A5005254890- Phytase and its Applications
- Trace Elements in Health
- Pancreatic function and diabetes
- Cellular transport and secretion
- Lysosomal Storage Disorders Research
- Calcium signaling and nucleotide metabolism
- Pancreatitis Pathology and Treatment
- Iron Metabolism and Disorders
- Protein Kinase Regulation and GTPase Signaling
- RNA Research and Splicing
- Parathyroid Disorders and Treatments
- RNA and protein synthesis mechanisms
- Retinal Development and Disorders
- RNA modifications and cancer
- Plant Micronutrient Interactions and Effects
- Trypanosoma species research and implications
- Endoplasmic Reticulum Stress and Disease
- Photochromic and Fluorescence Chemistry
- Plant nutrient uptake and metabolism
- Biochemical and Molecular Research
- Bone and Dental Protein Studies
- Galectins and Cancer Biology
- Ion Transport and Channel Regulation
University of Freiburg
2018-2023
Abstract The analysis of myo -inositol phosphates (InsPs) and pyrophosphates (PP-InsPs) is a daunting challenge due to the large number possible isomers, absence chromophore, high charge density, low abundance, instability esters anhydrides. Given their importance in biology, an analytical approach follow understand this complex signaling hub desirable. Here, capillary electrophoresis (CE) coupled electrospray ionization mass spectrometry (ESI-MS) implemented analyze mixtures InsPs PP-InsPs...
Significance Biological roles of inorganic phosphate require careful regulation its transport across cell membranes, but mechanisms are poorly understood. We demonstrate that a major cellular export protein, XPR1, is regulated by the most “energetic” signaling molecules—1,5-bisdiphosphoinositol 1,2,3,4-tetrakisphosphate (InsP 8 ). derive this conclusion showing reduced XPR1-mediated efflux when InsP synthesis was attenuated genetic or pharmacological techniques; rescued using liposomes to...
Significance Improved understanding of the epitranscriptomic control process will help decipher regulation global gene expression. Here, we demonstrate that cell-signaling inositol pyrophosphate 5-InsP 7 stabilizes mRNAs are normally committed to decay pathways by NUDT3-mediated removal protective 5′ cap. We demonstrated this effect in vitro using recombinant NUDT3. Then, applied pharmacological, genetic, and chemical tools manipulate cellular levels , thereby showing it enhance mRNA...
Inositol pyrophosphates regulate diverse physiological processes; to better understand their functional roles, assessing tissue-specific distribution is important. Here, we profiled inositol pyrophosphate levels in mammalian organs using an originally designed liquid chromatography–mass spectrometry (LC-MS) protocol and discovered that the gastrointestinal tract (GIT) contained highest of diphosphoinositol pentakisphosphate (IP7) its precursor hexakisphosphate (IP6). Although absolute GIT...
5-InsP 7 is an endogenous negative regulator of Na + /K -ATPase.
β-Cells respond directly to the intracellular photochemical release of caged inositol pyrophosphate isomers with modulations oscillations in cytosolic Ca<sup>2+</sup>.
Inositol pyrophosphates constitute a family of hyperphosphorylated signaling molecules involved in the regulation glucose uptake and insulin sensitivity. While our understanding biological roles inositol heptaphosphates (PP-InsP5) has greatly improved, functions octaphosphates ((PP)2-InsP4) have remained unclear. Here we present synthesis two enantiomeric cell-permeant photocaged (PP)2-InsP4 derivatives apply them to study living β-cells. Photorelease naturally occurring isomer...
Abstract The analysis of myo -inositol phosphates (InsPs) and pyrophosphates (PP-InsPs) is a daunting challenge due to the large number possible isomers, absence chromophore, high charge density, low abundance, instability esters anhydrides. Given their importance in biology, an analytical approach follow understand this complex signaling hub highly desirable. Here, capillary electrophoresis (CE) coupled electrospray ionization mass spectrometry (ESI-MS) implemented analyze mixtures InsPs...
Abstract Inositol pyrophosphates (PP-IPs) regulate diverse physiological processes; to better understand their functional roles, assessing tissue-specific distribution is important. Here, we profiled PP-IP levels in mammalian organs using a novel HILIC-MS/MS protocol and discovered that the gastrointestinal tract (GIT) contained highest of IP 7 its precursor 6 . Although absolute GIT diet-dependent, elevated metabolism still exists under dietary regimes devoid exogenous Of major cells,...