A5010717401- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Hemispheric Asymmetry in Neuroscience
- Vestibular and auditory disorders
- Primate Behavior and Ecology
University of California, Berkeley
2017-2021
Johns Hopkins University
2019
Significance Fast changes in membrane potential drive the unique physiology of neurons. Despite critical importance coordinated neuronal firing, observing activity a noninvasive, highly parallel manner remains an outstanding challenge, due part to lack tools that can report on fast with sufficient speed, sensitivity, and brightness. We optical voltage reporter based fluorescent rhodol shows improved photostability brightness under both one- two-photon illumination. use indicator...
The common marmoset ( Callithrix jacchus) is a promising new model for study of neurophysiological basis behavior in primates. Like other primates, it relies on saccadic eye movements to monitor and explore its environment. Previous reports have demonstrated some success training marmosets produce goal-directed actions the laboratory. However, number trials per session has been relatively small, thus limiting utility as behavioral studies. In this article, we report results series protocols...
The ability to optically record dynamics of neuronal membrane potential promises revolutionize our understanding neurobiology. In this study, we show that the far-red voltage sensitive fluorophore, Berkeley Red Sensor Transmembrane potential-1, or BeRST 1, can be used monitor changes across dozens neurons at a sampling rate 500 Hz. Notably, imaging with 1 implemented affordable, commercially available illumination sources, optics, and detectors. is well-tolerated in cultures rat hippocampal...
Voltage imaging with fluorescent dyes affords the opportunity to map neuronal activity in both time and space. One limitation is inability image complete networks: some fraction of cells remains outside observation window. Here, we combine voltage imaging, post hoc immunocytochemistry, patterned microisland hippocampal culture provide ensembles. The microislands completely fill field view our high-speed (500 Hz) camera, enabling reconstruction spiking patterns every single neuron network....
Abstract The ability to optically record dynamics of neuronal membrane potential promises revolutionize our understanding neurobiology. In this study, we show that the far-red voltage sensitive fluorophore, Berkeley Red Sensor Transmembrane −1, or BeRST 1, can be used monitor changes across dozens neurons at a sampling rate 500 Hz. Notably, imaging with 1 implemented affordable, commercially available illumination sources, optics, and detectors. is well-tolerated in cultures rat hippocampal...
Abstract Voltage imaging with fluorescent dyes affords the opportunity to map neuronal activity in both time and space. One limitation is inability image complete networks: some fraction of cells remains outside observation window. Here, we combine voltage imaging, post hoc immunocytochemistry, patterned microisland hippocampal culture provide networks. The microislands completely fill field view our high-speed (500 Hz) camera, enabling reconstruction spiking patterns every single neuron...
Abstract The common marmoset ( Callithrix Jacchus ) is a promising new model for study of neurophysiological basis behavior in primates. Like other primates, it relies on saccadic eye movements to monitor and explore its environment. Previous reports have demonstrated some success training marmosets produce goal-directed actions the laboratory. However, number trials per session has been relatively small, thus limiting utility as behavioral studies. Here, we report results series protocols...