A5065023388- Reconstructive Surgery and Microvascular Techniques
- Reconstructive Facial Surgery Techniques
- Facial Nerve Paralysis Treatment and Research
- Adipokines, Inflammation, and Metabolic Diseases
- Sarcoma Diagnosis and Treatment
- Ear Surgery and Otitis Media
- Inflammasome and immune disorders
- Extracellular vesicles in disease
- Atherosclerosis and Cardiovascular Diseases
- Monoclonal and Polyclonal Antibodies Research
- Cancer Genomics and Diagnostics
- Platelet Disorders and Treatments
- Facial Rejuvenation and Surgery Techniques
- Breast Implant and Reconstruction
- Body Contouring and Surgery
- Trigeminal Neuralgia and Treatments
- Cardiac Ischemia and Reperfusion
- Blood properties and coagulation
- Lipoproteins and Cardiovascular Health
- Cell Adhesion Molecules Research
- Organ and Tissue Transplantation Research
- Biomarkers in Disease Mechanisms
- Orthopedic Surgery and Rehabilitation
- Breast Cancer Treatment Studies
- Bone fractures and treatments
University Medical Center Freiburg
2016-2025
University of Freiburg
2016-2025
Creative Commons
2023
Baker Heart and Diabetes Institute
2008-2021
Monash University
2006-2016
The University of Western Australia
2016
Royal Perth Hospital
2016
Wockhardt (United States)
2016
CVPath Institute
2014
Universität Hamburg
2014
Background— The relevance of the dissociation circulating pentameric C-reactive protein (pCRP) to its monomeric subunits (mCRP) is poorly understood. We investigated role conformational changes in vivo. Methods and Results— identified mCRP inflamed human striated muscle, atherosclerotic plaque, infarcted myocardium (rat human) colocalization with inflammatory cells, which suggests a general causal inflammation. This was confirmed rat intravital microscopy lipopolysaccharide-induced...
C-reactive protein (CRP) concentrations rise in response to tissue injury or infection. Circulating pentameric CRP (pCRP) localizes damaged where it leads complement activation and further damage. In-depth knowledge of the pCRP mechanism is essential develop therapeutic strategies minimize injury. Here we demonstrate that by binding cell-derived microvesicles undergoes a structural change without disrupting symmetry (pCRP*). pCRP* constitutes major species human-inflamed allows factor 1q...
Abstract Background C-reactive protein (CRP) represents a routine diagnostic marker of inflammation. Dissociation native pentameric CRP (pCRP) into the monomeric structure (mCRP) liberates proinflammatory features, presumably contributing to excessive immune cell activation via unknown molecular mechanisms. Results In multi-translational study systemic inflammation, we found time- and inflammation-dependent pCRP dissociation mCRP. We were able confirm that mCRP co-localizes with leukocytes...
C-reactive protein (CRP) is a predictor of cardiovascular risk. It circulates as pentamer (pentameric CRP) in plasma. The vivo existence monomeric (m)CRP has been postulated, but its function and source are not clear. We show that mCRP deposited human aortic carotid atherosclerotic plaques healthy vessels. pCRP found neither nor diseased As mCRP, we identify mechanism dissociation to mCRP. report activated platelets, which play central role events, mediate this via lysophosphatidylcholine,...
AimsElevated serum C-reactive protein (CRP) following myocardial infarction (MI) is associated with poor outcomes. Although animal studies have indicated a direct pathogenic role of CRP, the mechanism underlying this remains elusive. Dissociation pentameric CRP (pCRP) into pro-inflammatory monomers (mCRP) may directly link to inflammation. We investigated whether cellular microparticles (MPs) can convert pCRP mCRP and transport MI.
P2Y12 blockade improves patient outcomes after myocardial infarction. As well as antithrombotic effects, anti-inflammatory effects may contribute to this beneficial clinical outcome. Here we aimed identify potential of receptor blockers on monocytes and macrophages. Using flow cytometry, migration assays, chambers RNA microarrays, investigated the adenosine diphosphate (ADP) blood monocytes, THP-1 differentiation -expressing platelets can form aggregates with in circulating blood. Mediated...
Metformin is successfully used in the treatment of cycle disorders and anovulation women with polycystic ovary syndrome (PCOS). No data exact point impact insulin resistance (IR) on metformin's efficacy exist.The objective study was to evaluate early potential effects metformin treatment, their time onset, role IR efficacy.This a prospective randomized, double-blind, placebo-controlled trial.The conducted at University Heidelberg, Germany.The patient population 45 oligo-/anovulatory PCOS...
Upper lip rejuvenation is achieved by a large variety of different treatment approaches that clearly lack unified theoretical background the aging processes upper complex. In this study, histological changes occur in were systematically evaluated for first time.Histological cross-cuts complex 20 individuals two age groups, young (<40 years, n = 10) and old (>80 10), analyzed. The specimens collected during autopsies with no facial injuries. Hematoxylin-eosin elastica van Gieson staining was...
Background: Modern reconstructive surgery allows for radical resection and reconstruction of any scalp tumor. However, a significant number patients are still not treated optimally because incomplete guidelines. Methods: The treatment tumors was documented in 60 over 10-year period. Data regarding tumor type, size, localization; procedure; oncologic, functional, aesthetic outcome; complications were collected analyzed retrospectively. These data correlated to recurrence survival rates....
Beta-amyloid (Aβ) plaques and local inflammation are central to the pathogenesis of Alzheimer's disease. Although an association between circulating pentameric C-reactive protein (pCRP) disease has been reported no pathomechanistic link established. We hypothesized that Aβ induce dissociation pCRP individual monomers (mCRP), which possess strong pro-inflammatory properties not shared with localizing plaques. was incubated generated in vitro non-aggregated Aβ(42) peptide. mCRP found only when...
Introduction: C-reactive protein circulates as a pentameric (pCRP). pCRP is well-established diagnostic marker plasma levels rise in response to tissue injury and inflammation. We recently described pro-inflammatory properties of CRP, which are mediated by conformational changes from bioactive isoforms expressing neo-epitopes (pCRP* mCRP). Here, we investigate the role CRP renal ischemia/reperfusion (IRI). Methods: Rat kidneys animals with without intraperitoneally injected were subjected...
Overactivation of renal sympathetic nervous system and low-grade systemic inflammation are common features hypertension. Renal denervation (RDN) reduces activity in patients with resistant However, its effect on has not been examined. We prospectively investigated the RDN monocyte activation uncontrolled hypertension scheduled for RDN. Ambulatory blood pressure, monocyte, subset inflammatory markers were assessed at baseline, 3 months, 6 months after procedure 42 patients. significantly...
Article5 December 2022Open Access Source DataTransparent process A novel phosphocholine-mimetic inhibits a pro-inflammatory conformational change in C-reactive protein Johannes Zeller orcid.org/0000-0002-8122-9063 Department of Plastic and Hand Surgery, University Freiburg Medical Centre, Faculty the Freiburg, Germany Baker Heart Diabetes Institute, Melbourne, Vic., Australia Contribution: Conceptualization, Data curation, Formal analysis, Supervision, Validation, Investigation,...
Objective— Therapeutic anticoagulation is widely used, but limitations in efficacy and bleeding complications cause an ongoing search for new agents. However, with agents developed it seems to be inherent problem that increased efficiency accompanied by increase complications. We investigate whether targeting of anticoagulants activated platelets provides a means overcome this association potency bleeding. Methods Results— Ligand-induced binding sites (LIBS) on fibrinogen/fibrin-binding...