A5101745791- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Genetic diversity and population structure
- Insect-Plant Interactions and Control
- Wheat and Barley Genetics and Pathology
- Genetic Mapping and Diversity in Plants and Animals
- Evolution and Genetic Dynamics
- Plant Pathogens and Resistance
- Insect behavior and control techniques
- Plant Molecular Biology Research
- Forest Insect Ecology and Management
- Metaheuristic Optimization Algorithms Research
- Plant and fungal interactions
- Insect Pest Control Strategies
- Plant Genetic and Mutation Studies
- Diptera species taxonomy and behavior
- Insect and Pesticide Research
- Fish Ecology and Management Studies
- Environmental DNA in Biodiversity Studies
- Antioxidant Activity and Oxidative Stress
- Research in Cotton Cultivation
- Wildlife Ecology and Conservation
- Microbial Natural Products and Biosynthesis
Purdue University West Lafayette
2003-2023
Agricultural Research Service
2009-2023
United States Department of Agriculture
2023
State Street (United States)
2008
University of Arkansas at Fayetteville
2002
Texas A&M University
1996-1999
Limited knowledge currently exists regarding the roles of plant genes and proteins in Agrobacterium tumefaciens-mediated transformation process. To understand host contribution to transformation, we carried out root-based assays identify Arabidopsis mutants that are resistant (rat mutants). date, have identified 126 rat by screening libraries T-DNA insertion using various "reverse genetic" approaches. These disrupt expression numerous categories, including chromatin structural remodeling...
We investigated the effect of RNAi-mediated gene silencing 109 Arabidopsis thaliana chromatin-related genes (termed "chromatin genes" hereafter) on Agrobacterium-mediated root transformation. Each RNAi lines contains a single- or low-copy-number insertion hairpin construction that silences endogenous copy target gene. used three standard transient and stable transformation assays to screen 340 independent lines, representing genes, for rat (resistant Agrobacterium transformation) phenotype....
Abstract This article documents the addition of 283 microsatellite marker loci to Molecular Ecology Resources Database. Loci were developed for following species: Agalinis acuta ; Ambrosia artemisiifolia Berula erecta Casuarius casuarius Cercospora zeae‐maydis Chorthippus parallelus Conyza canadensis Cotesia sesamiae Epinephelus acanthistius Ficedula hypoleuca Grindelia hirsutula Guadua angustifolia Leucadendron rubrum Maritrema novaezealandensis Meretrix meretrix Nilaparvata lugens...
To our knowledge, there has been no published technique to produce consistently high-quality slides of somatic chromosomes roses ( Rosa sp.). Therefore, various pretreatments, fixatives, digestions, stains, and maceration squashing methods were tested identify a procedure clear, well-spread from shoot tips. The best results obtained after pretreatment in mixture 0.1% colchicine 0.001 m 8-hydroxyquinoline for 4 h, fixation 2 acetone: 1 acetic acid (v/v) with 2% (w/v) polyvinylpyrrolidone....
SUMMARYMitotic chromosome numbers and measurements were determined for fifteen species of Rosa, subgenera Hulthemia, Platyrhodon, Hesperhodos, Rosa (the latter represented by sections Pimpinellifoliae, Carolinae, Cinnamoneae, Synstylae, Banksianae, Chinenses, Caninae, Laevigatae, Bracteatae). All 2n = 2x 14, 4x 28, or 5x 35, as expected from previously published counts. Similar chromosomes matched in pairs least-squares analysis arm lengths. Arm ratio heterogeneity length used to compare...
Ninety-four microsatellites from enriched genomic libraries of Hessian fly (Hf, Mayetiola destructor [Say]) were localized to 170 cognate clones in an Hf bacterial artificial chromosome (BAC) library. These microsatellite-positive BAC physically mapped polytene chromosomes by fluorescent situ hybridization. The microsatellite loci can be used study the genetic diversity and population structure Hf.
