A5088445750- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Drug Transport and Resistance Mechanisms
- RNA modifications and cancer
- Neuropeptides and Animal Physiology
- Mitochondrial Function and Pathology
- Biochemical and Molecular Research
- Mass Spectrometry Techniques and Applications
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Carbohydrate Chemistry and Synthesis
- Bioactive Compounds and Antitumor Agents
- Plant biochemistry and biosynthesis
- Hearing, Cochlea, Tinnitus, Genetics
- Pharmacogenetics and Drug Metabolism
- Sociology and Education Studies
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Metabolism and Genetic Disorders
- CAR-T cell therapy research
- Plant Water Relations and Carbon Dynamics
- Vestibular and auditory disorders
- Analytical Chemistry and Chromatography
- Genetic and Kidney Cyst Diseases
- Porphyrin and Phthalocyanine Chemistry
University of Freiburg
2016-2025
Georg Simon Ohm University of Applied Sciences Nuremberg
2014
University of Applied Sciences Biberach
2009-2011
University of Tübingen
1997-2009
Universitätsklinikum Tübingen
2004-2009
University Children's Hospital Tübingen
2005-2008
Benemérita Universidad Autónoma de Puebla
2007
Harvard University
2003
Science Council
2003
Humboldt-Universität zu Berlin
2003
Hypothesis: Local application of dexamethasone-21-dihydrogen-phosphate (Dex-P) to the round window (RW) membrane guinea pigs produces a substantial basal-apical concentration gradient in scala tympani (ST) perilymph. Background: In recent years, intratympanically applied glucocorticoids are increasingly being used for treatment inner ear disease. Although measurements intracochlear concentrations after RW exist, there is limited information on distribution these drugs fluids. It has been...
The cellular responses induced by mitochondrial dysfunction remain elusive. Intrigued the lack of almost any glomerular phenotype in patients with profound renal ischemia, we comprehensively investigated primary sources energy podocytes. Combining functional measurements oxygen consumption rates, metabolite analysis, and determination density podocytes vivo, demonstrate that anaerobic glycolysis fermentation glucose to lactate represent key source Under physiological conditions, could detect...
Abstract The analysis of myo -inositol phosphates (InsPs) and pyrophosphates (PP-InsPs) is a daunting challenge due to the large number possible isomers, absence chromophore, high charge density, low abundance, instability esters anhydrides. Given their importance in biology, an analytical approach follow understand this complex signaling hub desirable. Here, capillary electrophoresis (CE) coupled electrospray ionization mass spectrometry (ESI-MS) implemented analyze mixtures InsPs PP-InsPs...
Metabolism in cells adapts quickly to changes nutrient availability and cellular differentiation status, including growth conditions cell culture settings. The last decade saw a vast increase three-dimensional (3D) techniques, engendering spheroids organoids. These methods were established improve comparability vivo situations, processes modalities. How far mimic metabolism, however, remains enigmatic. Here, our knowledge, we compare for the first time metabolic fingerprints between grown as...
Abstract Murine double minute 2 (MDM2) negatively regulates the activity of tumor suppressor protein p53. Nutlin-3 is a MDM2 inhibitor under preclinical investigation as nongenotoxic activator p53 pathway for cancer therapy. Here, nutlin-3 was evaluated its alone or in combination with established chemotherapeutic drugs antitumor action chemosensitive and chemoresistant neuroblastoma rhabdomyosarcoma cell lines. Effects single treatment were much more pronounced wild-type lines (IC50s...
Caprazamycins are potent anti-mycobacterial liponucleoside antibiotics isolated from Streptomyces sp. MK730-62F2 and belong to the translocase I inhibitor family. Their complex structure is derived 5'-(beta-O-aminoribosyl)-glycyluridine comprises a unique N-methyldiazepanone ring. The biosynthetic gene cluster has been identified, cloned, sequenced, representing first of inhibitor. Sequence analysis revealed presence 23 open reading frames putatively involved in export, resistance,...
Abstract Cathepsin D (CTSD) is a lysosomal protease and marker of poor prognosis in breast cancer. However, the cells responsible for this association function CTSD cancer are still incompletely understood. By using conditional knockout mouse crossed to transgenic MMTV-PyMT model we demonstrate that deficiency mammary epithelium, but not myeloid cells, blocked tumor development cell-autonomous manner. We show lack impaired mechanistic Target Rapamycin Complex 1 (mTORC1) signaling induced...
