A5005786867- Phytase and its Applications
- Plant nutrient uptake and metabolism
- Legume Nitrogen Fixing Symbiosis
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Cellular transport and secretion
- Plant Parasitism and Resistance
- Photosynthetic Processes and Mechanisms
- Insect Resistance and Genetics
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Oral and gingival health research
- Pineapple and bromelain studies
- Insect-Plant Interactions and Control
- RNA and protein synthesis mechanisms
- Enzyme Production and Characterization
- Iron Metabolism and Disorders
- Phytochemical compounds biological activities
- Water Quality Monitoring and Analysis
- Viral Infections and Immunology Research
- Light effects on plants
- Trace Elements in Health
Indian Institute of Science Bangalore
2020-2024
MRC Laboratory for Molecular Cell Biology
2019-2021
University College London
2019-2021
Medical Research Council
2019-2021
University of Bonn
2017-2020
Institute of Crop Science
2020
University of Tübingen
2013-2017
Diphosphorylated inositol polyphosphates, also referred to as pyrophosphates, are important signaling molecules that regulate critical cellular activities in many eukaryotic organisms, such membrane trafficking, telomere maintenance, ribosome biogenesis, and apoptosis. In mammals fungi, two distinct classes of phosphate kinases mediate biosynthesis pyrophosphates: Kcs1/IP6K- Vip1/PPIP5K-like proteins. Here, we report PPIP5K homologs widely distributed plants Arabidopsis thaliana VIH1 VIH2...
In plants, phosphate (P
Inositol phosphates (IPs) comprise a network of phosphorylated molecules that play multiple signaling roles in eukaryotes. IPs synthesis is believed to originate with IP 3 generated from PIP 2 by phospholipase C (PLC). Here, we report mammalian cells PLC-generated are rapidly recycled inositol, and uncover the enzymology behind an alternative “soluble” route IPs. tetrakisphosphate 1-kinase 1 (ITPK1)—found Asgard archaea, social amoeba, plants, animals—phosphorylates I(3)P originating...
Diphospho-myo-inositol polyphosphates, also termed inositol pyrophosphates, are molecular messengers containing at least one high-energy phosphoanhydride bond and regulate a wide range of cellular processes in eukaryotes. While pyrophosphates InsP7 InsP8 present different plant species, both the identity enzymes responsible for synthesis isomer remain unknown. This study demonstrates that Arabidopsis ITPK1 ITPK2 catalyze phosphorylation phytic acid (InsP6) to symmetric 5-InsP7 InsP6 kinase...
Abstract Most Gram-negative phytopathogenic bacteria inject type III effector (T3E) proteins into plant cells to manipulate signaling pathways the pathogen’s benefit. In resistant plants, specialized immune receptors recognize single T3Es or their biochemical activities, thus halting pathogen ingress. However, molecular function and mode of recognition for most remains elusive. Here, we show that Xanthomonas T3E XopH possesses phytase activity, i.e., dephosphorylates phytate ( myo -inositol-...
Inositol polyphosphate binding specificity of the jasmonate receptor is largely determined by F-box protein COI1.
The combinatorial phosphorylation of myo-inositol results in the generation different inositol phosphates (InsPs), which phytic acid (InsP6) is most abundant species eukaryotes. InsP6 also an important precursor higher phosphorylated pyrophosphates (PP-InsPs), such as InsP7 and InsP8, are characterized by a diphosphate moiety ubiquitously found eukaryotic cells. While PP-InsPs regulate various cellular processes animals yeast, their biosynthesis functions plants has remained largely elusive...
The Fusarium oxysporum (Fo) species complex encompasses a diverse range of filamentous plant pathogens, some which provoke systemic infections in angiosperms, leading to vascular wilt disease. Current understanding the Fo pathogenicity mechanisms is primarily centered on plants, where individual isolates exhibit contrasting lifestyles across endophyte-pathogen continuum different host species. Although, were recently shown cause disease non-vascular liverwort Marchantia polymorpha (Mp),...
Phospholipase C (PLC) is well known for its role in animal signaling, where it generates the second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), by hydrolyzing minor phospholipid, phosphatidylinositol 4,5-bisphosphate (PIP2), upon receptor stimulation. In plants, PLC's still unclear, especially because primary targets of both messengers are lacking, i.e. ligand-gated Ca2+ channel protein kinase C, PIP2 levels extremely low. Nonetheless, Arabidopsis genome encodes...
Abstract Diphospho‐ myo ‐inositol phosphates (PP‐InsP y ) are an important class of cellular messengers. Thus far, no method for the transport PP‐InsP into living cells is available. Owing to their high negative charge density, will not cross cell membrane. A strategy circumvent this issue involves generation precursors in which charges masked with biolabile groups. prometabolite would require twelve thirteen groups, need be cleaved by enzymes release parent molecules. Such densely modified...
