A5060346451- Trace Elements in Health
- Iron Metabolism and Disorders
- Chemical Synthesis and Analysis
- Pancreatic function and diabetes
- Drug Transport and Resistance Mechanisms
- Synthesis and Biological Evaluation
- Origins and Evolution of Life
- Autophagy in Disease and Therapy
- Bioactive Compounds and Antitumor Agents
- Organophosphorus compounds synthesis
- Lysosomal Storage Disorders Research
- Diverse Education Studies and Reforms
- Chronic Lymphocytic Leukemia Research
- Click Chemistry and Applications
- Metalloenzymes and iron-sulfur proteins
- Immunodeficiency and Autoimmune Disorders
- Photoreceptor and optogenetics research
- Calcium signaling and nucleotide metabolism
- Platelet Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Advanced biosensing and bioanalysis techniques
- Photosynthetic Processes and Mechanisms
University of Freiburg
2019-2023
Abstract The analysis of myo -inositol phosphates (InsPs) and pyrophosphates (PP-InsPs) is a daunting challenge due to the large number possible isomers, absence chromophore, high charge density, low abundance, instability esters anhydrides. Given their importance in biology, an analytical approach follow understand this complex signaling hub desirable. Here, capillary electrophoresis (CE) coupled electrospray ionization mass spectrometry (ESI-MS) implemented analyze mixtures InsPs PP-InsPs...
Technical challenges have to date prevented a complete profiling of the levels myo-inositol phosphates (InsPs) and pyrophosphates (PP-InsPs) in mammalian tissues. Here, we deployed capillary electrophoresis mass spectrometry identify record InsPs PP-InsPs several tissues obtained from wild type mice newly created PPIP5K2 knockout strain. We observe that mouse colon harbours unusually high PP-InsPs. Additionally, PP-InsP profile is considerably more complex than previously reported for animal...
Abstract Condensed phosphates may exist as linear, cyclic or branched structures. Due to their important role in nature, linear polyphosphates have been well studied. In contrast, (ultraphosphates) remain largely uncharacterised, because they were already described 1950 exceedingly unstable the presence of water, epitomized antibranching-rule. This rule lacks experimental backup, since, best our knowledge, no rational synthesis defined ultraphosphates is known. Consequently, detailed studies...
T cell-independent (TI) B cell response is critical for the early protection against pathogen invasion. The regulation and activation of Bruton's tyrosine kinase (Btk) known as a pivotal step antigen receptor (BCR) signaling in TI humoral immunity, observed patients with X-linked agammaglobulinemia (XLA) experiencing high incidence encapsulated bacterial infections. However, key questions remain to whether well-established canonical BCR pathway sufficient regulate activity Btk. Here, we find...
Inositol pyrophosphates (PP-InsPs) are an important group of intracellular signaling molecules. Derived from inositol phosphates (InsPs), these molecules feature the presence at least one energetic pyrophosphate moiety on myo-inositol ring. They exist ubiquitously in eukaryotes and operate as metabolic messengers surveying phosphate homeostasis, insulin sensitivity, cellular energy charge. Owing to absence a chromophore metabolites, very high charge density, low abundance, their analysis...
Inositol pyrophosphates (PP-InsPs) are energetic signaling molecules with important functions in mammals. As their biosynthesis depends on ATP concentration, PP-InsPs tightly connected to cellular energy homeostasis. Consequently, an increasing number of studies involve metabolic disorders, such as type 2 diabetes, aspects tumorigenesis, and hyperphosphatemia. Research conducted yeast suggests that the PP-InsP pathway is activated response reactive oxygen species (ROS). However, precise...
β-Cells respond directly to the intracellular photochemical release of caged inositol pyrophosphate isomers with modulations oscillations in cytosolic Ca<sup>2+</sup>.
A tunable chemoenzymatic strategy provides access to magic spot nucleotides and analogues in gram-scale.
Abstract Technical challenges have to date prevented a complete profiling of the levels myo-inositol phosphates (InsPs) and pyrophosphates (PP-InsPs) in mammalian tissues. Here, we deployed capillary electrophoresis mass spectrometry identify record InsPs PP-InsPs several tissues obtained from wild type mice newly-created PPIP5K2 knockout strain. We observe that mouse colon harbours unusually high PP-InsPs. Additionally, PP-InsP profile is considerably more complex than previously reported...
Inositol pyrophosphates (PP-InsPs) are an important group of intracellular signaling molecules. Derived from inositol phosphates (InsPs), these molecules feature the presence at least one energetic pyrophosphate moiety on myo-inositol ring. They exist ubiquitously in eukaryotes and operate as metabolic messengers surveying phosphate homeostasis, insulin sensitivity, cellular energy charge. Owing to absence a chromophore metabolites, very high charge density, low abundance, their analysis...
Abstract The analysis of myo -inositol phosphates (InsPs) and pyrophosphates (PP-InsPs) is a daunting challenge due to the large number possible isomers, absence chromophore, high charge density, low abundance, instability esters anhydrides. Given their importance in biology, an analytical approach follow understand this complex signaling hub highly desirable. Here, capillary electrophoresis (CE) coupled electrospray ionization mass spectrometry (ESI-MS) implemented analyze mixtures InsPs...
1 Abstract Inositol pyrophosphates (PP-InsPs) are energetic signalling molecules with important functions in mammals. As their biosynthesis depends on ATP concentration, PP-InsPs tightly connected to cellular energy homeostasis. Consequently, an increasing number of studies involves metabolic disorders, such as type 2 diabetes, aspects tumorigenesis, and hyperphosphatemia. Research conducted yeast suggests that the PP-InsP pathway is activated response reactive oxygen species (ROS). However,...
<p>Our manuscript explores the uncharted territory of linear branched ultraphosphates by developing and combining synthesis, purification, analysis, computational approaches. This group condensed phosphates as true constitutional isomers polyphosphates was believed to be exceedingly unstable. notion is proven wrong we provide first evidence their rich chemistry (rearrangements), biology (interaction with enzymes) potential in prebiotic phosphorylation chemistry. </p>
Our manuscript explores the uncharted territory of linear branched ultraphosphates by developing and combining synthesis, purification, analysis, computational approaches. This group condensed phosphates as true constitutional isomers polyphosphates was believed to be exceedingly unstable. notion is proven wrong we provide first evidence their rich chemistry (rearrangements), biology (interaction with enzymes) potential in prebiotic phosphorylation chemistry.