A5024631265- Autophagy in Disease and Therapy
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Metabolomics and Mass Spectrometry Studies
- Endoplasmic Reticulum Stress and Disease
- Analytical Chemistry and Chromatography
- Viral Infectious Diseases and Gene Expression in Insects
- Mitochondrial Function and Pathology
- Virus-based gene therapy research
- Cellular transport and secretion
- Protein purification and stability
- Sphingolipid Metabolism and Signaling
- Erythrocyte Function and Pathophysiology
- Healthcare and Venom Research
- Fungal Biology and Applications
- GABA and Rice Research
- Genetic and Kidney Cyst Diseases
- Biochemical and Molecular Research
- Insect and Pesticide Research
- Renal Diseases and Glomerulopathies
- Microfluidic and Bio-sensing Technologies
- Neuroscience and Neuropharmacology Research
- Biochemical Acid Research Studies
- Alzheimer's disease research and treatments
Lonza (Switzerland)
2021-2025
University of Freiburg
2011-2018
Institute for Systems Analysis
2013
University of Münster
2006-2008
Pliva (Croatia)
2008
University of Mazandaran
2002
Damage to and loss of glomerular podocytes has been identified as the culprit lesion in progressive kidney diseases. Here, we combine mass spectrometry-based proteomics with mRNA sequencing, bioinformatics, hypothesis-driven studies provide a comprehensive quantitative map mammalian that identifies unanticipated signaling pathways. Comparison vivo datasets data from podocyte cell cultures showed limited value available culture models. Moreover, stable isotope labeling by amino acids...
Reversible phosphorylations play a critical role in most biological pathways. Hence, signaling studies great effort has been put into identification of maximum number phosphosites per experiment. Mass spectrometry (MS)-based phosphoproteomics approaches have proven to be an ideal analytical method for mapping phosphosites. However, because sample complexity, fractionation phosphopeptides prior MS analysis is crucial step. In the current study, we compare chromatographic strategies...
Under conditions of nutrient shortage autophagy is the primary cellular mechanism ensuring availability substrates for continuous biosynthesis. Subjecting cells to starvation or rapamycin efficiently induces by inhibiting MTOR signaling pathway triggering increased autophagic flux. To elucidate regulation early events upon induction, we applied quantitative phosphoproteomics characterizing temporal phosphorylation dynamics after and treatment. We obtained a comprehensive atlas kinetics...
Macroautophagy is regarded as a nonspecific bulk degradation process of cytoplasmic material within the lysosome. However, has mainly been studied by assays using radiolabeling. In present study we monitor protein turnover and global, unbiased approaches relying on quantitative mass spectrometry-based proteomics. induced rapamycin treatment, amino acid glucose starvation in differentially, metabolically labeled cells. Protein dynamics are linked to image-based models autophagosome turnover....
A superior approach involving nano-high-performance liquid chromatography (nano-HPLC) in on-line conjunction to electrospray ionization quadrupole time-of-flight mass spectrometry (ESI QTOF MS) and tandem MS for screening structural characterization of complex mixtures neutral glycosphingolipids (GSLs) is here described. Neutral GSLs purified from human erythrocytes were efficiently separated according the differences carbohydrate chain length by an optimized nano-HPLC protocol flow-through...
In large-scale phosphoproteomics studies, fractionation by strong cation exchange (SCX) or electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) is commonly used to reduce sample complexity, fractionate phosphopeptides from their unmodified counterparts, and increase the dynamic range for phosphopeptide identification. However, these procedures do not succeed separate, both singly multiply phosphorylated peptides due inverse physicochemical characteristics. Hence, depending...
Protein phosphorylation is an important mechanism of cellular signaling, and many proteins are precisely regulated through the interplay stimulatory inhibitory sites. Phosphoproteomics offers great opportunities to unravel this complex interplay, generating a mechanistic understanding vital processes. However, protein substoichiometric and, in particular, peptides carrying multiple sites extremely difficult detect highly mixture abundant nonphosphorylated peptides. Chromatographic methods...
Abstract Matrix‐assisted laser desorption/ionization (MALDI) process of sialoglycoconjugates is generally accompanied by different levels cleavage sialic acid residues and/or dehydration, and decarboxylation reactions. Quantitative densitometry the mouse brain ganglioside (MBG) components separated high‐performance thin layer chromatography (HPTLC) evidenced orcinol staining was a basis to verify composition pattern with respect relative abundances individual in mixture. A systematic mass...
The serine/threonine kinase mammalian target of rapamycin (mTOR) governs growth, metabolism, and aging in response to insulin amino acids (aa), is often activated metabolic disorders cancer. Much known about the regulatory signaling network that encompasses mTOR, but surprisingly few direct mTOR substrates have been established date. To tackle this gap our knowledge, we took advantage a combined quantitative phosphoproteomic interactomic strategy. We analyzed insulin- aa-responsive...
Monosialoganglioside fraction of YAC-1 lymphoma cells was comprehensively analyzed and structurally defined by nano-high-performance liquid chromatography (nanoHPLC) in on-line conjunction with electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QTOF MS). An efficient separation sensitive detection Neu5Gc-containing gangliosides from Neu5Ac-containing analogues for the first time accomplished a single nanoHPLC/ESI-QTOF MS run, as demonstrated mouse hybridoma cell GM3...
Adenoviruses (AdVs) have recently become widely used therapeutic vectors for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine. AdVs are large, nonenveloped viruses with an icosahedral capsid formed from several proteins that encloses double-stranded DNA. These the main components and key players in initial stages of infection by virus particles, so their heterogeneity content must be evaluated to ensure product process consistency. Peptide mapping can provide detailed...
Hydrophobic interaction chromatography (HIC) is a robust standard analytical method to purify proteins while preserving their biological activity. It widely used study post-translational modifications of and drug–protein interactions. In the current manuscript we employed HIC separate proteins, followed by bottom-up LC–MS/MS experiments. We this approach fractionate antibody species comprehensive peptide mapping as well protein complexes in human cells. HIC–reversed-phase (RPC)–mass...
Abstract The chemical composition of the headspace volatiles trapped on active carbon male and female kiwifruit (lowers grown in Iran were analyzed by GC GC/MS. Twenty-seven compounds identified. A large difference flower was not found other than minor quantitative differences. major components valencene (11.7–13.7%), α-cadinol (5.0–7.0%) flourensidiol (7.1%).
Rationale In‐depth characterization of the three capsid viral proteins (VPs 1, 2, and 3) adeno‐associated viruses (AAVs) is immediately needed to ensure consistency in gene therapy products processes. These are typically present at very low concentrations matrices containing high excipients salts. Thus, there a need for convenient methods sample preparation before proteomic analysis. The aim this study was meet by developing fast, reliable approach isolating VPs manner enabling their...
Mitophagy, the autophagic degradation of mitochondria, is an important housekeeping function in eukaryotic cells. Defects mitophagy occur degenerative disorders such as Parkinson’s disease, and decreases levels correlate with aging phenomena. An goal to achieve understanding quality control mechanism(s) that specifically cull defective mitochondrial components or compartments, during mitophagy. Specifically, it not known whether a small cohort molecules will cause ablation entire...
The clearance of malfunctioning mitochondria is an important housekeeping function in respiring eukaryotic cells, and plays a role physiological homeostasis as well the progression maternally inherited late‐onset diseases aging phenomena. Clearance entire mitochondrial comartments thought to occur using endo‐lysosomal system, by specific form autophagic degradation called mitophagy. In standard autophagy assays, such starvation‐induced macroautophagy, no mitophagy observed S. cerevisiae even...