A5000270741- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Sepsis Diagnosis and Treatment
- Complement system in diseases
- Cardiac Arrest and Resuscitation
- Immune Response and Inflammation
- S100 Proteins and Annexins
- Innovations in Medical Education
- Traumatic Brain Injury and Neurovascular Disturbances
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Sulfur Compounds in Biology
- Anatomy and Medical Technology
- Inflammation biomarkers and pathways
- Liver Disease and Transplantation
- Neurosurgical Procedures and Complications
- Neonatal Respiratory Health Research
- Venous Thromboembolism Diagnosis and Management
- Blood disorders and treatments
- Pancreatic function and diabetes
- Cardiac electrophysiology and arrhythmias
- Respiratory Support and Mechanisms
- Neuroscience and Neuropharmacology Research
- Alcohol Consumption and Health Effects
- Inflammasome and immune disorders
University Hospital Ulm
2017-2025
Helmholtz-Institute Ulm
2020-2025
German Red Cross
2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2022-2023
Universitätsklinikum Erlangen
2022-2023
Universität Ulm
2015-2023
German National Academic Foundation
2016
Abstract Background C-reactive protein (CRP) represents a routine diagnostic marker of inflammation. Dissociation native pentameric CRP (pCRP) into the monomeric structure (mCRP) liberates proinflammatory features, presumably contributing to excessive immune cell activation via unknown molecular mechanisms. Results In multi-translational study systemic inflammation, we found time- and inflammation-dependent pCRP dissociation mCRP. We were able confirm that mCRP co-localizes with leukocytes...
A sufficient response of neutrophil granulocytes stimulated by interleukin (IL)-8 is vital during systemic inflammation, for example, in sepsis or severe trauma. Moreover, IL-8 clinically used as biomarker inflammatory processes. However, the effects on cellular key regulators properties such intracellular pH (pH<sub>i</sub>) dependence ion transport proteins and inflammation remain to be elucidated. Therefore, we investigated detail fundamental changes...
The complement and neutrophil defence systems, as major components of innate immunity, are activated during inflammation infection. For migration to the inflamed region, we hypothesized that activation product C5a induces significant changes in cellular morphology before chemotaxis. Exposure human neutrophils dose- time-dependently resulted a rapid receptor-1 (C5aR1)-dependent shape change, indicated by enhanced flow cytometric forward-scatter area values. Similar were observed after...
Hand-held devices have revolutionized communication and education in the last decade. Consequently, mobile learning (m-learning) has become popular among medical students. Nevertheless, there are relatively few studies assessing students' outcomes using m-learning devices. This observational study presents an anatomy tool (eMed-App), application developed to accompany seminar support self-directed of skeletal system. Questionnaire data describe where, how frequently, why students used app....
Complement activation in the diseases paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS) results cytolysis fatal thrombotic events, which are largely refractory to anticoagulation and/or antiplatelet therapy. Anticomplement therapy, however, efficiently prevents events PNH aHUS, but underlying mechanisms remain unresolved. We show that complement-mediated hemolysis whole blood induces platelet similarly by adenosine 5'-diphosphate (ADP). Blockage of C3 or...
Abstract During sepsis, excessive activation of the complement system with generation anaphylatoxin C5a results in profound disturbances crucial neutrophil functions. Moreover, because activity is highly dependent on intracellular pH (pHi), we propose a direct mechanistic link between and pHi. In this article, demonstrate that vitro exposure human neutrophils to significantly increased pHi by selective sodium/hydrogen exchanger. Upstream signaling C5a-mediated alkalinization was C5aR1,...
Cardiac surgery and the associated ischemia-reperfusion injury trigger an inflammatory response, which, in turn, can contribute to organ damage, prolonged hospitalization, mortality. Therefore, present study performed comprehensive monitoring of neutrophil-related inflammation patients who underwent aortic valve surgery, including extracorporeal circulation. Neutrophil-related inflammation, as well alterations cellular physiology, phenotype, function, were analyzed by flow cytometry, ELISA,...
Trauma-induced hemorrhagic shock (HS) plays a decisive role in the development of immune, coagulation, and organ dysfunction often resulting poor clinical outcome. Imbalanced complement activation is intricately associated with molecular danger response damage after HS. Thus, inhibition central component C3 as turnstile both inflammation coagulation hypothesized rational strategy to improve course HS.Applying intensive care conditions, anaesthetized, monitored, protectively ventilated...
