A5052448068- Wheat and Barley Genetics and Pathology
- Insect Resistance and Genetics
- Insect-Plant Interactions and Control
- Genetic diversity and population structure
- Forest Insect Ecology and Management
- Insect symbiosis and bacterial influences
- Plant Disease Resistance and Genetics
- Hymenoptera taxonomy and phylogeny
- Evolution and Genetic Dynamics
- Insect behavior and control techniques
- Insect Pest Control Strategies
- Mycotoxins in Agriculture and Food
- Malaria Research and Control
- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Genetically Modified Organisms Research
- Mosquito-borne diseases and control
- Soybean genetics and cultivation
- Invertebrate Immune Response Mechanisms
- Neurobiology and Insect Physiology Research
- Diptera species taxonomy and behavior
- Plant and fungal interactions
- Glutathione Transferases and Polymorphisms
- Entomopathogenic Microorganisms in Pest Control
- Insect Utilization and Effects
Purdue University West Lafayette
2013-2024
Agricultural Research Service
2011-2024
United States Department of Agriculture
2010-2023
South University
2012
State Street (United States)
2004-2011
University of Notre Dame
2003-2005
Abstract Oligoarray analysis was used to determine the number and nature of genes expressed in third instar Drosophila melanogaster larval midguts. The majority transcripts were associated with protein synthesis metabolism. Serine proteases main proteolytic enzymes detected. Some 40% cytochrome P450 74% glutathione S transferases (GSTs) genome D. observed be midgut by oligoarray analysis. We also identified potential transcription factor binding motifs (TFBMs) P450s, GSTs carboxylesterases....
Anopheles funestus is a primary vector of malaria in Africa south the Sahara. We assessed its rangewide population genetic structure based on samples from 11 countries, using 10 physically mapped microsatellite loci, two per autosome arm and X (N = 548), 834 bp mitochondrial ND5 gene 470). On basis allele frequencies, we found three subdivisions: eastern (coastal Tanzania, Malawi, Mozambique Madagascar), western (Burkina Faso, Mali, Nigeria Kenya), central (Gabon, coastal Angola). A....
Plant-feeding insects have been recently found to use microbes manipulate host plant physiology and morphology. Gall midges are one of the largest groups that plants extensively. Hessian fly (HF, Mayetiola destructor) is an important pest wheat a model system for studying gall midges. To examine role bacteria in parasitism, systematic analysis associated with HF was performed first time. Diverse were different developmental stages. Fluorescent situ hybridization detected bacteriocyte-like...
Abstract An oligoarray analysis was conducted to determine the differential expression of genes due phenobarbital exposure in Drosophila melanogaster ( w 1118 strain) third instar larvae. Seventeen were observed be induced with increased by a statistical microarrays approach q ≤ 0.05. At 0.12, four more Cyp12d1, DmGstd4 , and two unknown function) found up‐regulated, 11 function down‐regulated. Fifteen these genes, Cyp4d14, Cyp6a2 Cyp6a8 Cyp12d1 Cyp6d5 Cyp6w1 CG2065, DmGstd6, DmGstd7...
Journal Article Virulence in Hessian Fly (Diptera: Cecidomyiidae) Field Collections From the Southeastern United States to 21 Resistance Genes Wheat Get access Sue E. Cambron, Cambron Search for other works by this author on: Oxford Academic PubMed Google Scholar G. David Buntin, Buntin Randy Weisz, Weisz Jeffery D. Holland, Holland Kathy L. Flanders, Flanders Brandon J. Schemerhorn, Schemerhorn Richard H. Shukle 6 6Corresponding author, e-mail: rich.shukle@ars.usda.gov of Economic...
Abstract The Hessian fly, Mayetiola destructor (Say), is one of the most important insect pest plaguing wheat (Triticum aestivum, L) producers across United States and around world. Genetic resistance stalwart for control fly. However, new genotypes (biotypes) arise in deployment containing genes, so field populations must be evaluated periodically to provide information on efficacy those deployed genes. Louisiana (LA), with its diverse agricultural landscape, not exempt from challenges...
Abstract Anopheles funestus Giles is one of the most important vectors malaria in sub‐Saharan Africa. The population structure this mosquito Burkina Faso, West Africa based on chromosomal inversion data led to description two forms, Kiribina and Folonzo. Because both forms co‐occur same locales yet differ significantly, frequency inverted arrangements chromosome arms 3R 2R vectorial capacity, they were hypothesized be emerging species with at least partial barriers gene flow. This hypothesis...
