A5083481136- Complement system in diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Blood groups and transfusion
- Sepsis Diagnosis and Treatment
- S100 Proteins and Annexins
- Neonatal Respiratory Health Research
- Immune cells in cancer
- Platelet Disorders and Treatments
- Tryptophan and brain disorders
- Diabetes and associated disorders
- Clostridium difficile and Clostridium perfringens research
- Respiratory viral infections research
- Adipokines, Inflammation, and Metabolic Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Cystic Fibrosis Research Advances
- Adolescent and Pediatric Healthcare
- Vasculitis and related conditions
- Phagocytosis and Immune Regulation
- Hemoglobinopathies and Related Disorders
- Renal Diseases and Glomerulopathies
- Streptococcal Infections and Treatments
- Botulinum Toxin and Related Neurological Disorders
Universität Ulm
2022-2025
University Hospital Ulm
2021-2024
A sufficient response of neutrophil granulocytes stimulated by interleukin (IL)-8 is vital during systemic inflammation, for example, in sepsis or severe trauma. Moreover, IL-8 clinically used as biomarker inflammatory processes. However, the effects on cellular key regulators properties such intracellular pH (pH<sub>i</sub>) dependence ion transport proteins and inflammation remain to be elucidated. Therefore, we investigated detail fundamental changes...
Complement activation in the diseases paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS) results cytolysis fatal thrombotic events, which are largely refractory to anticoagulation and/or antiplatelet therapy. Anticomplement therapy, however, efficiently prevents events PNH aHUS, but underlying mechanisms remain unresolved. We show that complement-mediated hemolysis whole blood induces platelet similarly by adenosine 5'-diphosphate (ADP). Blockage of C3 or...
Cardiac surgery and the associated ischemia-reperfusion injury trigger an inflammatory response, which, in turn, can contribute to organ damage, prolonged hospitalization, mortality. Therefore, present study performed comprehensive monitoring of neutrophil-related inflammation patients who underwent aortic valve surgery, including extracorporeal circulation. Neutrophil-related inflammation, as well alterations cellular physiology, phenotype, function, were analyzed by flow cytometry, ELISA,...
Introduction Most trauma patients require intensive care treatment and are susceptible to developing persistent inflammation immunosuppression, potentially leading multi organ dysfunction syndrome (MODS) dependence on long term facilities. T cells undergo changes in numbers function post trauma. cell polytraumatized was characterized using functional immunomonitoring predict individual clinical outcome. Moreover, the potential reverse Interleukin (IL)-7 examined. Methods Blood samples were...
Cystic fibrosis (CF) is a monogenetic disease caused by an impairment of the cystic transmembrane conductance regulator (CFTR). CF affects multiple organs and associated with acute chronic inflammation. In 2020, Elexacaftor-Tezacaftor-Ivacaftor (ETI) was approved to enhance restore remaining CFTR functionality. This study investigates cellular innate immunity, focus on neutrophil activation phenotype, comparing healthy volunteers patients before (T1, n = 13) after six months (T2, 11) ETI...
Platelet-activating factor (PAF) is an important mediator of the systemic inflammatory response. In case sepsis, proper activation and function neutrophils as first line cellular defense are based on a well-balanced physiological However, little known about role PAF in changes during sepsis. Therefore, this study investigates reaction patterns induced by with focus membrane potential (MP), intracellular pH, swelling under pathophysiological conditions hypothesizes that PAF-mediated response...
Abstract The role of the complement system in schizophrenia (Sz) is inconclusive due to heterogeneity disease and study designs. Here, we assessed levels activation products functionality classical pathway acutely ill unmedicated Sz patients at baseline after 6 weeks treatment versus matched controls. included analyses terminal complex (sTCC) C5a plasma from 96 controls by enzyme-linked immunosorbent assay. Sub-group analysis serum was conducted for measurement C4 component activity (28 24...
The cellular and fluid phase-innate immune responses of many diseases predominantly involve activated neutrophil granulocytes complement factors. However, a comparative systematic analysis the early impact key soluble cleavage products, including anaphylatoxins, on granulocyte function is lacking. Neutrophil activity was monitored by flow cytometry regarding (electro-)physiology, activity, changes in surface expression activation markers. study revealed no major effects induced C3a or C4a...
The binary C2 toxin of Clostridium (C.) botulinum consists two non-linked proteins, the enzyme subunit C2I and separate binding/transport C2II. To exhibit toxic effects on mammalian cells, proteolytically activated C2II (C2IIa) forms barrel-shaped heptamers that bind to carbohydrate receptors which are present all cell types. binds C2IIa complexes internalized via receptor-mediated endocytosis. In acidified endosomal vesicles, change their conformation insert as pores into membranes. These...
Neutrophils provide rapid and efficient defense mechanisms against invading pathogens. Upon stimulation with proinflammatory mediators, including complement factors bacterial peptides, neutrophils respond changes in their membrane potential, intracellular pH, cellular size. This study provides an approach to quantify these important simultaneously using multiparametric flow cytometry, thereby revealing a typical sequence of neutrophil activation consisting depolarization, alkalization,...
The complement system plays a crucial role in host defense, homeostasis, and tissue regeneration bridges the innate adaptive immune systems. Although genetic variants C2 (c.839_849+17del; p.(Met280Asnfs*5)) C8B (c.1625C&gt;T; p.(Thr542Ile)) are known individually, here, we report on patient carrying their combination heterozygous form. presented with reduced general condition suffers from wide variety of autoimmune diseases. While no disease-specific autoantibodies could be detected,...