A5075528090- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Plant Stress Responses and Tolerance
- Plant Parasitism and Resistance
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Hematopoietic Stem Cell Transplantation
- Legume Nitrogen Fixing Symbiosis
- Acute Myeloid Leukemia Research
- Aluminum toxicity and tolerance in plants and animals
- Plant Micronutrient Interactions and Effects
- Plant pathogens and resistance mechanisms
- Immune Cell Function and Interaction
- Acute Lymphoblastic Leukemia research
- Lymphoma Diagnosis and Treatment
- Plant Pathogens and Fungal Diseases
- Immunotherapy and Immune Responses
- Plant Disease Resistance and Genetics
- Chronic Lymphocytic Leukemia Research
- Lung Cancer Treatments and Mutations
- Chronic Myeloid Leukemia Treatments
- Photosynthetic Processes and Mechanisms
- Neutropenia and Cancer Infections
- Horticultural and Viticultural Research
- T-cell and B-cell Immunology
University of Missouri
2016-2025
Plant (United States)
2013-2024
GTx (United States)
2005-2012
Center for Life Sciences
2009
Columbia College - Missouri
2009
University of California, Berkeley
1996-2000
Kiel University
1982-2000
University of California, San Diego
1992-1999
University of Göttingen
1997
Freie Universität Berlin
1994
Sodium (Na + ) at high millimolar concentrations in soils is toxic to most higher plants and severely reduces agricultural production worldwide. However, the molecular mechanisms for plant Na uptake remain unknown. Here, wheat root high-affinity potassium (K transporter HKT1 was shown function as a K -Na cotransporter. High-affinity activated by micromolar concentrations; moreover, (half-activation constant, 2.8 μM ). physiologically detrimental of , accumulation mediated blocked...
Iron, despite being an essential micronutrient, becomes toxic if present at high levels. As a result, plants possess carefully regulated mechanisms to acquire iron from the soil. The ferric reductase defective3 (frd3) mutant of Arabidopsis (Arabidopsis thaliana) is chlorotic and exhibits constitutive expression its uptake responses. Consequently, frd3 mutants overaccumulate iron; yet, paradoxically, phenotypes are due reduction in amount inside leaf cells. FRD3 protein belongs multidrug...
Plant-disease resistance (R) genes mediate the specific recognition of invading pathogens carrying cognate avirulence (avr) determinants. RPS4 is a disease-resistance locus on chromosome 5 Arabidopsis thaliana specifying to strains Pseudomonas syringae pv. tomato expressing avrRps4. We have isolated gene using map-based cloning approach. encodes predicted protein 1217 amino acids that contains an N-terminus with homology intracellular domains Drosophila Toll and mammalian interleukin-1...
Long-distance transport of nitrate requires xylem loading and unloading, a successive process that determines distribution subsequent assimilation efficiency. Here, we report the functional characterization NRT1.8, member transporter (NRT1) family in Arabidopsis thaliana. NRT1.8 is upregulated by nitrate. Histochemical analysis using promoter-beta-glucuronidase fusions, as well situ hybridization, showed expressed predominantly parenchyma cells within vasculature. Transient expression...
Plant defense against pathogens often includes rapid programmed cell death known as the hypersensitive response (HR). Recent genetic studies have demonstrated involvement of a specific mitogen-activated protein kinase (MAPK) cascade consisting three tobacco MAPKs, SIPK, Ntf4 and WIPK, their common upstream MAPK (MAPKK or MEK), NtMEK2. Potential MAPKK kinases (MAPKKKs MEKKs) in this include orthologs Arabidopsis MEKK1 tomato MAPKKKalpha. Activation SIPK/Ntf4/WIPK pathway induces with...
Plant resistance proteins detect the presence of specific pathogen effectors and initiate effector-triggered immunity. Few immune regulators downstream have been identified, none which are known virulence targets effectors. We show that Arabidopsis ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1), a positive regulator basal immunity specifically mediated by Toll-interleukin-1 receptor-nucleotide binding-leucine-rich repeat (TIR-NB-LRR) proteins, forms protein complexes with TIR-NB-LRR disease RPS4...
Abstract The Arabidopsis thaliana AtOPT3 belongs to the oligopeptide transporter (OPT) family, a relatively poorly characterized family of peptide/modified peptide transporters found in archebacteria, bacteria, fungi, and plants. A null mutation resulted embryo lethality, indicating an essential role for development. In this article, we report on isolation phenotypic characterization second mutant line, opt3-2, harboring T-DNA insertion 5′ untranslated region AtOPT3. promoter reduced but...
The wheat root high‐affinity K + transporter HKT1 functions as a sodium‐coupled potassium co‐uptake transporter. At toxic millimolar levels of sodium (Na ), mediates low‐affinity Na uptake while (K ) is blocked. In roots, and inhibition contribute to toxicity. the present study, selectivity among alkali cations expressed in Xenopus oocytes yeast was investigated under various ionic conditions at steady state. data show that highly selective for two physiologically significant cations, over...