Evidence is emerging that systemic inflammation after trauma drives structural and functional impairment of cardiomyocytes leads to cardiac dysfunction, thus worsening the outcome polytrauma patients. This study investigates molecular changes in heart tissue 4 h multiple injuries with additional hemorrhagic shock using a clinically relevant rodent model polytrauma. We determined mediators (keratinocyte chemoattractant, macrophage chemotactic protein 1), activated complement component C3a...
Cystic fibrosis (CF) is a monogenetic disease caused by an impairment of the cystic transmembrane conductance regulator (CFTR). CF affects multiple organs and associated with acute chronic inflammation. In 2020, Elexacaftor-Tezacaftor-Ivacaftor (ETI) was approved to enhance restore remaining CFTR functionality. This study investigates cellular innate immunity, focus on neutrophil activation phenotype, comparing healthy volunteers patients before (T1, n = 13) after six months (T2, 11) ETI...
Platelet-activating factor (PAF) is an important mediator of the systemic inflammatory response. In case sepsis, proper activation and function neutrophils as first line cellular defense are based on a well-balanced physiological However, little known about role PAF in changes during sepsis. Therefore, this study investigates reaction patterns induced by with focus membrane potential (MP), intracellular pH, swelling under pathophysiological conditions hypothesizes that PAF-mediated response...
Sodium thiosulfate (Na2S2O3), an H2S releasing agent, was shown to be organ-protective in experimental hemorrhage. Systemic inflammation activates immune cells, which turn show cell type-specific metabolic plasticity with modifications of mitochondrial respiratory activity. Since can dose-dependently stimulate or inhibit respiration, we investigated the effect Na2S2O3 on metabolism a blinded, randomized, controlled, long-term, porcine model hemorrhage and resuscitation. For this purpose,...
Many Anglo-American universities have undertaken a paradigm shift in how the dissection of human material is approached, such that students are encouraged to learn about lives body donors, and respectfully "personalize" them as beings, rather than treating specimens anonymous cadavers. For purposes this study, provision limited personal information regarding life donor will be referred "personalization" donors. At time, it unknown whether personalization donors can translated into...
Severe tissue trauma-induced systemic inflammation is often accompanied by evident or occult blood-organ barrier dysfunctions, frequently leading to multiple organ dysfunction. However, it unknown whether specific molecules are shed into the circulation early after trauma as potential indicators of an initial The release molecule junctional adhesion molecule-1 (JAM-1) was investigated in plasma C57BL/6 mice 2 h experimental mono- and polytrauma well patients (ISS ≥ 18) during a 10-day...
Studying innate immunity in humans is crucial for understanding its role the pathophysiology of systemic inflammation, particularly complex setting sepsis. Therefore, we standardized a step-by-step process from venipuncture to transfer human model system, while closely monitoring inflammatory response up 3 hours. We aimed an animal-free, whole blood sepsis using commercially available, simple use, tubing system. First, analyzed routine clinical parameters, including cell count and gas...
Background . Polymorphonuclear granulocytes (PMN) play a crucial role in host defense. Physiologically, exposure of PMN to the complement activation product C5a results protective response against pathogens, whereas case systemic inflammation, excessive substantially impairs neutrophil functions. To further elucidate inability properly respond C5a, this study investigates cellular membrane potential C5a. Methods Electrophysiological changes and mitochondrial intracellular pH from human...
In a patient with permanent neonatal syndromic diabetes clinically similar to cases ONECUT1 biallelic mutations, we identified disease-causing deletion located upstream of ONECUT1. Through genetic, genomic and functional studies crucial regulatory region acting as an enhancer specifically during pancreatic development. This contains low-frequency variant showing strong association type 2 other glycemic traits, thus extending the contribution this common forms diabetes. Clinical relevance is...
Traumatic brain injury (TBI) remains one of the leading causes death. Because individual nature trauma (brain, circumstances and forces), humans experience TBIs. This makes it difficult to generalise therapies. Clinical management issues such as whether intracranial pressure (ICP), cerebral perfusion (CPP) or decompressive craniectomy improve patient outcome remain partly unanswered. Experimental drug approaches for treatment secondary (SBI) have not found clinical application. The complex,...