SP) cells. Using nine-color flow cytometry, show that highly expressed progenitors stays high until early double-positive stage (CD3−CD4+CD8α+β−). After β-selection, levels become low undetectable. then...
Primary cell models of human immunodeficiency virus (HIV) latency have become tools to both understand the mechanisms involved in establishment and test preclinical strategies toward HIV-1 cure. These rely on infection CD4 T cells from healthy donors. As such, these provide an opportunity explore role biological sex, age, HIV status reactivation latent vitro. We used established primary model based generation latently infected central memory with CXCR4 strain HIV-1NL4-3 address whether...
“Shock and kill” therapeutic strategies toward HIV eradication are based on the transcriptional activation of latent with a latency-reversing agent (LRA) consequent killing reactivated cell by either cytopathic effect or an arm immune system. We have recently found several benzotriazole benzotriazine analogues that ability to reactivate inhibiting signal transducer activator transcription 5 (STAT5) SUMOylation promoting STAT5 binding long terminal repeat increasing its activity.
Antiretroviral drugs that target various stages of the Human Immunodeficiency Virus (HIV) life cycle have been effective in curbing AIDS epidemic. However, drug resistance, off-target effects antiretroviral therapy (ART), and varying efficacy prevention underscore need to develop novel alternative therapeutics. In this study, we investigated whether targeting signaling molecule Sphingosine-1-phosphate (S1P) would inhibit HIV-1 infection generation latent reservoir primary CD4 T cells. We...
Sphingosine-1-phosphate (S1P) is a sphingolipid modulator of myriad cellular processes, and therapeutic targeting S1P signaling utilized clinically to treat multiple sclerosis. We have previously shown that functional antagonism receptors reduces cell-free, cell-to-cell, latent HIV-1 infection in primary CD4 T cells. In this work, we examined whether sphingosine kinase 1 or 2 (SPHK1/2) inhibit production would prevent using isolates infectious molecular clones. SPHK inhibition reduced HIV...
Emigration of mature naive CD4 SP T cells from the human thymus to periphery is not fully understood, although elucidation mechanisms that govern egress crucial understanding both basic immunology and immune response in diseases such as HIV infection. Recent work has brought light requirement for sphingosine-1-phosphate (S1P) its receptors a variety fields including T-cell mice. We are examining expression function this novel requisite receptor within thymus, characterizing changes observed...
Humoral immune response is important in fighting pathogens by the production of specific antibodies B cells. In germinal centers, T follicular helper (TFH) cells provide help to B-cell antibody but also contribute HIV persistence. regulatory (TFR) cells, which inhibit function TFH express similar surface markers. Since FOXP3 only marker that distinguishes TFR from it unknown whether increase observed infection and persistence may be partly due an Using multicolor flow cytometry detect...
ABSTRACT Background Anemia affects one in three pregnant women worldwide, with the greatest burden South Asia and sub-Saharan Africa. During pregnancy, anemia has been linked to an increased risk of adverse maternal neonatal health outcomes. Despite widespread recognition that can complicate critical gaps persist our understanding specific causes cutoffs used diagnose each trimester postpartum period. Methods analysis The Redefining Maternal Pregnancy Postpartum (ReMAPP) study is a...
The HIV Research for Prevention (HIVR4P) conference catalyzes knowledge sharing on biomedical prevention interventions such as vaccines, antibody infusions, pre-exposure prophylaxis, and microbicides in totality-from the molecular details delivery formulations to behavioral, social, structural underpinnings. HIVR4P // Virtual was held over course of 2 weeks January 27-28 February 3-4, 2021 coronavirus disease 2019 (COVID-19) pandemic continued inflict unprecedented harm globally. community...
Abstract Sphingosine-1-phosphate receptor 1 (S1P-R1) is essential in mice for homing of lymphoid cells as well egress mature thymocytes from the thymus to periphery. We have recently demonstrated that human thymus, response S1P-R1 its ligand, (S1P), required Herein we demonstrate immune dysregulation accompanying Human Immunodeficiency Virus (HIV) infection alters expression on thymocytes, increasing at both mRNA and protein levels. Moreover, function maintained, assayed by flow cytometry...
Abstract Although CD31 expression on human thymocytes has been reported, a detailed analysis of at various stages T cell development in the thymus is missing. Here we address evolution from CD34+ hematopoietic precursor to CD45RA+ mature CD4+ and CD8+ single positive (SP) cells. Using 9-color flow cytometry, show that highly expressed progenitors stays high until early double stage (CD3- CD8α+β-) when levels become low undetectable. then increases peaks CD3high CD4+CD8+ thymocytes. However,...
Regeneration of functional naïve T lymphocytes following the onset human immunodeficiency virus (HIV) infection remains a crucial issue for people living with HIV (PLWH), even when adhering to antiretroviral therapy (ART). Thus far, reports on impact HIV-1 entry thymic precursors and egress from thymus are limited. We examined Sphingosine-1-phosphate (S1P) signaling, which governs thymocytes periphery. Using in vitro experiments primary vivo ex studies humanized mice, we show that results...
Abstract Lack of adequate T cell regeneration in HIV infected individuals is likely due to a defect the entry hematopoietic stem cells (HSC) into and egress naïve from thymus periphery. We studied effect HIV-1 infection on receptors Sphingosine-1-phosphate (S1P), chemotactic sphingolipid mediator, during thymocyte trafficking. Our novel findings show that changes expression patterns S1P-R1 thymocytes about exit thymus. To examine dynamics HSC progenitors infection, mice implanted with human...
Abstract Regeneration of functional naïve T lymphocytes following the onset infection remains a crucial issue for HIV + individuals, even while adhering to Antiretroviral Therapy (ART). Thus far, reports on impact HIV-1 entry thymic precursors and egress are limited. Therefore, we examined Sphingosine-1-phosphate (S1P) signaling, which governs thymocytes from thymus periphery. Using in vitro experiments with primary human vivo ex studies humanized mice, demonstrate that upregulates...