A5005755141- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- FOXO transcription factor regulation
- Diet, Metabolism, and Disease
- Neuroendocrine Tumor Research Advances
- Immunotherapy and Immune Responses
- Eosinophilic Disorders and Syndromes
- Bioinformatics and Genomic Networks
- Monoclonal and Polyclonal Antibodies Research
- Gene expression and cancer classification
- Lung Cancer Research Studies
- Gut microbiota and health
- Cholesterol and Lipid Metabolism
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- T-cell and B-cell Immunology
- Neuroblastoma Research and Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Genomics and Chromatin Dynamics
- Machine Learning in Bioinformatics
- Peptidase Inhibition and Analysis
- MicroRNA in disease regulation
- Pluripotent Stem Cells Research
- Gastrointestinal Tumor Research and Treatment
- RNA modifications and cancer
- Immune Response and Inflammation
GlaxoSmithKline (Belgium)
2020-2024
de Duve Institute
2008-2021
UCLouvain
2008-2020
Objective To investigate the beneficial role of prebiotics on endothelial dysfunction, an early key marker cardiovascular diseases, in original mouse model linking steatosis and dysfunction. Design We examined contribution gut microbiota to vascular dysfunction observed apolipoprotein E knockout (Apoe −/− ) mice fed n-3 polyunsaturated fatty acid (PUFA)-depleted diet for 12 weeks with or without inulin-type fructans (ITFs) supplementation last 15 days. Mesenteric carotid arteries were...
FOXO (Forkhead box O) transcription factors induce cell growth arrest and apoptosis, which can be prevented by phosphorylation AKT in response to such as platelet-derived (PDGF) insulin-like factor I (IGF-I). In addition this well characterized post-translational modification, we showed that FOXO1, FOXO3, FOXO4 were also regulated at the transcriptional level. PDGF, fibroblast (FGF), IGF-I repressed expression of genes human fibroblasts. This process was sensitive phosphatidylinositol...
Deciphering transcription factor networks from microarray data remains difficult. This study presents a simple method to infer the regulation of factors based on well-characterized target genes. We generated catalog containing associated with 2720 genes and 6401 experimentally validated regulations. When it was available, distinction between transcriptional activation inhibition included for each regulation. Next, we built tool (www.tfacts.org) that compares submitted gene lists in detect...
Abstract Obesity is a pandemic disease associated with many metabolic alterations and involves several organs systems. The endocannabinoid system (ECS) appears to be key regulator of energy homeostasis metabolism. Here we show that specific deletion the ECS synthesizing enzyme, NAPE-PLD, in adipocytes induces obesity, glucose intolerance, adipose tissue inflammation altered lipid We report Napepld -deleted mice present an browning programme are less responsive cold-induced browning,...
To examine the role of hepatocyte myeloid differentiation primary-response gene 88 (MyD88) on glucose and lipid metabolism.To study impact innate immune system at level metabolism, we generated mice harbouring hepatocyte-specific deletion MyD88. We investigated metabolism by feeding with a normal control diet or high-fat for 8 weeks. evaluated body weight, fat mass gain (using time-domain nuclear magnetic resonance), energy homeostasis metabolic chambers). performed microarrays quantitative...
Well-differentiated small intestinal neuroendocrine tumors are rare malignancies. They arise from enterochromaffin cells and very little is known about differential microRNA (miRNA) expression. The aim of this study was to identify the miRNA profile well-differentiated tumors, which may have a critical role in tumor development, progression potentially develop miRNAs as novel clinical biomarkers. Specimens two test groups, 24 specimens at different stages malignancy, included study. Total...
Recent data suggest that gut microbiota contributes to the regulation of host lipid metabolism. We report how fermentable dietary fructo-oligosaccharides (FOS) control hepatic steatosis induced by n-3 PUFA depletion, which leads alterations similar those observed in non-alcoholic fatty liver disease patients.C57Bl/6J mice fed an PUFA-depleted diet for 3 months were supplemented with FOS during last 10 days treatment. FOS-treated exhibited higher caecal Bifidobacterium spp. and lower...
Patients with non-alcoholic fatty liver disease are characterised by a decreased n-3/n-6 polyunsaturated acid (PUFA) ratio in hepatic phospholipids. The metabolic consequences of n-3 PUFA depletion the poorly understood. We have reproduced drastic drop among phospholipids feeding C57Bl/6J mice for 3 months an depleted diet (DEF) versus control (CT), which only differed content. DEF exhibited insulin resistance (assessed euglycemic-hyperinsulinemic clamp) and steatosis that was associated...
Hepatitis B vaccines formulated with different adjuvants induce a consistent core inflammatory signature linked to vaccine immunogenicity.
Abstract Platelet‐derived growth factors ( PDGF ) bind to two related receptor tyrosine kinases, which are encoded by the PDGFRA and PDGFRB genes. Recently, heterozygous mutations have been described in patients diagnosed with idiopathic basal ganglia calcification IBGC or Fahr disease), a rare inherited neurological disorder. The goal of present study was determine whether these had positive negative impact on activity. We first showed that E1071V mutant behaved like wild‐type may represent...
