A5000360108- Eosinophilic Disorders and Syndromes
- Chronic Myeloid Leukemia Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cytokine Signaling Pathways and Interactions
- Soft tissue tumor case studies
- Neuroblastoma Research and Treatments
- Diet and metabolism studies
- Sarcoma Diagnosis and Treatment
- FOXO transcription factor regulation
- Liver Disease Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Diet, Metabolism, and Disease
- Immune Cell Function and Interaction
- Ubiquitin and proteasome pathways
- Neuroendocrine Tumor Research Advances
- Cholesterol and Lipid Metabolism
- Gut microbiota and health
- Lung Cancer Research Studies
- Mast cells and histamine
- Protein Tyrosine Phosphatases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Lymphoma Diagnosis and Treatment
- Cancer Mechanisms and Therapy
- Peroxisome Proliferator-Activated Receptors
- Drug Transport and Resistance Mechanisms
de Duve Institute
2015-2024
UCLouvain
2013-2022
Cliniques Universitaires Saint-Luc
2010-2013
PharmacoGenetics (China)
2011
Ludwig Cancer Research
2003-2006
Uppsala University
2006
Ludwig Cancer Research
1996-2003
The gut microbiota regulates key hepatic functions, notably through the production of bacterial metabolites that are transported via portal circulation. We evaluated effects produced by from aromatic amino acids (phenylacetate, benzoate, p-cresol, and indole) on liver inflammation induced endotoxin. Precision-cut slices prepared control mice, Kupffer cell (KC)-depleted obese mice ( ob/ ob) were treated with or without LPS metabolites. observed beneficial indole dose-dependently reduced...
Objective To investigate the beneficial role of prebiotics on endothelial dysfunction, an early key marker cardiovascular diseases, in original mouse model linking steatosis and dysfunction. Design We examined contribution gut microbiota to vascular dysfunction observed apolipoprotein E knockout (Apoe −/− ) mice fed n-3 polyunsaturated fatty acid (PUFA)-depleted diet for 12 weeks with or without inulin-type fructans (ITFs) supplementation last 15 days. Mesenteric carotid arteries were...
FOXO (Forkhead box O) transcription factors induce cell growth arrest and apoptosis, which can be prevented by phosphorylation AKT in response to such as platelet-derived (PDGF) insulin-like factor I (IGF-I). In addition this well characterized post-translational modification, we showed that FOXO1, FOXO3, FOXO4 were also regulated at the transcriptional level. PDGF, fibroblast (FGF), IGF-I repressed expression of genes human fibroblasts. This process was sensitive phosphatidylinositol...
Deciphering transcription factor networks from microarray data remains difficult. This study presents a simple method to infer the regulation of factors based on well-characterized target genes. We generated catalog containing associated with 2720 genes and 6401 experimentally validated regulations. When it was available, distinction between transcriptional activation inhibition included for each regulation. Next, we built tool (www.tfacts.org) that compares submitted gene lists in detect...
AbstractInterleukin-9 (IL-9), a T-cell-derived cytokine, interacts with specific receptor associated the IL-2 γ chain. In this report, we analyze functional domains of human IL-9 transfected into mouse lymphoid cell lines. Three different functions were examined: growth stimulation in factor-dependent pro-B Ba/F3 cells, protection against dexamethasone-induced apoptosis, and Ly-6A2 induction BW5147 lymphoma cells. The results indicated that single tyrosine, at position 116 cytoplasmic...
Abstract Obesity is a pandemic disease associated with many metabolic alterations and involves several organs systems. The endocannabinoid system (ECS) appears to be key regulator of energy homeostasis metabolism. Here we show that specific deletion the ECS synthesizing enzyme, NAPE-PLD, in adipocytes induces obesity, glucose intolerance, adipose tissue inflammation altered lipid We report Napepld -deleted mice present an browning programme are less responsive cold-induced browning,...
To examine the role of hepatocyte myeloid differentiation primary-response gene 88 (MyD88) on glucose and lipid metabolism.To study impact innate immune system at level metabolism, we generated mice harbouring hepatocyte-specific deletion MyD88. We investigated metabolism by feeding with a normal control diet or high-fat for 8 weeks. evaluated body weight, fat mass gain (using time-domain nuclear magnetic resonance), energy homeostasis metabolic chambers). performed microarrays quantitative...
