A5049886131- Magnetic properties of thin films
- Aquaculture disease management and microbiota
- Bacterial Genetics and Biotechnology
- Aquaculture Nutrition and Growth
- Bacteriophages and microbial interactions
- Magnetic Properties and Applications
- Theoretical and Computational Physics
- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Magneto-Optical Properties and Applications
- Microbial infections and disease research
- Bacterial biofilms and quorum sensing
- Trace Elements in Health
- Plant Pathogenic Bacteria Studies
- Invertebrate Immune Response Mechanisms
- Advanced biosensing and bioanalysis techniques
- Marine animal studies overview
- Immune cells in cancer
- Physics of Superconductivity and Magnetism
- DNA and Nucleic Acid Chemistry
- ZnO doping and properties
- Plant Virus Research Studies
- RNA and protein synthesis mechanisms
- Magnetic and transport properties of perovskites and related materials
- Genomics and Phylogenetic Studies
Seoul National University
2020-2025
Cornell University
2015-2024
Harvard University
2022-2024
Howard Hughes Medical Institute
2022-2023
New Generation University College
2020
Chung-Ang University
2008-2011
Pusan National University
2009
Massachusetts Institute of Technology
2004-2008
IIT@MIT
2008
IBM Research - Almaden
2008
The mammalian brain consists of millions to billions cells that are organized into many cell types with specific spatial distribution patterns and structural functional properties1-3. Here we report a comprehensive high-resolution transcriptomic cell-type atlas for the whole adult mouse brain. was created by combining single-cell RNA-sequencing (scRNA-seq) dataset around 7 million profiled (approximately 4.0 passing quality control), approximately 4.3 using multiplexed error-robust...
In mammalian brains, millions to billions of cells form complex interaction networks enable a wide range functions. The enormous diversity and intricate organization have impeded our understanding the molecular cellular basis brain function. Recent advances in spatially resolved single-cell transcriptomics enabled systematic mapping spatial molecularly defined cell types tissues1-3, including several regions (for example, refs. 1-11). However, comprehensive atlas whole is still missing. Here...
The mammalian brain is composed of millions to billions cells that are organized into numerous cell types with specific spatial distribution patterns and structural functional properties. An essential step towards understanding function obtain a parts list, i.e., catalog types, the brain. Here, we report comprehensive high-resolution transcriptomic type atlas for whole adult mouse was created based on combination two single-cell-level, whole-brain-scale datasets: single-cell RNA-sequencing...
Abstract Binding and unbinding of transcription regulators at operator sites constitute a primary mechanism for gene regulation. While many cellular factors are known to regulate their binding, little is on how cells can modulate Using nanometer-precision single-molecule tracking, we study the kinetics from DNA two metal-sensing in living Escherichia coli cells. We find that they show unusual concentration-dependent chromosomal recognition both apo holo forms. Unexpectedly, further varies...
In mammalian brains, tens of millions to billions cells form complex interaction networks enable a wide range functions. The enormous diversity and intricate organization in the brain have so far hindered our understanding molecular cellular basis its Recent advances spatially resolved single-cell transcriptomics allowed systematic mapping spatial molecularly defined cell types tissues1-3. However, these approaches only been applied few regions1-11 comprehensive atlas whole is still missing....
Curcumin exhibited numerous key activities including antioxidant, anti-inflammatory, and immunostimulatory effects in fish. This study evaluated the protective of curcumin (CUR) against lead (Pb)-induced toxicities Healthy Cyprinus carpio L. individuals were segregated into control, Pb only, Pb+CUR, CUR only groups. groups exposed to 1 mg L −1 Pb, fed a basal diet supplemented with 15 g kg−1 CUR. After eight weeks, growth performance, accumulation tissues, various haemato-biochemical...
Summary The periaqueductal gray (PAG) is a critical midbrain hub that relays information from the forebrain to motor and autonomic brainstem centers orchestrate instinctive behaviors. current organization of PAG into four main radial columns lacks resolution needed account for vast range functions. Using spatially resolved single-cell transcriptomic measurements, we uncovered widespread transcriptional heterogeneity in with >100 excitatory inhibitory neuronal populations, which further...
Narrow mesoscopic NiFe/Cu/Co elliptical rings exhibit room-temperature giant magnetoresistance with distinct resistance levels corresponding to three different micromagnetic states. The highest and lowest states of the multilayer correspond Co layer being in a bidomain state, antiparallel or parallel, respectively, NiFe, while intermediate corresponds vortex state. Micromagnetic simulations suggest that behavior these is dominated by magnetostatic interactions between domain walls NiFe...
The remanent states and hysteretic behavior of thin-film magnetic rings has been investigated experimentally by micromagnetic modeling. Rings diameters 160--520 nm, made from Co using lift-off processing, show three distinct states: a vortex state, an ``onion'' state with two head-on walls, ``twisted'' containing $360\ifmmode^\circ\else\textdegree\fi{}$ wall. range stability these varies ring geometry, smaller width showing higher switching fields greater variability.