Abstract The mechanisms by which antibodies confer protection vary across vaccines, ranging from simple neutralization to functions requiring innate immune recruitment via Fc-dependent mechanisms. role of adjuvants in shaping the maturation antibody-effector remains under investigated. Using systems serology, we compared licensed vaccines (AS01 B /AS01 E /AS03/AS04/Alum) combined with a model antigen. Antigen-naive adults received two adjuvanted immunizations followed late revaccination...
The adjuvant AS01 plays a key role in the immunogenicity of several approved human vaccines with demonstrated high efficacy. Its effect relies on activation innate immune system. However, specific effects AS01-adjuvanted cell function and epigenetic remodeling, as described for Bacille Calmette-Guérin (BCG) influenza vaccines, are still unknown. We assessed long-term functional changes circulating monocytes dendritic cells induced by model vaccine containing hepatitis B surface antigen...
Background Small intestine neuroendocrine tumors (SI-NETs) belong to a rare group of cancers. Most patients have developed metastatic disease at the time diagnosis, for which there is currently no cure. The delay in diagnosis major issue clinical management and new markers are urgently needed. We previously identified paraneoplastic antigen Ma2 (PNMA2) as novel SI-NET tissue biomarker. Therefore, we evaluated whether autoantibodies detection blood stream useful recurrence SI-NETs....
A universal cancer biomarker candidate for diagnosis is supposed to distinguish, within a broad range of tumors, between healthy and diseased patients. Recently published studies have explored the usefulness some biomarkers in human tumors. In this study, we present an integrative approach search potential common biomarkers. Using TFactS web-tool with catalogue experimentally established gene regulations, could predict transcription factors (TFs) regulated 305 different cell lines covering...
Background ETV6-PDGFRB (also called TEL-PDGFRB) and FIP1L1-PDGFRA are receptor-tyrosine kinase fusion genes that cause chronic myeloid malignancies associated with hypereosinophilia. The aim of this work was to gain insight into the mechanisms whereby affect human hematopoietic cells in particular eosinophil lineage.Design Methods We introduced CD34+ progenitor stem isolated from umbilical cord blood.Results Cells transduced these oncogenes formed colonies even absence cytokines. Both also...
Cytarabine and daunorubicin are old drugs commonly used in the treatment of acute myeloid leukaemia (AML). Refractory or relapsed disease because chemotherapy resistance is a major issue. microRNAs (miRNAs) were incriminated resistance. This study aimed to identify miRNAs involved chemoresistance AML patients define their target genes. We focused on cytogenetically normal with wild-type NPM1 without FLT3-ITD as this subset intermediate-risk cytogenetics not well established. analysed...
Sterol-regulatory element binding proteins (SREBPs) control the expression of genes involved in fatty acid and cholesterol biosynthesis. Using microarrays, we observed that mature SREBP-1 also induced unrelated to lipid metabolism, such as heme oxygenase 1 (HMOX1), plasma glutathione peroxidase, phosphatidylinositol-3 kinase regulatory subunit p55 gamma, synaptic vesicle glycoprotein 2A, COTE1. The these was repressed upon addition sterols, which block endogenous SREBP cleavage, by statin...
Octreotide is a widely used synthetic somatostatin analogue that significantly improves the management of neuroendocrine tumours (NETs). acts through receptors (SSTRs). However, molecular mechanisms leading to successful disease control or symptom management, especially when SSTRs levels are low, largely unknown. We provide novel insights into how octreotide controls NET cells. CNDT2.5 cells were treated from 1 day up 16 months with and then profiled using Affymetrix microarray analysis....
Activating transcription factor (ATF)3 regulates the expression of inflammation-related genes in several tissues under pathological contexts. In skeletal muscle, atf3 increases after exercise, but its target remain unknown. We aimed to identify those and determine influence ATF3 on muscle adaptation training. Skeletal muscles ATF3-knockout (ATF3-KO) control mice were analyzed at rest, resting muscles, there was no difference between genotypes enzymatic activities or fiber type. After a...
Anthracyclines remain a cornerstone of induction chemotherapy for acute myeloid leukemia (AML). Refractory or relapsed disease due to resistance is major obstacle in AML management. MicroRNAs (miRNAs) have been observed be involved chemoresistance. We previously that miR-15a-5p was overexpressed subgroup chemoresistant cytogenetically normal patients compared with chemosensitive treated daunorubicin and cytarabine. MiR-15a-5p overexpression cells reduced apoptosis induced by both drugs...
Growth factors inactivate the FOXO (forkhead box O) transcription through PI3K (phosphoinositide 3-kinase) and PKB (protein kinase B). By comparing microarray data from multiple model systems, we identified HBP1 (high-mobility group-box protein 1) as a novel downstream target of this pathway. mRNA was down-regulated by PDGF (platelet-derived growth factor), FGF (fibroblast PKB, whereas it up-regulated factors. This observation confirmed in human murine fibroblasts well cell lines derived...
Transforming growth factor-β (TGFβ) is a key mediator of fibrogenesis. TGFβ overexpressed and activated in fibrotic diseases, regulates fibroblast differentiation into myofibroblasts induces extracellular matrix deposition. Platelet-derived factor (PDGF) also regulator Some studies showed link between PDGF certain diseases. receptor alpha expression scleroderma fibroblasts. PDGF-C -D are the most recently discovered ligands play role fibrosis. In this study, we report first PDGF-D -C...