Well-differentiated small intestinal neuroendocrine tumors are rare malignancies. They arise from enterochromaffin cells and very little is known about differential microRNA (miRNA) expression. The aim of this study was to identify the miRNA profile well-differentiated tumors, which may have a critical role in tumor development, progression potentially develop miRNAs as novel clinical biomarkers. Specimens two test groups, 24 specimens at different stages malignancy, included study. Total...
Rationale: There is evidence that CD4+ effector T lymphocytes (T eff) participate in the development of lung fibrosis, but role their regulatory T-cell reg) counterparts remains to be determined.Objectives: To elucidate contribution reg cells a mouse model fibrosis induced by silica (SiO2) particles.Methods: Lung and eff purified from SiO2–treated Foxp3-GFP transgenic mice were cocultured with naive fibroblasts or transferred lungs healthy mice. DEREG mice, which express diphtheria toxin...
Infantile myofibromatosis is one of the most prevalent soft tissue tumors infancy and childhood. Multifocal nodules with visceral lesions are associated a poor prognosis. A few familial cases have been linked to mutations in various genes including PDGFRB. In this study, we sequenced PDGFRB, which encodes receptor tyrosine kinase, 16 or solitary myofibroma. Mutations coding sequence PDGFRB were identified 6 out 8 patients sporadic multicentric form disease 1 isolated Two had same mutation...
The majority of polycythemia vera (PV) patients harbor a unique somatic mutation (V617F) in the pseudokinase domain JAK2, which leads to constitutive signaling. Here we show that homologous mutations JAK1 (V658F) and Tyk2 (V678F) lead activation these kinases. Their expression induces autonomous growth cytokine-dependent cells STAT5, STAT3, mitogen-activated protein kinase, Akt signaling Ba/F3 cells. mutant JAKs exhibit also when expressed fibrosarcoma deficient JAK proteins. Expression JAK2...
Perturbation of lipid metabolism favours progression Alzheimer disease, in which processing Amyloid Precursor Protein (APP) has important implications. APP cleavage is tightly regulated by cholesterol and fragments regulate homeostasis. Here, we investigated whether up or down regulation full-length expression affected neuronal metabolism. Expression decreased HMG-CoA reductase (HMGCR)-mediated biosynthesis SREBP mRNA levels, while its had opposite effects. SREBP1 co-immunoprecipitated...
Systemic sclerosis (SSc) is a severe connective tissue disease of unknown etiology, characterized by fibrosis the skin and multiple internal organs. Recent findings suggested that driven stimulatory autoantibodies to platelet-derived growth factor receptor (PDGFR), which stimulate production reactive oxygen species (ROS) collagen fibroblasts. These results opened novel avenues research into diagnosis treatment SSc. The present study was undertaken confirm presence anti-PDGFR antibodies in...
Recent data suggest that gut microbiota contributes to the regulation of host lipid metabolism. We report how fermentable dietary fructo-oligosaccharides (FOS) control hepatic steatosis induced by n-3 PUFA depletion, which leads alterations similar those observed in non-alcoholic fatty liver disease patients.C57Bl/6J mice fed an PUFA-depleted diet for 3 months were supplemented with FOS during last 10 days treatment. FOS-treated exhibited higher caecal Bifidobacterium spp. and lower...
Patients with non-alcoholic fatty liver disease are characterised by a decreased n-3/n-6 polyunsaturated acid (PUFA) ratio in hepatic phospholipids. The metabolic consequences of n-3 PUFA depletion the poorly understood. We have reproduced drastic drop among phospholipids feeding C57Bl/6J mice for 3 months an depleted diet (DEF) versus control (CT), which only differed content. DEF exhibited insulin resistance (assessed euglycemic-hyperinsulinemic clamp) and steatosis that was associated...
Myofibroma is the most frequent fibrous tumor in children. Multicentric myofibroma (referred to as infantile myofibromatosis) a life-threatening disease.To determine frequency, spectrum, and clinical implications of mutations PDGFRB receptor tyrosine kinase found sporadic myofibromatosis.In this retrospective study 69 patients with or myofibromatosis, 85 samples were obtained analyzed by targeted deep sequencing PDGFRB. Mutations confirmed an alternative method experimentally characterized...
The gut microbiota makes critical contributions to host homeostasis, and its role in the treatment of acute myeloid leukaemia (AML) has attracted attention. We investigated whether microbiome is affected by AML, such changes are associated with cachectic hallmarks. Biological samples clinical data were collected from 30 antibiotic-free AML patients at diagnosis matched volunteers (1:1) a multicenter cross-sectional prospective study. composition functional potential faecal analyzed using...