A5012350005- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Plant Parasitism and Resistance
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Virus Research Studies
- Nuclear Physics and Applications
- Radiation Detection and Scintillator Technologies
- Ubiquitin and proteasome pathways
- Polysaccharides and Plant Cell Walls
- Toxoplasma gondii Research Studies
- Legume Nitrogen Fixing Symbiosis
- Radioactivity and Radon Measurements
- Phytase and its Applications
- Nuclear physics research studies
- Particle Detector Development and Performance
- Plant nutrient uptake and metabolism
- Plant and Fungal Interactions Research
- Peptidase Inhibition and Analysis
- Biofuel production and bioconversion
- Forensic and Genetic Research
- Autophagy in Disease and Therapy
- Quantum Chromodynamics and Particle Interactions
- Plant tissue culture and regeneration
- Plant Growth Enhancement Techniques
Regional Centre for Biotechnology
2015-2024
Sylhet MAG Osmani Medical College
2024
ORCID
2023
Homi Bhabha National Institute
2022-2023
Saha Institute of Nuclear Physics
2022-2023
Command Hospital
2022
University of Kalyani
2022
University of Missouri
2008-2018
University of Chittagong
2018
Plant (United States)
2013-2018
Plant resistance proteins detect the presence of specific pathogen effectors and initiate effector-triggered immunity. Few immune regulators downstream have been identified, none which are known virulence targets effectors. We show that Arabidopsis ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1), a positive regulator basal immunity specifically mediated by Toll-interleukin-1 receptor-nucleotide binding-leucine-rich repeat (TIR-NB-LRR) proteins, forms protein complexes with TIR-NB-LRR disease RPS4...
RNA-silencing mechanisms control many aspects of gene regulation including the detection and degradation viral RNA through action of, among others, Dicer-like Argonaute (AGO) proteins. However, extent to which silencing restricts virus host range has been difficult separate from other factors that can affect virus-plant compatibility. Here we show Potato X (PVX) infect Arabidopsis (Arabidopsis thaliana), is normally a nonhost for PVX, if coinfected with second virus, Pepper ringspot virus....
Active resistance to viruses is afforded by plant disease (R) genes, which encode proteins with nucleotide-binding (NB) and leucine-rich repeat (LRR) domains. Upon recognition of pathogen-derived elicitors, these NB-LRR are thought initiate a number signaling pathways that lead pathogen restriction. However, little known about the molecular mechanisms ultimately curtail virus accumulation. Here, we show co-expression protein its cognate elicitor results in an antiviral response inhibits...
The plant immune system must be tightly controlled both positively and negatively to maintain normal growth health. We previously identified SUPPRESSOR OF rps4-RLD1 (SRFR1) as a negative regulator specifically of effector-triggered immunity. SRFR1 is localized in cytoplasmic microsomal compartment the nucleus. Its TPR domain has sequence similarity domains transcriptional repressors other organisms, suggesting that may regulate immunity via control. show here excluding from nucleus prevented...
Limited knowledge currently exists regarding the roles of plant genes and proteins in Agrobacterium tumefaciens-mediated transformation process. To understand host contribution to transformation, we carried out root-based assays identify Arabidopsis mutants that are resistant (rat mutants). date, have identified 126 rat by screening libraries T-DNA insertion using various "reverse genetic" approaches. These disrupt expression numerous categories, including chromatin structural remodeling...
The SUPPRESSOR OF rps4-RLD1 (SRFR1) gene was identified based on enhanced AvrRps4-triggered resistance in the naturally susceptible Arabidopsis accession RLD. No other phenotypic effects were recorded, and extent of SRFR1 involvement regulating effector-triggered immunity unknown. Here we show that mutations Columbia-0 (Col-0) lead to severe stunting constitutive expression defense PR1. These phenotypes temperature-dependent. A cross between srfr1-1 (RLD background) srfr1-4 showed caused by...
Abstract Successful transformation of plants by Agrobacterium tumefaciens requires that the bacterial T-complex actively escorts T-DNA into host's nucleus. VirD2 and VirE2 are virulence proteins on have plant-functional nuclear localization signal sequences may recruit importin α plant for import. In this study, we evaluated involvement seven nine members Arabidopsis thaliana family in transformation. Yeast two-hybrid, bimolecular fluorescence complementation, vitro protein–protein...
