A5020755469- Skin and Cellular Biology Research
- Nuclear Structure and Function
- RNA Research and Splicing
- Chemical Synthesis and Analysis
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Bacteriophages and microbial interactions
- Protein purification and stability
- Legume Nitrogen Fixing Symbiosis
- RNA and protein synthesis mechanisms
- Urological Disorders and Treatments
- biodegradable polymer synthesis and properties
- Advanced biosensing and bioanalysis techniques
- Blood properties and coagulation
- Enzyme Structure and Function
- Plant nutrient uptake and metabolism
- Origins and Evolution of Life
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- RNA regulation and disease
- Connexins and lens biology
- Polymer Surface Interaction Studies
- Organophosphorus compounds synthesis
- Photochromic and Fluorescence Chemistry
University of Freiburg
2018-2023
University of Basel
1991-2003
Leiden University
1999
Heidelberg University
1996-1997
European Molecular Biology Laboratory
1996
German Cancer Research Center
1996
Johns Hopkins Medicine
1990-1995
Johns Hopkins University
1990-1995
Novartis (Switzerland)
1995
École Polytechnique Fédérale de Lausanne
1995
The asymmetric unit membrane (AUM) forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening urothelial surface thus preventing cells from rupturing during bladder distention.W e have shown previously that purified bovine GUMS contain four major integral proteins: uroplakins Ia (27 m ) , Ib (28 ma), I1 (16 I11 (47 .This contradicts some previous reports indicating these proteins are absent AUMs several species.Using an improved procedure, we isolated...
Chicken lamin B2, a nuclear member of the intermediate-type filament (IF) protein family, was expressed as full-length in Escherichia coli. After purification, its structure and assembly properties were explored by EM, using both glycerol spraying/low-angle rotary metal shadowing negative staining for preparation, well analytical ultracentrifugation. At first level structural organization, B2 formed "myosin-like" 3.1S dimers consisting 52-nm-long tail flanked at one end two globular heads....
The p62 complex is an oligomeric assembly of O-linked glycoproteins the nuclear pore that interacts with cytosolic transport factors and part machinery for protein import. In this study we have purified from rat liver envelopes analyzed its structure composition. consists four distinct polypeptides (p62, p58, p54, p45) has a mass approximately 234 kDa, calculated hydrodynamic properties supported by chemical cross-linking scanning transmission electron microscopy. These data suggest contains...
The asymmetric unit membrane (AUM) forms numerous plaques covering the apical surface of mammalian urinary bladder epithelium. These contain four major integral proteins called uroplakins Ia, Ib, II and III, which form particles arranged in a well-ordered hexagonal lattice with p6 symmetry constant 16.5 nm. Bovine AUM negatively stained anionic sodium silicotungstate revealed structural detail to 3.1 nm resolution. Correlation averaging resolved each particle into 12 stain-excluding domains...
The eukaryotic proteasome is a barrel-shaped protease complex made up of four seven-membered rings which the outer and inner may contain to seven different α- β-type subunits, respectively. assembly not well understood. We cloned cDNA for HsC8, one known human α-type produced protein in Escherichia coli Recombinant HsC8 forms about 540 kDa consisting double ringlike structures, each ring containing subunits. Such structure has earlier been reported any subunit, but similar formed by...
Abstract Condensed phosphates may exist as linear, cyclic or branched structures. Due to their important role in nature, linear polyphosphates have been well studied. In contrast, (ultraphosphates) remain largely uncharacterised, because they were already described 1950 exceedingly unstable the presence of water, epitomized antibranching-rule. This rule lacks experimental backup, since, best our knowledge, no rational synthesis defined ultraphosphates is known. Consequently, detailed studies...
Using electron microscopy (EM) of negatively stained samples, we have systematically explored the effect aluminum and other multivalent cations on neurofilaments (NFs) in vitro. Interactions these were investigated with bovine, rabbit, rat spinal cord native NFs, 10-nm filaments reconstituted from 68-kDa subunit (NF-L) isolated bovine cord. Our results indicated that, as has been observed classes intermediate (IFs), all caused significant aggregation suggesting that this phenomenon is a...
The fiber cells of the eye lens possess a unique cytoskeletal system known as "beaded-chain filaments" (BFs). BFs consist filensin and phakinin, two recently characterized intermediate filament (IF) proteins. To examine organization assembly these heteropolymeric IFs, we have performed series in vitro polymerization studies transfection experiments. Filaments assembled from purified phakinin exhibit characteristic 19-21-nm periodicity seen many types IFs upon low angle rotary shadowing....
The yeast nucleoporins Nsp1p, Nup49p, and Nup57p form a complex at the nuclear pores which is involved in nucleocytoplasmic transport. To investigate molecular basis underlying formation, recombinant full-length Nup49p carboxyl-terminal domain of lacks FXFG repeat domain, were expressed Escherichia coli. When three purified proteins mixed together, they spontaneously associated to 150-kDa 1:1:1 stoichiometry. In this trimeric complex, fulfills role an organizing center, Nsp1p individually...
The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent used to introduce four phosphates on nucleosides regioselectively one-flask key transformation. obtained are develop capillary electrophoresis UV detection method, enabling nucleotide assignment bacterial extracts.
Inositol pyrophosphates are signaling molecules containing at least one phosphoanhydride bond that regulate a wide range of cellular processes in eukaryotes. With cyclic array phosphate esters and diphosphate groups around myo-inositol, these molecular messengers possess the highest charge density found nature. Recent work deciphering inositol pyrophosphate biosynthesis Arabidopsis revealed important functions nutrient sensing, hormone signaling, plant immunity. However, despite rapid...
Recent advances in mRNA therapeutics demand efficient toolkits for the incorporation of nucleoside analogues into suitable downstream applications. Herein, we report application a versatile enzyme cascade triphosphorylation broad range analogues, including unprotected nucleobases containing chemically labile moieties. Our biomimetic system was preparation triphosphates adenosine, cytidine, guanosine, uridine and non-canonical core structures, as determined by capillary electrophoresis...
Photocages have been successfully applied in cellular signaling studies for the controlled release of metabolites with high spatio-temporal resolution. Commonly, coumarin photocages are activated by UV light and quantum yields uncaging relatively low, which can limit their applications vivo. Here, syntheses, determination photophysical properties, chemical calculations 7-diethylamino-4-hydroxymethyl-thiocoumarin (thio-DEACM) caged adenine nucleotides reported compared to widely used...