A5035676212- Insect Resistance and Genetics
- Mosquito-borne diseases and control
- Reproductive System and Pregnancy
- Insect symbiosis and bacterial influences
- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- Insect-Plant Interactions and Control
- Birth, Development, and Health
- Genomics and Phylogenetic Studies
- Pregnancy and preeclampsia studies
- Genetic and phenotypic traits in livestock
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- Viral Infectious Diseases and Gene Expression in Insects
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Neurobiology and Insect Physiology Research
- Endometriosis Research and Treatment
- MicroRNA in disease regulation
- Insect and Pesticide Research
- Plant Virus Research Studies
- Cancer-related molecular mechanisms research
- Animal Genetics and Reproduction
- RNA modifications and cancer
University of Missouri
2016-2025
University of Missouri Health System
2020
University of Notre Dame
2010-2019
Institute of Animal Sciences
2018-2019
The Graduate Center, CUNY
2016
University of Illinois Urbana-Champaign
2006-2010
Purdue University West Lafayette
2004-2007
Cornell University
2006
Texas A&M University
2006
International Centre for Genetic Engineering and Biotechnology
2000-2001
Parasitoid Wasp Genomes wasps, which prey on and reproduce in host insect species, play important roles plant herbivore interactions, may provide valuable tools the biological control of pest species. The Nasonia Genome Working Group (p. 343 ; see news story by Pennisi ) presents genome three very closely related species: vitripennis, N. giraulti , longicornis . findings document rapid evolution between a endosymbiont that can cause nuclear-cytoplasmic incompatibilities affect speciation.
We characterized Apis mellifera in both native and introduced ranges using 1136 single-nucleotide polymorphisms genotyped 341 individuals. Our results indicate that A. originated Africa expanded into Eurasia at least twice, resulting populations eastern western Europe are geographically close but genetically distant. A third expansion the New World has involved near-replacement of previously "European" honey bees by descendants more recently m. scutellata ("African" or "killer" bees)....
The human endometrium is essential in providing the site for implantation and maintaining growth survival of conceptus. An unreceptive disrupted maternal−conceptus interactions can cause infertility due to pregnancy loss or later complications. Despite this, role uterine glands first trimester little understood. established organoid protocol was used generate comprehensively analyze 3-dimensional endometrial epithelial (EEO) cultures from biopsies. derived EEO expand long-term, are...
Abstract Uterine glands are essential for pregnancy establishment. By employing forkhead box A2 (FOXA2)-deficient mouse models coupled with leukemia inhibitory factor (LIF) repletion, we reveal definitive roles of uterine in embryo implantation and stromal cell decidualization. Here report that LIF from the initiates embryo-uterine communication, leading to attachment Detailed histological molecular analyses discovered crypt formation does not involve glands, but removal luminal epithelium...
Background Codon bias is a phenomenon of non-uniform usage codons whereas codon context generally refers to sequential pair in gene. Although genome sequencing multiple species dipteran and hymenopteran insects have been completed only few these analyzed for bias. Methods Principal Findings Here, we use bioinformatics approaches analyze patterns genome-wide manner among 15 7 insect species. Results show that GAA the most frequent GAG Data reveals ending with C or G are frequently used...
Background The mosquito Aedes aegypti is the primary vector of dengue virus (DENV) infection in humans, and DENV most important arbovirus across subtropics tropics worldwide. early time periods after with define critical cellular processes that determine ultimate success or failure to establish mosquito. Methods Results To identify genes involved these processes, we performed genome-wide transcriptome profiling between susceptible refractory A. strains at two challenging them DENV. Genes...
A major unresolved issue is how the uterus influences infertility and subfertility in cattle. Serial embryo transfer was previously used to classify heifers as high-fertile (HF), subfertile (SF), or infertile (IF). To assess pregnancy loss, two vivo-produced embryos were transferred into HF, SF, IF on day 7, outcome assessed 17. Pregnancy rate substantially higher HF (71%) SF (90%) than (20%) heifers. Elongating conceptuses about twofold longer Transcriptional profiling detected relatively...
The hive-living honeybees (Apis mellifera) show age-dependent behavioural changes; young bees usually nurse the broods in colony and older engage foraging activities. These developmentally regulated changes were previously shown to be correlated with genome-wide transcriptional honeybee brain. indigenous small regulatory RNA molecules, known as microRNAs (miRNAs), are potent regulators of gene expression also regulated. Thus, we wanted study if there might correlation differential miRNA...
