A5069883599- Genetics, Aging, and Longevity in Model Organisms
- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Mitochondrial Function and Pathology
- Circadian rhythm and melatonin
- Biochemical Acid Research Studies
- Birth, Development, and Health
- Ferroptosis and cancer prognosis
- Cholesterol and Lipid Metabolism
- CRISPR and Genetic Engineering
- Cancer, Lipids, and Metabolism
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Eicosanoids and Hypertension Pharmacology
- Microbial Metabolic Engineering and Bioproduction
- Hippo pathway signaling and YAP/TAZ
- Genomics, phytochemicals, and oxidative stress
- Fatty Acid Research and Health
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Primary Care and Health Outcomes
- Healthcare Policy and Management
- RNA Interference and Gene Delivery
- GDF15 and Related Biomarkers
- Infant Development and Preterm Care
Washington State University
2016-2025
Michigan State University
2018-2022
University of Missouri–Kansas City
2015-2021
Children's Mercy Hospital
2015-2021
Truman Medical Center
2015
Cornell University
1996-2000
Royal Hospital for Children
1971
Polyunsaturated fatty acids (PUFAs) are important membrane components and precursors of signaling molecules. To investigate the roles these in growth, development, neurological function an animal system, we isolated Caenorhabditis elegans mutants deficient PUFA synthesis by direct analysis acid composition. C. possesses all desaturase elongase activities to synthesize arachidonic eicosapentaenoic from saturated precursors. In our screen identified with defects each desaturation elongation...
The sterol regulatory element-binding protein (SREBP) transcription factor family is a critical regulator of lipid and homeostasis in eukaryotes. In mammals, SREBPs are highly active the fed state to promote expression lipogenic cholesterogenic genes facilitate fat storage. During fasting, SREBP-dependent lipid/cholesterol synthesis rapidly diminished mouse liver; however, mechanism has remained incompletely understood. Moreover, evolutionary conservation fasting regulation programs gene...
The nematode Caenorhabditis elegans has emerged as an important model for studies of the regulation fat storage. C. feed on bacteria, and various strains E. coli are commonly used in research settings. However, it is not known whether particular bacterial diets affect storage metabolism.Fat staining fixed nematodes, well biochemical analysis lipid classes, revealed considerable differences stores growing four different strains. Fatty acid composition carbohydrate levels differ examined these...
ABSTRACT The generation of asymmetry in the one-cell embryo Caenorhabditis elegans is necessary to establish anterior-posterior axis and ensure proper identity early blastomeres. Maternal-effect lethal mutations with a partitioning defective phenotype (par) have identified several genes involved this process. We new gene, par-6, which acts conjunction other par properly localize cytoplasmic components embryo. phenotypes par-6 embryos include equal-sized blastomeres, improper localization P...
ABSTRACT During the first cell cycle of Caenorhabditis elegans embryogenesis, asymmetries are established that essential for determining subsequent developmental fates daughter cells. The maternally expressed par genes required establishing this polarity. products several have been found to be themselves asymmetrically distributed in cycle. We identified par-4 gene C. elegans, and find it encodes a putative serine-threonine kinase with similarity human associated Peutz-Jeghers Syndrome, LKB1...
Delta-9 desaturases, also known as stearoyl-CoA are lipogenic enzymes responsible for the generation of vital components membranes and energy storage molecules. We have identified a novel nuclear hormone receptor, NHR-80, that regulates delta-9 desaturase gene expression in Caenorhabditis elegans. Here we describe fatty acid compositions, lifespans, studies strains carrying mutations nhr-80 three genes encoding fat-5, fat-6, fat-7. The single mutants display only subtle changes composition...
Monounsaturated fatty acids are essential components of membrane and storage lipids. Their synthesis depends on the conversion saturated to unsaturated by Delta9 desaturases. Caenorhabditis elegans has three desaturases encoded genes fat-5, fat-6, fat-7. We generated nematodes that display a range altered acid compositions constructing double-mutant strains combine mutations in All combinations have reduced survival at low temperatures. The fat-5;fat-6 double mutants relatively subtle...
Significance The unfolded protein response of the endoplasmic reticulum (UPR ER ) is induced by proteotoxic conditions. Lipid disequilibrium also activates UPR , but whether this activation accompanied disturbed proteostasis in vivo remains controversial. In study, we show that nematode Caenorhabditis elegans compromised fatty acid desaturation and reduced phosphatidylcholine production activate without overt proteostatic imbalance, as assessed molecular, pharmacological, genetic analyses....
Fatty acid desaturation regulates membrane function and fat storage in animals. To determine the contribution of stearoyl-CoA desaturase (SCD) activity on development nematode Caenorhabditis elegans, we analyzed lipid composition droplet size fat-6;fat-7 mutants independently combination with disrupted conserved metabolic pathways. C. elegans impaired SCD displayed both reduced stores decreased size. Mutants daf-2 (insulin-like growth factor receptor), rsks-1 (homolog p70S6 kinase, an...
Caenorhabditis elegans gut granules are intestine specific lysosome-related organelles with birefringent and autofluorescent contents. We identified pgp-2, which encodes an ABC transporter, in screens for genes required the proper formation of granules. pgp-2(-) embryos mislocalize material into intestinal lumen lacking acidified V-ATPase-containing compartments. Adults without pgp-2(+) function similarly lack granule characteristics. These cellular phenotypes indicate that animals defective...
A growing body of research indicates that amyotrophic lateral sclerosis (ALS) patients and mouse models ALS exhibit metabolic dysfunction. subpopulation possesses higher levels resting energy expenditure lower fat-free mass compared to healthy controls. Similarly, two mutant copper zinc superoxide dismutase 1 (mSOD1) familial possess a hypermetabolic phenotype. The pathophysiological relevance the bioenergetic defects observed in remains largely elusive. AMP-activated protein kinase (AMPK)...