A5072920557- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- Hemoglobin structure and function
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Glioma Diagnosis and Treatment
- Hemoglobinopathies and Related Disorders
- Parkinson's Disease Mechanisms and Treatments
- Neural dynamics and brain function
- Neonatal Health and Biochemistry
- Nerve injury and regeneration
- Neurological disorders and treatments
- Nicotinic Acetylcholine Receptors Study
- Memory and Neural Mechanisms
- Cellular transport and secretion
- Heme Oxygenase-1 and Carbon Monoxide
- Meningioma and schwannoma management
- Parasites and Host Interactions
- Endoplasmic Reticulum Stress and Disease
- Stress Responses and Cortisol
- Lipid Membrane Structure and Behavior
- Neuroscience and Neural Engineering
- Mitochondrial Function and Pathology
Yale University
2015-2024
New Haven Public Schools
2023
Hokkaido University
2003-2006
University of California, San Francisco
2001-2006
Hokuto Hospital
2006
The University of Tokyo
1998-2006
Czech Academy of Sciences, Institute of Physiology
2005
Dokkyo University
2002
National Institute for Basic Biology
2002
Okayama University
1991-2001
Functional expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors in cerebellar granule cells requires stargazin, a member large family four-pass transmembrane proteins. Here, we define AMPA receptor regulatory proteins (TARPs), which comprise γ-3, γ-4, and γ-8, but not related proteins, that mediate surface receptors. TARPs exhibit discrete complementary patterns both neurons glia the developing mature central nervous system. In brain regions express multiple...
Activity-dependent plasticity in the brain arises part from changes number of synaptic AMPA receptors. Synaptic trafficking receptors is controlled by stargazin and homologous transmembrane receptor regulatory proteins (TARPs). We found that TARPs were stable at plasma membrane, whereas internalized a glutamate-regulated manner. Interaction with involved both extra- intracellular determinants TARPs. Upon binding to glutamate, detached This did not require ion flux or second messengers....
A fundamental question in sensory neuroscience is how parallel processing implemented at the level of molecular and circuit mechanisms. In retina, it has been proposed that distinct OFF cone bipolar cell types generate fast/transient slow/sustained pathways by differential expression AMPA- kainate-type glutamate receptors, respectively. However, functional significance these receptors intact during light stimulation remains unclear. Here, we measured release from mouse cells two-photon...
β-Amyloid peptide (Aβ) is a principal component of parenchymal amyloid deposits in Alzheimer's disease. Aβ derived from precursor protein (APP) by proteolytic cleavage. APP subject to N- and O-glycosylation potential tyrosine sulfation, following synthesis, then thought be cleaved an intracellular secretory pathway after or during these post-translational modifications. Studies utilizing agents that affect series steps the have identified possible sites cleavage generation within pathway. In...
A novel protein, human X11-like (human X11L), contains a phosphotyrosine interaction (PI) domain and two PDZ domains displays 55.2% amino acid homology with the X11 X11). The PI of X11L interacts sequence containing NPXY motif found in cytoplasmic Alzheimer's amyloid precursor protein. construct lacking carboxyl-terminal domain, which comprises (N + PI), enhances binding to APP, whereas another an amino-terminal relative (PI C) suppresses APP. Overexpression full-length cells that express...
Previously we found that X11-like protein (X11L) associates with amyloid beta-protein precursor (APP). X11L stabilizes APP metabolism and suppresses the secretion of (Abeta) are pathogenic agents Alzheimer's disease (AD). Here Alcadein (Alc), a novel membrane family contains cadherin motifs originally reported as calsyntenins, also interacted X11L. Alc was abundant in brain occurred same areas could simultaneously associate Alc, resulting formation tripartite complex brain. The stabilized...
We determined the frequency of dropped head syndrome in Parkinson's disease (PD) Japan and evaluated its clinical correlates. A total 252 consecutive patients with PD who visited our hospital were studied. Dropped was found 15 (6.0%) (3 men, 12 women; mean age at onset PD, 62.8 +/- 11.5 years). The interval before emergence after 5.4 4.3 years (-0.5 to Hoehn-Yahr score on stage 3.2 0.7; off 3.5 0.8. Of those patients, 8 had major symptoms rigidity akinesia. In 2 administration a dopamine...
The oxygen affinity of mouse hemoglobin is greatly lowered by 2,3-diphosphoglycerate (2,3-DPG). higher the pH, smaller size effect. pH dependence this shift in log p50 resembles a titration curve with an apparent pK 7.76. This value close to that reported for α-NH2 groups deoxyhemoglobin. addition 1 2 moles 2,3-DPG per mole decreases n Hill's equation. Further causes return normal values. However, estimation overall free energy interaction shows does not affect interaction. changes are...
Loss-of-function mutations in the depalmitoylating enzyme palmitoyl protein thioesterase 1 (PPT1) cause neuronal ceroid lipofuscinosis (NCL), a devastating neurodegenerative disease. The substrates of PPT1 are largely undescribed, posing limitation on molecular dissection disease mechanisms and therapeutic development. Here, we provide resource identifying >100 novel substrates. We utilized Acyl Resin-Assisted Capture (Acyl RAC) mass spectrometry to identify proteins with increased vivo...