Abstract Magnetic levitation though negative magnetophoresis is a novel technology to simulate weightlessness and has recently found applications in material biological sciences. Yet little known about the ability of magnetic system facilitate biofabrication situ three dimensional (3D) cellular structures. Here, we optimized protocol appropriate for long term levitated cell culture developed an 3D assembly model with controlled cluster size pattern under simulated weightlessness. The...

Single cells have unique biophysical signatures that can rapidly change during various disease states.

Intrinsic density-based particle separation is fundamental to biomedical research and materials science. Magnetic levitation offers an accessible label-free approach; however, current platforms are limited by throughput, complex fabrication requirements, manual analysis methods. Here, we demonstrate a high-throughput magnetic levitation-based microfluidic device fabricated using commercial 3D printing, integrated with dual automated systems. The features optimized magnet configurations wide...

This design demonstrates the isolation of extracellular vesicles (EVs) from human plasma, utilizing polymer beads as assay surfaces to capture EV subpopulations and magnetic levitation sort at different outlets.

Magnetic levitation, which is a magnetic phenomenon of levitating particles suspended in paramagnetic liquid under nonuniform field, powerful tool for determining densities and properties micro- nanoparticles. The levitation height the field depends on susceptibility density difference between object surrounding liquid. Here, we developed susceptibility-based protein detection scheme low-cost miniaturized setup consisting two opposing magnets to create gradient glass capillary channel retain...

In clinical practice, a variety of diagnostic applications require the identification target cells. Density has been used as physical marker to distinguish cell populations since metabolic activities could alter densities. Magnetic levitation offers great promise for separating cells at single level within heterogeneous with respect Traditional magnetic platforms need bulky and precise optical microscopes visualize levitated Moreover, evaluation process densities is cumbersome, which also...

Peripheral lymphocyte DNA damage as measured by the method of alkaline filter elution and frequency sister chromatid exchange (SCE) in lymphocytes was investigated for a group 20 female workers shoemaking plant who were exposed to benzene toluene, primarily below German threshold limit value 5 100 p.p.m. respectively; results compared with those from control group. In significantly raised (P less than 0.05) SCE values found. The relative rate through polycarbonate filters increased 0.001)....

Abstract Self‐assembly of cells into functional bioarchitectures with microscale spatial topographies are prevalent in nature. Despite the advances to recapitulate native‐like microenvironment vitro,fabrication soft living materials a deterministic control on composition, functionality, and geometry, is still challenging. Here, versatile, paramagnetically tunable, levitational biofabrication technique within ring‐magnet system, RM‐LEV, developed assemble single or heterogeneous multicellular...

Gravity is an important biomechanical signal effecting the morphology and function of organisms. Reduction gravitational forces, as experienced during spaceflight, cause alterations in biological systems. Magnetic levitation technique one most recent ground-based technology to mimic weightlessness environment. In addition providing a platform investigate effects weightlessness, this presents novel opportunity biofabricate 3-dimensional (3D) structures scaffold-and nozzle-free fashion. study,...

Separation of target cells in a heterogeneous solution is great importance for clinical studies especially immunology and oncology. Separated can be used diagnostic applications ranging from whole blood counting to isolation circulating tumor (CTC) personalized medicine. Recent separation technologies rely on labelling identifying with variety principle such as fluorescence or magnetic tags. However, they require labor-intensive processes, long analysis time, expensive chemical reagents...

Abstract The aim of this work was to develop an effective fed‐batch feeding strategy enhance recombinant glucose isomerase (r‐GI) production by Escherichia coli BL21 (DE3) pLysS on industrially relevant feedstock without the application exogenous inducer. Following batch operation (0 < t 7 H), effects pulse and/or continuous hydrolyzed beet molasses were investigated under five different strategies. two most promising strategies with respect r‐GI activity (i) PM‐0.05, designed one feed (...

Abstract Circulating hybrid cells (CHCs) are a novel, rare cell population that harbor tumor and immune phenotypes genotypes detectible in peripheral blood. Several recent reports implicated CHCs the metastatic cascade found their enumeration to provide better prognostic value than conventionally-defined circulating (CTCs). However, methods for isolation enrichment of not well-studied or established. Here, we developed an ultrasensitive, antigen-independent platform leveraging principles...

Microparticle/cell separation is one of the most important applications in field biomedical sciences particularly for cell sorting and protein assays. There are variety different technologies introduced literature that main limitations large amount sample, expensive chemical use besides requirement a labeling procedure (i.e. fluorescent/magnetic labeling), complex machinery, high operational costs. Magnetic levitation- based offers simple, rapid precise microparticles on their densities by...

Abstract In clinical practice, a variety of diagnostic applications require the identification target cells. Density has been used as physical marker to distinguish cell populations since metabolic activities could alter densities. Magnetic levitation offers great promise for separating cells at single level within heterogeneous with respect Traditional magnetic platforms need bulky and precise optical microscopes visualize levitated Moreover, evaluation process densities is cumbersome,...

Label-free magnetic levitation is one of the most recent Earth-based in vitro t echniques that simulate microgravity. This technique offers a great opportunity to biofabricate scaffold-free 3-dimensional (3D) structures and study effects microgravity on these structures. In this study, self-assembled 3D living were fabricated paramagnetic medium by cellular health was assessed. force-assisted assembly system applied here broad applications several fields, such as space biotechnology...