A5057040690- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- CRISPR and Genetic Engineering
- Zebrafish Biomedical Research Applications
- Receptor Mechanisms and Signaling
- Cancer, Lipids, and Metabolism
- Visual perception and processing mechanisms
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Cardiovascular Disease and Adiposity
- Genetic Syndromes and Imprinting
- Carcinogens and Genotoxicity Assessment
- Cerebrovascular and Carotid Artery Diseases
- Exercise and Physiological Responses
- Calcium signaling and nucleotide metabolism
- Blood disorders and treatments
- Color Science and Applications
- Ophthalmology and Visual Impairment Studies
- Angiogenesis and VEGF in Cancer
- Peroxisome Proliferator-Activated Receptors
- Gene Regulatory Network Analysis
- Retinal Development and Disorders
- Erythrocyte Function and Pathophysiology
- Congenital heart defects research
- Radiopharmaceutical Chemistry and Applications
Rockefeller University
2018-2021
Tri-Institutional PhD Program in Chemical Biology
2020-2021
Cornell University
2020-2021
University of California, Berkeley
2017-2021
Weill Cornell Medicine
2020
Harvard University
2014
Massachusetts General Hospital
2014
Center for Neuroscience and Regenerative Medicine
2014
McGill University
2011-2013
Lovelace Respiratory Research Institute
2010
Abstract Epigenetic aging clocks are computational models that predict age using DNA methylation information. Initially, first-generation were developed to make predictions CpGs change with age. Over time, next-generation created relate both and health. Since existing constructed in blood, we sought develop a clock optimized for prediction cheek swabs, which non-invasive easy collect. To do this, collected MethylationEPIC data as well lifestyle health information from 8045 diverse adults....
Midbrain dopamine neurons project to numerous targets throughout the brain modulate various behaviors and states. Within this small population of exists significant heterogeneity based on physiology, circuitry, disease susceptibility. Recent studies have shown that can be subdivided gene expression; however, extent which genetic markers represent functionally relevant dopaminergic subpopulations has not been fully explored. Here we performed single-cell RNA-sequencing mouse validated showing...
Significance Fast changes in membrane potential drive the unique physiology of neurons. Despite critical importance coordinated neuronal firing, observing activity a noninvasive, highly parallel manner remains an outstanding challenge, due part to lack tools that can report on fast with sufficient speed, sensitivity, and brightness. We optical voltage reporter based fluorescent rhodol shows improved photostability brightness under both one- two-photon illumination. use indicator...
NAD + , a pivotal coenzyme central to metabolism, exhibits characteristic decline with age. In mice, levels can be elevated via treatment apigenin, natural flavonoid that inhibits the -consuming glycoprotein CD38. animal models, apigenin positively impacts both sleep and longevity. For example, improves learning memory in older reduces tumor proliferation mouse xenograft model of triple-negative breast cancer, induces sedative effects mice rats. Moreover, elongates survival fly models...
The zinc finger transcriptional repressor Gfi-1b is essential for erythroid and megakaryocytic development in the embryo. Its roles maintenance of bone marrow erythropoiesis thrombopoiesis have not been defined. We investigated Gfi-1b’s adult functions using a loxP-flanked allele combination with novel doxycycline-inducible Cre transgene that efficiently mediates recombination marrow. reveal strict, lineage-intrinsic requirements continuous expression at two distinct critical stages...
Remodeling of the vessel wall and formation vascular networks are dynamic processes that occur during mammalian embryonic development in adulthood. Plaque excessive neointima hallmarks atherosclerosis injury. As our understanding these complex evolves, there is a need to develop new imaging techniques study underlying mechanisms.We used tissue clearing light-sheet microscopy for 3-dimensional (3D) profiling response carotid artery ligation induction mouse models.Adipo-Clear immunolabeling...
Dopaminergic projections exert widespread influence over multiple brain regions and modulate various behaviors including movement, reward learning, motivation. It is increasingly appreciated that dopamine neurons are heterogeneous in their gene expression, circuitry, physiology, function. Current approaches to target largely based on single drivers, which either label all or mark a subset but concurrently non-dopaminergic neurons. Here, we establish mouse line with Flpo recombinase expressed...
While earlier first-generation epigenetic aging clocks were trained to estimate chronological age as accurately possible, more recent next-generation incorporate DNA methylation information pertinent health, lifestyle, and/or outcomes. Recently, we produced a non-invasive clock using Infinium MethylationEPIC data from than 8,000 diverse adult buccal samples. this correlated with various and disease factors, did not assess its ability capture mortality. To address gap, applied CheekAge the...
Cumene is a high-production volume chemical that has been shown to be central nervous system depressant and implicated as long-term exposure carcinogen in experimental animals. The absorption, distribution, metabolism, excretion of [<sup>14</sup>C]cumene (isopropylbenzene) was studied male rats mice both sexes after oral or intravenous administration. In species sexes, urine accounted for the majority (typically ≥70%) by Enterohepatic circulation cumene and/or its metabolites indicated...
Abstract White fat stores excess energy, while brown and beige dissipate energy as heat 1 . These thermogenic adipose tissues markedly improve glucose lipid homeostasis in mouse models, though the extent to which tissue (BAT) influences metabolic cardiovascular disease humans is unclear 2, 3, 4 Here, we categorized 139,224 18 F-FDG PET/CT scans from 53,475 patients by presence or absence of BAT used propensity score matching assemble a study cohort. Individuals with showed lower prevalences...
Obesity is a major risk factor for adverse outcomes in breast cancer; however, the underlying molecular mechanisms have not been elucidated. To investigate role of crosstalk between mammary adipocytes and neoplastic cells tumor microenvironment, we performed transcriptomic analysis cancer adjacent adipose tissue murine model obesity-accelerated identified glycine amidinotransferase (Gatm) Acsbg1 as required obesity-driven progression. Gatm rate-limiting enzyme creatine biosynthesis, deletion...
OBJECTIVES/SPECIFIC AIMS: Brown adipose tissue (BAT) increases energy expenditure by dissipating chemical as heat. The combustion of glucose and lipids produces beneficial metabolic effects renders BAT an attractive target to battle obesity associated diseases. majority adults do not display active on positron emission tomography (PET) without prior cold exposure. Interestingly, a fraction individuals with positive PET scans exhibits excessive (eBAT) activity, indicating possible underlying...
Abstract Dopamine neurons project to diverse regions throughout the brain modulate various processes and behaviors. It is increasingly appreciated that dopamine are heterogeneous in their gene expression, circuitry, physiology, function. Current approaches target largely based on single drivers, which either label all neurons, or mark a sub-set but concurrently non-dopaminergic neurons. Here we establish novel mouse line Flp recombinase knocked-in endogenous Slc6a3 (dopamine active...