A5038149142- Sarcoma Diagnosis and Treatment
- Reconstructive Surgery and Microvascular Techniques
- Cancer Genomics and Diagnostics
- Inflammasome and immune disorders
- Reconstructive Facial Surgery Techniques
- Adipokines, Inflammation, and Metabolic Diseases
- Extracellular vesicles in disease
- Breast Implant and Reconstruction
- Atherosclerosis and Cardiovascular Diseases
- Facial Nerve Paralysis Treatment and Research
- Cancer-related molecular mechanisms research
- Surgical site infection prevention
- Lipid Membrane Structure and Behavior
- Orthopedic Surgery and Rehabilitation
- Bacterial Genetics and Biotechnology
- Blood properties and coagulation
- Bacteriophages and microbial interactions
- Facial Rejuvenation and Surgery Techniques
- Cancer Diagnosis and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Medical Practices and Rehabilitation
- Ear Surgery and Otitis Media
- Body Image and Dysmorphia Studies
- S100 Proteins and Annexins
- Digital Imaging in Medicine
University Medical Center Freiburg
2016-2025
University of Freiburg
2016-2025
Ludwig-Maximilians-Universität München
2019-2023
LMU Klinikum
2019-2023
Creative Commons
2023
Baker Heart and Diabetes Institute
2014-2019
Sir Charles Gairdner Hospital
2019
Universität Hamburg
2014
Imperial College London
2014
CVPath Institute
2014
Background— The relevance of the dissociation circulating pentameric C-reactive protein (pCRP) to its monomeric subunits (mCRP) is poorly understood. We investigated role conformational changes in vivo. Methods and Results— identified mCRP inflamed human striated muscle, atherosclerotic plaque, infarcted myocardium (rat human) colocalization with inflammatory cells, which suggests a general causal inflammation. This was confirmed rat intravital microscopy lipopolysaccharide-induced...
C-reactive protein (CRP) concentrations rise in response to tissue injury or infection. Circulating pentameric CRP (pCRP) localizes damaged where it leads complement activation and further damage. In-depth knowledge of the pCRP mechanism is essential develop therapeutic strategies minimize injury. Here we demonstrate that by binding cell-derived microvesicles undergoes a structural change without disrupting symmetry (pCRP*). pCRP* constitutes major species human-inflamed allows factor 1q...
Abstract Background C-reactive protein (CRP) represents a routine diagnostic marker of inflammation. Dissociation native pentameric CRP (pCRP) into the monomeric structure (mCRP) liberates proinflammatory features, presumably contributing to excessive immune cell activation via unknown molecular mechanisms. Results In multi-translational study systemic inflammation, we found time- and inflammation-dependent pCRP dissociation mCRP. We were able confirm that mCRP co-localizes with leukocytes...
Protein insertion into the bacterial inner membrane is facilitated by SecYEG or YidC. Although most likely constitutes major integration site, small proteins have been shown to integrate via We show that YidC can also multispanning such as mannitol permease TatC, which had considered be exclusively integrated SecYEG. Only SecA-dependent strictly require for integration, suggests SecA only interact with translocon, but not insertase. Targeting of mediated signal recognition particle (SRP),...
Introduction: C-reactive protein circulates as a pentameric (pCRP). pCRP is well-established diagnostic marker plasma levels rise in response to tissue injury and inflammation. We recently described pro-inflammatory properties of CRP, which are mediated by conformational changes from bioactive isoforms expressing neo-epitopes (pCRP* mCRP). Here, we investigate the role CRP renal ischemia/reperfusion (IRI). Methods: Rat kidneys animals with without intraperitoneally injected were subjected...
Article5 December 2022Open Access Source DataTransparent process A novel phosphocholine-mimetic inhibits a pro-inflammatory conformational change in C-reactive protein Johannes Zeller orcid.org/0000-0002-8122-9063 Department of Plastic and Hand Surgery, University Freiburg Medical Centre, Faculty the Freiburg, Germany Baker Heart Diabetes Institute, Melbourne, Vic., Australia Contribution: Conceptualization, Data curation, Formal analysis, Supervision, Validation, Investigation,...
Microvesicles (MVs) are small cell-derived vesicles, which mainly released by activated cells. They part of a communication network delivering biomolecules, for example, inflammatory molecules, via the blood circulation to remote cells in body. Platelet-derived MVs known induce vascular inflammation. Research on mediators and mechanisms their effects has attracted major interest. We hypothesize that specific lipids inflammation caused platelet-derived MVs. Liquid chromatography electrospray...
Synovial sarcoma account for approximately 10 % of all soft-tissue tumors and occur most frequently in young adults. A specific translocation this induces fusion the SYT gene on chromosome 18 to SSX genes X, leading proliferation tumor cells. The need non-invasive biomarkers indicating recurrence activity disease has sparked research into short non-coding RNA known as microRNA (miRNA). Blood samples patients with active synovial complete remission well healthy donors leiomyosarcoma, MPNST,...