A microsatellite library was prepared from size-selected genomic DNA of Hessian fly (Mayetiola destructor). Approximately 81% recovered clones hybridized with motif-specific probes. Subsequently, 2350 were sequenced. Sixty-two individual flies laboratory strains used to test for reliability and polymorphism in 50 the microsatellites by gel electrophoresis; 18 further tested capillary electrophoresis. Of these, 17 behaved as a polymorphic single locus appropriate population analysis.
Chiasmata constitute one of the cornerstones sexual reproduction in most eukaryotes. They mediate reciprocal genetic exchange between homologues and are essential to proper orientation homologous centromeres meiosis I. As markers recombination, they offer a cytological means mapping. Rather than trying accurately count individual chiasmata, we have examined properties mathematical relationship frequencies nonadorned disomic configurations (ring, rods, univalents) probabilities at which arms...
Motivation: High-throughput methods are beginning to make possible the genotyping of thousands loci in individuals, which could be useful for tightly associating phenotypes candidate loci. Current mapping algorithms cannot handle so many data without building hierarchies framework maps.
The tetraploid (2n = 4x 28) rose 86-7 ( Rosa wichuraiana × R. rugosa rubra ) and its hybrids with the thornless cultivar Basye's Blueberry were analyzed for meiotic configuration frequencies abnormalities. Genomic relationships in these interpreted aid of a model chromosome association tetraploids. closest-fitting solutions indicated 2:2 (AABB) pattern genomic relationships, 65% to 90% all between most closely related genomes. Some optimal transitional “ring4” pattern, which one possible...
Eighteen polymorphic microsatellite loci and 11 single-nucleotide polymorphisms were genotyped in 1,095 individual Hessian fly specimens representing 23 populations from North America, southern Europe, southwest Asia. The genotypes used to assess genetic diversity interrelationship of populations. While phylogenetic analysis indicates that the American most similar Eurasian come east coast United States, distance is least between (Alabama California) (Kazakhstan Spain). Allelic frequency...
Exploration of the biotype structure Hessian fly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae), would improve our knowledge regarding variation in virulence phenotypes and difference genetic background. Microsatellites (simple sequence repeats) single-nucleotide polymorphisms (SNPs) are highly variable markers that widely used population studies. This study developed tested a panel 18 microsatellite 22 SNP to investigate nine fly biotypes: B, C, D, E, GP, L, O, vH9, vH13. The simple...
An experiment was performed to measure the effect of Cereal Yellow-Dwarf Virus (CYDV), strain CYDV-RPV, on gene expression in its insect vector, greenbug aphid ( Schizaphis graminum (Rondani)). RNA sampled three replicates from four treatments (biotypes B and H with or without carried CYDV), at 0, 1, 2, 3, 5, 10, 15 20 days introduction carrier virus-free greenbugs uninfected wheat cv. ‘Newton’. Illumina paired-end sequencing produced 1,840,820,000,000 raw reads that yielded 1,089,950,000...
Salivary proteins are the initial contact between sedentary insect pests and their host plants. It is expected that one or more salivary mediate interaction Hessian fly wheat, in which a feeding site established to benefit of fly. A survey 52 loci annotated as secreted was conducted 384 individuals evenly distributed among eight biotypes (B, C, D, E, GP, L, O, vH9). Amplicons were sequenced with Illumina, sequence reads aligned reference sequences from primers had been designed. Positions...
Meiotic chromosome configuration frequencies at diakinesis and metaphase I were tabulated in diploid induced tetraploid Rosa laevigata Michx., R. roxburghii Tratt., amphidiploids of with banksiae Ait. Numerical analysis suggests up to two-fold length variation between short long arms the diploids, incomplete independence during pairing chiasma formation tetraploids. Since sampled genomes pair homeologously intersubgeneric amphidiploids, they are relatively closely related. The meiotic data...
Abstract Like other cecidomyiid Diptera, Hessian fly has stable S chromosomes and dispensable E that are retained only in the germ line. Amplified fragment length polymorphisms (AFLP), suppressive subtractive hybridization (SSH), fluorescent in-situ (FISH), sequencing were used to investigate similarities differences between chromosomes. More than 99.9% of AFLP bands identical separated ovary somatic tissue, but one band was unique resembled Worf , a non-LTR retrotransposon. Arrayed clones,...