Abstract Background Lignin and xylan are important determinants of cell wall structure lignocellulosic biomass digestibility. Genetic manipulations that individually modify either lignin or improve polysaccharide However, the effects their simultaneous modifications have not been explored in a similar context. Here, both individual combinatorial modification was studied by analysing effect on plant properties, biotic stress responses integrity sensing. Results Arabidopsis co-harbouring...
Abstract In a chemical genetics screen we identified the small-molecule [5-(3,4-dichlorophenyl)furan-2-yl]-piperidine-1-ylmethanethione (DFPM) that triggers rapid inhibition of early abscisic acid signal transduction via PHYTOALEXIN DEFICIENT4 (PAD4)- and ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1)-dependent immune signaling mechanisms. However, mechanisms upstream EDS1 PAD4 in DFPM-mediated remain unknown. Here, report DFPM generates an Arabidopsis thaliana accession-specific root growth arrest...
The combinatorial phosphorylation of myo-inositol results in the generation different inositol phosphates (InsPs), which phytic acid (InsP6) is most abundant species eukaryotes. InsP6 also an important precursor higher phosphorylated pyrophosphates (PP-InsPs), such as InsP7 and InsP8, are characterized by a diphosphate moiety ubiquitously found eukaryotic cells. While PP-InsPs regulate various cellular processes animals yeast, their biosynthesis functions plants has remained largely elusive...
Effector-triggered immunity provides plants with strong protection from pathogens. However, this response has the potential to be highly deleterious host and needs tightly controlled. The molecular mechanisms in plant that regulate balance between activation suppression of resistance are not fully understood. Previously, we identified Arabidopsis suppressor rps4-RLD 1 (srfr1) mutants enhanced bacterial effector AvrRps4. These were recessive retained full susceptibility virulent bacteria,...
On infection by pathogens, plants initiate defence responses that are able to curtail locally. These mediated either receptor-like proteins recognize pathogen-associated molecular patterns or the protein products of disease resistance (R) genes. At same time, primary often result in generation signals induce what is known as systemic acquired (SAR), such enhanced on secondary pathogen challenge distal tissues. R protein-mediated SAR induction normally accompanied a type programmed cell death...
Plant NB-LRR proteins confer resistance to multiple pathogens, including viruses. Although the recognition of viruses by is highly specific, previous studies have suggested that activation results in a response targets all infected cell. Using an inducible system activate defenses, we find signaling does not result degradation viral transcripts, but rather prevents them from associating with ribosomes and translating their genetic material. This indicates defense against involves repression...
Agrobacterium tumefaciens transfers DNA to plant cells as a single-stranded molecule (the T-strand) covalently linked VirD2 protein. contains nuclear localization signal sequences that presumably help direct the T-strand nucleus. We identified tomato cDNA clone, DIG3, encodes protein interacts with C-terminal region of VirD2. DIG3 an enzymatically active type 2C serine/threonine phosphatase. Overexpression in tobacco BY-2 protoplasts inhibited import β-glucuronidase-VirD2 fusion Thus, may be...
Bacterial effector proteins secreted into host plant cells manipulate those to the benefit of pathogen, but effector-triggered immunity (ETI) occurs when effectors are recognized by resistance proteins. The RPS4/RRS1 pair recognizes Pseudomonas syringae pv. pisi AvrRps4. AvrRps4 is processed in planta AvrRps4N (133 amino acids), homologous N-termini other including native P. tomato strain DC3000 HopK1, and AvrRps4C (88 acids). Previous data suggested that alone necessary sufficient for...
ABSTRACT The combinatorial phosphorylation of myo-inositol results in the generation different inositol phosphates (InsP), which phytic acid (InsP 6 ) is most abundant species eukaryotes. InsP also precursor higher phosphorylated forms called pyrophosphates (PP-InsPs), such as 7 and 8 , are characterized by a diphosphate moiety ubiquitously found eukaryotic cells. While PP-InsPs regulate various cellular processes animals yeast, their biosynthesis functions plants has remained largely...
Wearing a motorcycle helmet reduces accident fatalities by almost one-third (29 percent). Presently, head injury is leading cause of death in crashes. Riders who don't wear helmets and experience crash are 40 percent more likely to sustain fatal injury. The proposed system includes controlled safety wherein the ignition enabled only when put on stand removed. This also incorporates other features like Global Positioning System; automatic light control Real time display. Such serve as rider...