Inositol phosphate encompasses a large multifaceted family of signalling molecules that originate from the combinatorial attachment groups to inositol ring. To date, four distinct kinases have been identified, namely, IPK, ITPK, IPPK (IP5-2K), and PPIP5K. Although, ITPKs recently identified in archaea, eukaryotes taken advantage these enzymes create sophisticated network based on phosphates. However, it remains largely elusive what fundamental biochemical principles control cascade. Here, we...
Stop codon readthrough (SCR) is the process of continuation translation beyond stop codon, generating protein isoforms with C-terminal extensions. SCR has been observed in viruses, fungi, and multicellular organisms, including mammals. However, largely unexplored plants. In this study, we have analyzed ribosome profiling datasets to identify mRNAs that exhibit Arabidopsis thaliana. Analyses density, coverage, three-nucleotide periodicity reads mRNA region downstream provided strong evidence...
Inositol pyrophosphates are signaling molecules containing at least one phosphoanhydride bond that regulate a wide range of cellular processes in eukaryotes. With cyclic array phosphate esters and diphosphate groups around myo-inositol, these molecular messengers possess the highest charge density found nature. Recent work deciphering inositol pyrophosphate biosynthesis Arabidopsis revealed important functions nutrient sensing, hormone signaling, plant immunity. However, despite rapid...
Jasmonic acid (JA) is a plant hormone that regulates plethora of physiological processes including immunity and development perceived by the F-Box protein, Coronatine-insensitive protein 1 (COI1). The discovery inositol phosphates (InsPs) in COI1 receptor complex highlights their role JAperception. InsPs are phosphate-rich signaling molecules control many aspects physiology. Inositol pyrophosphates (PP-InsPs) diphosphate containing InsP species, which InsP7 InsP8 best characterized ones....
Abstract Inositol pyrophosphates (PP-InsPs) are soluble cellular messengers that integrate environmental cues to induce adaptive responses in eukaryotes. In plants, the biological functions of various PP-InsP species poorly understood, largely due absence canonical enzymes present other The recent identification a new isomer with yet unknown enantiomeric identity, 4/6-InsP 7 eudicot Arabidopsis thaliana , further highlights intricate signalling network employed by plants. abundance land...
ABSTRACT The combinatorial phosphorylation of myo-inositol results in the generation different inositol phosphates (InsP), which phytic acid (InsP 6 ) is most abundant species eukaryotes. InsP also precursor higher phosphorylated forms called pyrophosphates (PP-InsPs), such as 7 and 8 , are characterized by a diphosphate moiety ubiquitously found eukaryotic cells. While PP-InsPs regulate various cellular processes animals yeast, their biosynthesis functions plants has remained largely...
Land plants have evolved sophisticated sensing mechanisms and signalling pathways to adapt phosphate-limited environments. While molecular players contributing these adaptations in flowering been described, how non-vascular bryophytes regulate phosphate (Pi) homeostasis remained largely unknown. In this study, we present findings that both male female of the liverwort Marchantia polymorpha respond altered availability through substantial developmental changes. We show second messenger...
Abstract Diphospho‐ myo ‐inositol phosphates (PP‐InsP y ) are an important class of cellular messengers. Thus far, no method for the transport PP‐InsP into living cells is available. Owing to their high negative charge density, will not cross cell membrane. A strategy circumvent this issue involves generation precursors in which charges masked with biolabile groups. prometabolite would require twelve thirteen groups, need be cleaved by enzymes release parent molecules. Such densely modified...
ABSTRACT In plants, phosphate (P i ) homeostasis is regulated by the interaction of P starvation response transcription factors (PHRs) with stand-alone SPX proteins, which act as sensors for inositol pyrophosphates (PP-InsPs). Recently, ITPK1 was shown to generate PP-InsP InsP 7 from 6 in vitro , but importance this activity signaling remained unknown. Here, we show that uncontrolled accumulation ITPK1-deficient plants accompanied impaired -dependent and 8 synthesis. Reciprocal grafting...
Abstract Targeted protein degradation is essential for physiological development and adaptation to stress. Mammalian INOSITOL PENTAKISPHOSPHATE 2-KINASE (IP5K) HEXAKISPHOSPHATE KINASE 1 (IP6K1) pair generates inositol polyphosphates (InsPs) modulate association/dissociation equilibrium of Cullin RING Ubiquitin E3 ligases (CRLs) on the COP9 signalosome (CSN) platform. Deneddylase activity CSN5 subunit protects cullins from self-ubiquitination ensuring their functional continuity. In plants,...
Inositol pyrophosphates (PP-InsPs) are eukaryote-specific second messengers that regulate diverse cellular processes, including immunity, nutrient sensing, and hormone signaling pathways in plants. These energy-rich exhibit high sensitivity to the phosphate status, suggesting synthesis degradation of PP-InsPs tightly controlled within cells. Notably, molecular basis PP-InsP hydrolysis plants remains largely unexplored. In this study, we report functional characterization MpDDP1, a...