Plants under stress from both biotic and abiotic sources produce increased levels of ethylene, which is perceived by ethylene receptors triggers cellular responses further downstream. Protein phosphorylation dephosphorylation were implicated in the regulation induction stresses based on studies using protein kinase phosphatase inhibitors. However, kinase(s) involved remains to be determined. Using a conditional gain-of-function transgenic system, we demonstrate that activation SIPK, tobacco...
Efforts to understand how plants respond aluminum have focused on describing the symptoms of toxicity and elucidating mechanisms tolerance; however, little is known about signal transduction steps that initiate plant's response. Here, we image cortical microtubules quantify plasma-membrane potential in living, root cells intact Arabidopsis seedlings. We show depolymerizes depolarizes membrane, these responses are prevented by calcium channel blockade. Calcium influx might involve glutamate...
K+ is the most abundant cation in cells of higher plants, and it plays vital roles plant growth development. Extensive studies on kinetics uptake roots have shown that mediated by at least two transport mechanisms, one with a high low affinity for K+. However, precise molecular mechanisms from soils into root epidermal remain unknown. In present study we pursued biophysical identification characterization single hairs wheat (Triticum aestivum L.), since constitute an important site nutrient...
The Arabidopsis (Arabidopsis thaliana) disease resistance protein RESISTANCE TO PSEUDOMONAS SYRINGAE4 (RPS4) activates defenses in response to bacterial pathogens expressing avrRps4 a gene-for-gene specific manner. RPS4 gene produces multiple transcripts via alternative splicing of two regular introns flanking exon 3 and cryptic intron within 3. We showed previously that RPS4-mediated requires the combined presence encoding both full-length truncated open reading frames. Here, we demonstrate...
The element boron (B) is an essential plant micronutrient, and B deficiency results in significant crop losses worldwide. maize (Zea mays) tassel-less1 (tls1) mutant has defects vegetative inflorescence development, comparable to the effects of deficiency. Positional cloning revealed that tls1 encodes a protein aquaporin family co-orthologous known channel proteins other species. Transport assays show TLS1 facilitates movement water into Xenopus laevis oocytes. content reduced mutants,...
The plant immune system must be tightly controlled both positively and negatively to maintain normal growth health. We previously identified SUPPRESSOR OF rps4-RLD1 (SRFR1) as a negative regulator specifically of effector-triggered immunity. SRFR1 is localized in cytoplasmic microsomal compartment the nucleus. Its TPR domain has sequence similarity domains transcriptional repressors other organisms, suggesting that may regulate immunity via control. show here excluding from nucleus prevented...
We report the results of a genome-wide analysis transcription in Arabidopsis thaliana after treatment with Pseudomonas syringae pathovar tomato. Our time course RNA-Seq experiment uses over 500 million read pairs to provide detailed characterization response infection both susceptible and resistant hosts. The set observed differentially expressed genes is consistent previous studies, confirming extending existing findings about likely play an important role defense syringae. high coverage...
2′-Deoxyguanosine, 3′-dGMP, 5′-dGMP, d-GpG, or double-stranded DNA were exposed to H2O2 in the presence of Fe2+ under anaerobic conditions aerobic Fe3+, Fe2+, Fe2+/NADH, Fe3+/NADH with and without ethanol. The products enzymatically digested nucleosides, separated by high performance liquid chromatography (HPLC), quantified, characterized HPLC retention time, radiolabeling, UV absorbance spectrometry, NMR, mass spectrometry. 20 products, constituting 78-81% damage, distinguished from...
In patients with adult ALL, substantial progress has been made in the past 20 years. At present a cure rate of 30-40% can be achieved and varies defined subgroups between 10-51%. ALL does not represent an uniform disease but is formed by biologic subentities, which differ their natural history, clinical presentation prognosis. A comprehensive diagnostic examination, including morphology, cytochemistry, immunology, cytogenetic molecular genetic analysis precondition for prognostic assessment...
Abstract Excessive low-affinity Na+ uptake is toxic to the growth of glycophytic plants. Recently, several reports have suggested that interaction between K+ and might represent a key factor in determining tolerance We investigated effects starvation on mechanisms plasma membrane wheat (Triticum aestivum L.) root cortex cells using patch-clamp technique. Unexpectedly, K+starvation seedlings was found enhance magnitude frequency occurrence time-dependent inward-rectifying channel currents (IK...
Abstract High-affinity K+ uptake in plant roots is rapidly up-regulated when withheld and down-regulated resupplied. These processes make important contributions to homeostasis. A cDNA coding for a high-affinity transporter, HKT1, was earlier cloned from wheat (Triticum aestivum L.) functionally characterized. We demonstrate here that both barley (Hordeum vulgare roots, rapid large up-regulation ofHKT1 mRNA levels resulted withdrawn growth media. This effect specific K+; withholding N caused...