Abstract Tissue damage in burn injury leads to a rapid increase of leukocytes and acute phase reactants. Plasma levels C-reactive protein (CRP) rise within hours after the insult. No deficiency this has been reported humans, suggesting it plays pivotal role innate immunity. CRP circulation is composed five identical subunits [pentameric (pCRP)]. Recently, deposits structurally modified (mCRP) have found inflammatory diseases. Little known about structural change how affects functions. We...
BACKGROUND: CRP (C-reactive protein) is a prototypical acute phase reactant. Upon dissociation of the pentameric isoform (pCRP [pentameric CRP]) into its monomeric subunits (mCRP [monomeric CRP]), it exhibits prothrombotic and proinflammatory activity. Pathophysiological shear rates as observed in aortic valve stenosis (AS) can influence protein conformation function with vWF (von Willebrand factor). Given dissociated important role inflammation pathogenesis AS, we investigated whether...
ABSTRACT Background Myxoid liposarcomas (MLS) can exhibit a disseminated metastatic pattern, necessitating extensive diagnostics during follow‐up. With no tumor markers available, early diagnosis of recurrences and monitoring is difficult. The detection circulating DNA (ctDNA; liquid biopsy) in MLS with the characteristic translocations t(12;16) t(12;22) provide an additional diagnostic. However, due to often very low fraction, distinguishing actual variants from sequencing artifacts remains...
Protein targeting by the signal recognition particle (SRP) and bacterial SRP receptor FtsY requires a series of closely coordinated steps that monitor presence substrate, membrane, vacant translocon. Although influence substrate binding on FtsY-SRP complex formation is well documented, contribution membrane largely unknown. In current study, we found negatively charged phospholipids stimulate formation. Phospholipids act conserved positively amphipathic helix in induce conformational change...
Soft tissue sarcomas (STS) are rare tumors of mesenchymal origin. About 50% patients with STS experience relapse and more than 30% will die within 10 years after diagnosis. In this study we investigated circulating free DNA (cfDNA) tumor‐specific genetic alterations therein (circulating tumor DNA, ctDNA) as diagnostic biomarkers. Plasma concentrations fragmentation cfDNA was analyzed quantitative PCR. Patients ( n = 64) had significantly higher plasma increased when compared to in complete...
BACKGROUND: Liposarcoma constitute about 13% of all soft tissue sarcoma and are associated with a high risk metastases. As the preoperative differentiation between benign malign lipomatous tumors is restricted to magnetic resonance imaging, computed tomography biopsy, we performed miRN A array distinguish dedifferentiated liposarcoma patients from healthy controls lipoma patients. METHODS:Blood samples liposarcoma, were collected. Whole blood RNA was extracted (n= 6) donors 4) analyzed using...
Phagocytosis and the formation of reactive oxygen species (ROS) in phagocytic leukocytes are an effective killing mechanism innate host defense. These cellular processes immunity function a complex interplay with humoral factors. C-reactive protein (CRP) its activated, monomeric isoform (mCRP) has been shown to activate immune cells via classical complement pathway. We investigated complement-dependent effects CRP on neutrophils monocyte subtypes using complement-specific inhibitors by both...
Synovial sarcoma (SS) is a malignant soft tissue tumor of mesenchymal origin that frequently occurs in young adults. Translocation the SYT gene on chromosome 18 to SSX genes X leads formation oncogenic fusion genes, which lead initiation and proliferation cells. The detection quantification circulating DNA (ctDNA) can serve as non-invasive method for diagnostics local or distant recurrence, could improve survival rates due early detection.We developed subtype-specific targeted...
Microvesicles are small vesicles expressing specific antigens from their cells of origin. Elevated levels microvesicles have been shown to be associated with coagulation disorders as well different types malignancies. This study aims evaluate a possible correlation microvesicle subpopulations positive history venous thromboembolism (VTE) in patients soft tissue sarcoma.Annexin V - microvesicles, leukocyte (CD45-positive), platelet (CD61-positive), activated (CD62P-, CD63-positive),...
Facial paralysis has a profound impact on functionality and esthetics of the oral region. In patients with strong skin laxity soft tissue ptosis, functional smile reconstruction is challenging due to accentuated asymmetry at rest. Thus, purpose study was analyze facial symmetry in this patient clientele following combination dynamic reanimation fascial strips for static suspension compared gracilis transfer alone.In 2014, we altered single-stage approach microsurgical significant ptosis by...
Abstract Background Since the emergence of COVID-19 pandemic facecovers have become a common sight. The effect on gaze when looking at faces has not yet been assessed. Objectives aim present study was to investigate any potential differences in eye movement pattern observers exposed images showing face without and with facecover identify if there is truly change identifying (masked) facial features. Methods 64 participants (28 males 36 females) mean [standard deviation] age 31.84 [9.0] years...