Abstract One function of plant lectins such as wheat germ agglutinin is to serve defences against herbivorous insects. The midgut one critical site affected by dietary lectins. We observed marked cellular, structural and gene expression changes in the midguts Drosophila melanogaster third instar larvae that were fed agglutinin. Some these similar those starved D. . Dietary caused shortening, branching, swelling, distortion some cases disintegration microvilli. Starvation was accompanied...
Two nonoverlapping autosomal inversions defined unusual neo-sex chromosomes in the Hessian fly (Mayetiola destructor). Like other chromosomes, these were normally heterozygous, present only one sex, and suppressed recombination around a sex-determining master switch. Their properties originated from anomalous sex determination system which postzygotic chromosome elimination is used to establish karyotypes. This permitted evolution of switch (Chromosome maintenance, Cm) that acts maternally....
The Hessian fly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae), is the most important insect pest of wheat ( Triticum aestivum L. subsp. ) in southeastern United States, and deployment genetically resistant effective control. However, use results selection genotypes that can overcome formerly wheat. We have evaluated effectiveness 16 resistance genes for protection from fly infestation States. Results documented while 10 could provide wheat, highly were H12 , H18 H24 H25 H26 H33 . been...
Abstract We report the water balance of aestivating (summer), diapausing (winter), and non‐diapausing pupae Hessian fly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae). Maintaining requirements during pupal dormancy is particularly important because cannot be replenished actively by drinking. Dehydration tolerance (25% loss before succumbing to dehydration) content (63–65%) were not different for three types pupae. Differences noted in net transpiration rates (NTRs, % body per hour at...
Abstract Background The Hessian fly ( Mayetiola destructor ) is an important insect pest of wheat. It has tractable genetics, polytene chromosomes, and a small genome (158 Mb). Investigation the presents excellent opportunities to study plant-insect interactions molecular mechanisms underlying imprinting chromosome elimination. A physical map needed improve ability perform both positional cloning comparative genomic analyses with fully sequenced genomes other dipteran species. Results An...
Abstract The Hessian fly is a destructive pest of wheat causing severe economic damage. Numerous genes and associated biological pathways have been implicated in defense against fly. However, due to limited genetic resources, compounded with genome complexity, functional analysis the candidate are challenging wheat. Physically, Brachypodium distachyon (Bd) exhibits nonhost resistance fly, small size, short life cycle, vast resources amenability transformation, it offers an alternate genomic...
The Hessian fly (Mayetiola destructor), belonging to the gall midge family (Cecidomyiidae), is a devastating pest of wheat (Triticum aestivum) causing significant yield losses. Despite identification and characterization numerous fly-responsive genes associated biological pathways involved in defense against this dipteran pest, their functional validation has been challenging. This largely attributed large genome, polyploidy, repetitive DNA, limited genetic resources hexaploid wheat. diploid...
Bowman-Birk inhibitor (BBI) is toxic when fed to certain insects, including the fruit fly, Drosophila melanogaster. Dietary BBI has been demonstrated slow growth and increase insect mortality by inhibiting digestive enzymes trypsin chymotrypsin, resulting in a reduced supply of amino acids. In mammals, influences cellular energy metabolism. Therefore, we tested hypothesis that dietary affects energy-associated pathways D. melanogaster midgut. Through microarray metabolomic analyses, show...
Population structure dictates the evolution of each population, and thus, species as a whole. Incorporating spatial variables with population genetic statistics allows for greater discovery beyond traditional genetics alone can inform management decisions. The understanding in Hessian fly, Mayetiola destructor (Say), pest wheat, has been limited past. We scored 14 microsatellite loci from 12 collections fly southeastern United States. Through Bayesian clustering analysis, we found two major...
Domain III of the mitochondrial 12S rRNA gene from Hessian fly, Mayetiola destructor (Say), was sequenced in 21 populations United States, two Canada, five Mediterranean basin, one population Southwest Asia, and New Zealand. From total alignment, seven unique DNA (mtDNA) sequences (haplotypes) were identified. Of haplotypes only three (haplotypes 1, 2, 3) occurred North America, indicating these most likely introduced haplotypes. Haplotypes not restricted to any given biotype M. as defined...
Abstract Evidence is emerging that some proteins secreted by gall‐forming parasites of plants act as effectors responsible for systemic changes in the host plant, such galling and nutrient tissue formation. A large number salivary gland ( SSGP s) are putative physiological elicited susceptible seedling wheat H essian fly, M ayetiola destructor S ay), larvae have been documented. However, how genes encoding these candidate might respond under field conditions unknown. The goal this study was...