A5069411349- Microfluidic and Bio-sensing Technologies
- Spaceflight effects on biology
- Magnetic and Electromagnetic Effects
- Bone health and osteoporosis research
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Planarian Biology and Electrostimulation
- Muscle Physiology and Disorders
- Digital Holography and Microscopy
- Connexins and lens biology
- Microfluidic and Capillary Electrophoresis Applications
- Adipose Tissue and Metabolism
- Bone fractures and treatments
- Bone Tissue Engineering Materials
- Advanced ceramic materials synthesis
- Bone Metabolism and Diseases
- Planetary Science and Exploration
- Polymer Foaming and Composites
- RNA Research and Splicing
- Effects of Vibration on Health
- Tendon Structure and Treatment
- Electromagnetic Fields and Biological Effects
- Primate Behavior and Ecology
- Pleistocene-Era Hominins and Archaeology
- Nuclear Structure and Function
Izmir Institute of Technology
2016-2025
Stony Brook University
2004-2014
State University of New York
2005-2008
Abstract Magnetic levitation though negative magnetophoresis is a novel technology to simulate weightlessness and has recently found applications in material biological sciences. Yet little known about the ability of magnetic system facilitate biofabrication situ three dimensional (3D) cellular structures. Here, we optimized protocol appropriate for long term levitated cell culture developed an 3D assembly model with controlled cluster size pattern under simulated weightlessness. The...
Mechanical disuse will bias bone marrow stromal cells towards adipogenesis, ultimately compromising the regenerative capacity of stem cell pool and impeding rapid full recovery morphology. Here, it was tested whether brief daily exposure to high-frequency, low-magnitude vibrations can preserve environment during enhance initiation tissue upon reambulation. Male C57BL/6J mice were subjected hindlimb unloading (HU, n = 24), HU interrupted by weight-bearing for 15 min/d (HU+SHAM, low-level...
ABSTRACT Inference of feeding adaptation in extinct species is challenging, and reconstructions the paleobiology our ancestors have utilized an array analytical approaches. Comparative anatomy finite element analysis assist bracketing range capabilities taxa, while microwear isotopic analyses give glimpses individual behavior past. These myriad approaches limitations, but each contributes incrementally toward recognition hominin fossil record. Microwear stable isotope together suggest that...
Adipocyte hypertrophy and hyperplasia are important parameters in describing abnormalities adipogenesis that concomitant to diseases such as obesity, diabetes, anorexia nervosa osteoporosis. Therefore, technical developments the detection of adipocytes become an driving factor research. Current techniques optical microscopy flow cytometry available examination adipocytes, cell- molecular-based research adipogenesis. Even though common straightforward, they restricted terms manipulation...
Application of mechanical vibrations is anabolic to bone tissue, not only by guiding mature cells increased formation, but also increasing the osteogenic commitment progenitor cells. However, sensitivity and adaptive response marrow stem this loading regimen has yet been identified. In study, we subjected mouse cell line D1-ORL-UVA daily (0.15 g, 90 Hz, 15 min/day) for 7 days, both during quiescence commitment, identify corresponding ultrastructural adaptations on cellular molecular levels....
Bone marrow mesenchymal stem cells that are committed to adipogenesis were exposed daily high-frequency low-intensity mechanical vibrations understand molecular, morphological and ultrastructural adaptations signals during adipogenesis. D1-ORL-UVA mouse bone cultured with either growth or adipogenic medium for 1 week. Low-intensity vibration (15 min/day, 90 Hz, 0.1 g) applied one group of cells, while the other served as a sham control. Cellular viability, lipid accumulation, ultrastructure...
In clinical practice, a variety of diagnostic applications require the identification target cells. Density has been used as physical marker to distinguish cell populations since metabolic activities could alter densities. Magnetic levitation offers great promise for separating cells at single level within heterogeneous with respect Traditional magnetic platforms need bulky and precise optical microscopes visualize levitated Moreover, evaluation process densities is cumbersome, which also...
Diamagnetic levitation is an emerging technology for remote manipulation of cells in cell and tissue level applications. Low-cost magnetic configurations using permanent magnets are commonly composed a culture chamber physically sandwiched between two block that limit working volume applicability. This work describes single ring magnet-based system to eliminate physical limitations biofabrication. Developed configuration utilizes sample construct size long-term maintenance. Furthermore, our...
Closed‐cell foams were fabricated by incorporating two different grades of hollow alumino‐silicate spheres (cenospheres) into a silicon carbide matrix. The matrix was formed the pyrolysis preceramic polymer, and multiple polymer infiltration (PIP) cycles employed to minimize open voids in material. physical, mechanical thermal properties characterized as functions number reinfiltration cycles. open‐ closed‐void volume fractions determined measurements bulk skeletal densities. Mechanical...
Mechanical loads can regulate cell proliferation and differentiation at various stages of development homeostasis. However, the extension this regulatory effect mechanical on cancer cells is largely unknown. Increased physical compliance one key features cells, which may hamper transmission to these within tumor microenvironment. Here we tested whether brief daily application an external low magnitude stimulus (LMMS), would impede growth MDA-MB-231 aggressive type breast in vitro for 3 wks...
The aim of this study was to develop random and aligned polyacrilonitrile (PAN)/polypyrrole (PPy) nanofibrous scaffolds by electrospinning technique for osteogenic differentiation mesenchymal stem cells. Nanofibers were fabricated successfully as straight, smooth, free from bead formation. average diameter nanofibers 268(±49) nm 225(±72) nm, respectively. Alignment process increased the tensile strength 3.9-fold, while strain decreased 78%. PAN/PPy hydrophilic with contact angle value about...
Abstract Tissue engineering research aims to repair the form and/or function of impaired tissues. studies mostly rely on scaffold‐based techniques. However, these techniques have certain challenges, such as selection proper scaffold material, including mechanical properties, sterilization, and fabrication processes. As an alternative, we propose a novel scaffold‐free adipose tissue biofabrication technique based magnetic levitation. In this study, label‐free levitation was used...
Mesenchymal stem cells (MSCs) maintain the musculoskeletal system by differentiating into multiple lineages, including osteoblasts and adipocytes. Mechanical signals, strain low-intensity vibration (LIV), are important regulators of MSC differentiation via control exerted through cell structure. Lamin A/C is a protein vital to nuclear architecture that supports chromatin organization contributes mechanical integrity nucleus. We investigated whether lamin mechanoresponsiveness functionally...