A series of two dimensional close-packed $\mathrm{Co}$, $\mathrm{NiFe}$, and $\mathrm{CoFe}∕\mathrm{Cu}∕\mathrm{NiFe}$ magnetic particle arrays, in which the particles have mean diameters $34\phantom{\rule{0.3em}{0ex}}\mathrm{nm}$, thicknesses $5--20\phantom{\rule{0.3em}{0ex}}\mathrm{nm}$, periodicity $56\phantom{\rule{0.3em}{0ex}}\mathrm{nm}$, were made using a process based on self-assembled polystyrene-$b$-polyferrocenyldimethylsilane block copolymer templates. Interparticle magnetostatic...
Significance MerR-family regulators act on suboptimal promoters to control the transcriptions of genes that help bacteria defend against a diverse set metals and drugs. How they modulate RNA polymerase (RNAP) activity transcription initiation remains unclear, however. Here we show CueR—a Cu + -responsive metalloregulator—biases kinetic sampling RNAP binding events lead two noninterconverting states: dead-end complex repress or an open activate transcription, constituting branched pathway...
Abstract Newly evolving strains of pathogenic microorganisms in aquaculture are posing formidable challenge controlling and/or eradication diseases affecting aquaculture. Many research studies have aimed to address this challenge, and the delivery vaccines using biocompatible nano‐delivery systems is widely investigated. Nanoparticles (NPs) tremendous potential for application as vehicles novel vaccines. At present, various polymeric non‐polymeric NPs utilized deliver aquatic species. This...
Abstract Background Antibiotic-resistant bacteria have emerged as a serious problem; bacteriophages have, therefore, been proposed therapeutic alternative to antibiotics. Several authorities, such pharmacopeia, FDA, confirmed their safety, and some are commercially available worldwide. The demand for is expected increase exponentially in the future; hence, there an urgent need mass-produce economically. Unlike replication of non-lytic bacteriophages, lytic replicated by lysing host bacteria,...
The flux-closed or "vortex" state in thin-film magnetic rings has been proposed as a data storage token, but it proven difficult to control the vortex chirality simple manner. Here, model is described that predicts of an elliptical ring function direction applied field and exchange bias, based on change energy system domain walls move. Experimental measurements Co Co/IrMn with 3.2 microm major axis are excellent agreement model. circulation can therefore be tailored appropriate combination...
Significance Multicomponent efflux pumps confer clinically relevant drug resistance in Gram-negative bacteria. These pumps, once assembled to function, traverse the periplasm linking inner and outer membranes tightly. However, little is known on how these can operate efficiently without compromising periplasmic plasticity. We show here that Escherichia coli , tripartite complex CusCBA for Cu + Ag exists as a dynamic structure shifts toward form response metal stress. Unexpectedly, adaptor...
Significance The field of mechanobiology examines how physical forces modulate cell physiology and has traditionally focused on eukaryotic organisms. Here we show that in bacteria, mechanical stresses can interrupt the structure function a molecular assembly used by Gram-negative bacteria to survive grow presence toxins. This work provides evidence like mammalian cells, respond through complexes at surface ways are relevant growth. Our observations further suggest may be synergistically with...
The recent outbreak of blight in pome fruit plants has been a major concern as there are two indistinguishable Erwinia species, amylovora and E. pyrifoliae, which cause South Korea. Although is strict management protocol consisting antibiotic-based prevention, the area number cases outbreaks have increased. In this study, we isolated four bacteriophages, pEp_SNUABM_03, 04, 11, 12, that infect both pyrifoliae evaluated their potential antimicrobial agents for administration against...
Since its introduction as a model organism in the 1980s, use of zebrafish (Danio rerio) research has expanded worldwide. Despite now widespread research, guidelines to safeguard ethical treatment zebrafish, particularly with regard euthanasia and humane endpoint practices, remain inadequate. One well-recognized example is excess tricaine methanesulfonate (MS-222) means euthanize regardless life stage. In this study, through nationwide expert elicitation, we provide detailed account practices...
Environmental DNA (eDNA) serves as a non-invasive tool for monitoring the presence of specific organisms in challenging or hard-to-access areas. We attempted Korean cetacean species by extracting eDNA from western and southern seas Republic Korea, well around Jeju Island. In present study, we focused on two representative cetaceans Sea: narrow-ridged finless porpoise ( Neophocaena asiaeorientalis sunameri ) oceanic dolphins (Family Delphinidae). When selecting polymerase chain reaction...
The magnetic properties and magnetoresistance of thin-film circular elliptical rings made from Co, NiFe, NiFe∕FeMn, Co∕Cu∕NiFe have been explored. Single-layer show stable onion vortex states metastable twisted containing a 360° wall. For NiFe rings, four-point magnetotransport results can be explained quantitatively by anisotropic magnetoresistance. NiFe∕FeMn exchange-biased offset hysteresis loops, the easy axis is determined combination ring ellipticity exchange coupling. In multilayer...
We report a robust quadratic relation between the inverse substrate concentration and second moment, <t2>, of catalytic turnover time distribution for enzyme reactions. The results hold irrespective mechanism dynamics reaction suggest novel single molecule experimental analysis that provides information about processes enzyme−substrate complex ergodicity system, which is beyond reach conventional mean time, <t>. It turns out <t2> − 2<t>2 linear in an ergodic homogeneous system given...