Article5 December 2022Open Access Source DataTransparent process A novel phosphocholine-mimetic inhibits a pro-inflammatory conformational change in C-reactive protein Johannes Zeller orcid.org/0000-0002-8122-9063 Department of Plastic and Hand Surgery, University Freiburg Medical Centre, Faculty the Freiburg, Germany Baker Heart Diabetes Institute, Melbourne, Vic., Australia Contribution: Conceptualization, Data curation, Formal analysis, Supervision, Validation, Investigation,...
Ring A (3-dimethylallyl-4-hydroxybenzoic acid) is a structural moiety of the aminocoumarin antibiotics novobiocin and clorobiocin. In present study, prenyltransferase involved in biosynthesis this was identified from clorobiocin producer ( Streptomyces roseochromogenes ), overexpressed, purified. It soluble, monomeric 35-kDa protein, encoded by gene cloQ . 4-Hydroxyphenylpyruvate dimethylallyl diphosphate were as substrates enzyme, with K m values determined 25 35 μM, respectively....
Preparations containing willow bark extract are popular herbal remedies, but they mostly standardised with respect to only one compound (usually salicin). RP-HPLC using a C18-column eluted water:methanol: tetrahydrofuran and coupled electrospray triple-quadrupole MS MS/MS was used for the characterisation of dried extracts Salix spp. identification their constituents. Comparison reference substances led 13 compounds (saligenin, salicylic acid, salicin, isosalicin, picein, salidroside,...
Modified nucleosides are formed post-transcriptionally in RNA. In cancer disease, the cell turnover and thus RNA metabolism is increased, yielding higher concentrations of excreted modified nucleosides. presented study, urinary ribonucleosides were used to differentiate between breast patients healthy volunteers. The extracted from urine samples using affinity chromatography subsequently analyzed via liquid ion trap mass spectrometry (LC-IT-MS). peak areas related internal standard...
Abstract Background Breast cancer belongs to the most frequent and severe types in human. Since excretion of modified nucleosides from increased RNA metabolism has been proposed as a potential target pathogenesis breast cancer, aim present study was elucidate predictability by means urinary excreted nucleosides. Methods We analyzed urine samples 85 women respective healthy controls assess metabolic profiles comprehensive bioinformatic approach. All included nucleosides/ribosylated...
Douglas fir (Pseudotsuga menziesii) is a conifer species that stores large amounts of terpenoids, mainly monoterpenoids in resin ducts various tissues. The effects drought on stored leaf terpenoid concentrations trees are scarcely studied and published data partially controversial, since reduced, unaffected or elevated contents due to have been reported. Even less known the effect root terpenoids. In present work, we investigated reduced water availability content roots needles seedlings....
The skin needs to balance tolerance of colonizing microflora with rapid detection potential pathogens. Flexible response mechanisms would seem most suitable accommodate the dynamic challenges effective antimicrobial defense and restoration tissue homeostasis. Here, we dissected macrophage-intrinsic microenvironmental cues that tune macrophage signaling in localized infection opportunistic pathogen Staphylococcus aureus. Early infection, cytokine granulocyte-macrophage colony-stimulating...
In the biosynthetic gene cluster of aminocoumarin antibiotic clorobiocin, small ORF cloY encodes a 71 aa protein which shows significant sequence similarity to mbtH from mycobactin Mycobacterium tuberculosis. mbtH-like genes are frequently found in clusters peptide antibiotics and siderophores, but their function has remained enigmatic. recent publication it been suggested that these may have no for secondary metabolite biosynthesis. An in-frame deletion clorobiocin now carried out. When...
Abstract Background The anticancer drug Ukrain (NSC-631570) which has been specified by the manufacturer as semisynthetic derivative of Chelidonium majus L. alkaloid chelidonine and alkylans thiotepa was reported to exert selective cytotoxic effects on human tumour cell lines in vitro . Few clinical trials suggest beneficial treatment cancer. Aim present study elucidate importance apoptosis induction for antineoplastic activity Ukrain, define molecular mechanism its identify active...
Hypothesis To determine whether intracochlearly applied dexamethasone will lead to better control of drug levels, higher peak concentrations, and lower base-to-apex concentration gradients in the scala tympani (ST) guinea pig than after intratympanic (round window [RW]) application. Background Local application drugs RW results substantial variation intracochlear levels significant ST. Methods Two microliters dexamethasone-phosphate (10 mg/ml) were injected into ST either through membrane,...