Establishment of pregnancy in cattle is complex and encompasses ovulation, fertilization, blastocyst formation growth into an elongated conceptus, recognition signaling, development the embryo placenta. The objective here was to investigate sire influences on establishment cattle. First, 10 Holstein bulls were classified as high or low fertility based their conception rate (SCR) value. In a field trial, at first timed insemination not different between SCR bulls. Next, 5 sires phenotyped...
Abstract Uterine glands are essential for pregnancy in mice and likely humans, because they secrete or transport bioactive substances that regulate uterine receptivity blastocyst implantation. In mice, the uterus becomes receptive to implantation on day 4, but is refractory by 5. Here, blastocysts could be recovered from progesterone-induced gland (PUGKO) not wildtype (WT) 5 post-mating. Anti-adhesive Muc1 protein microvilli were present luminal epithelium of PUGKO WT uteri. A number known...
Progesterone (P4) acts via the endometrium to promote conceptus growth and implantation for pregnancy establishment. Many cells release extracellular vesicles (EVs) that are membrane-bound of endosomal plasma membrane origin. In sheep, endometrial-derived EVs were found traffic trophectoderm. Thus, hypothesized be an important mode intercellular communication by transferring select RNAs, proteins, lipids between conceptus. Electron microscopy analysis endometrial luminal glandular epithelia...
This study investigated bovine conceptus-induced modifications to the endometrial transcriptome related effects of interferon tau (IFNT), conceptus origin (in vivo vs. in vitro), and sex. In vitro (IVF) or (superovulation artificial insemination, AI) produced blastocysts were transferred into recipient heifers on day 7 estrous cycle. On 15, IVF- AI-derived conceptuses obtained by uterine flushing individually placed explants media for 6 h. Explants also cultured with alone as a control...
Arthropod-borne pathogens account for millions of deaths each year. Understanding the genetic mechanisms controlling vector susceptibility to has profound implications developing novel strategies insect-transmitted infectious diseases. The fact that many viruses carry genes have anti-apoptotic activity long led hypothesis induction apoptosis could be a fundamental innate immune response. However, cellular mediating following viral infection remained enigmatic, which prevented experimental...
Abstract The proposed signal for maternal recognition of pregnancy in pigs is estrogen (E2), produced by the elongating conceptuses between days 11 to 12 with a more sustained increase during conceptus attachment and placental development on 15 30. To understand role E2 porcine elongation establishment, loss-of-function study was conducted editing aromatase (CYP19A1) using CRISPR/Cas9 technology. Wild-type (CYP19A1+/+) (CYP19A1−/−) fibroblast cells were used create embryos through somatic...
At least 0.08% of the Apis mellifera nuclear genome contains sequences that originated from mitochondria. These copies mitochondrial (numts) are scattered all over honeybee chromosomes and have by multiple independent insertions DNA (mtDNA) as evident phylogenetic analysis. Apart original insertions, moderate duplications numts also contributed to present pattern distribution in chromosomes. Assimilation genes is mediated extensive fragmentations inserts. Replication slippage seems be a...
Aedes aegypti is the primary mosquito vector for dengue virus (DENV) worldwide. Infectivity of varies among natural populations this mosquito. How A. responds to DENV infection relative which genes and associated pathways contribute its differential susceptibility as a not well defined.Here, we used custom cDNA microarrays identify groups that were differentially expressed in midgut tissues between susceptible refractory strains highly time specific manner. While involved protein processing...
The mosquito Aedes aegypti is the primary vector for medically important arthropod-borne viruses, including chikungunya virus (CHIKV). Following oral acquisition, an arbovirus has to persistently infect several organs in before becoming transmissible another vertebrate host. A major obstacle overcome during its infection cycle inside midgut escape barrier, representing exit mechanism arboviruses utilize when disseminating from midgut. To understand transcriptomic basis of and reveal genes...
Infertility and subfertility represent major problems in domestic animals humans, the majority of embryonic loss occurs during first month gestation that involves pregnancy recognition conceptus implantation. The critical genes physiological pathways endometrium mediate establishment success are not well understood. In study one, predominantly Angus heifers were classified based on fertility using serial embryo transfer to select with intrinsic differences loss. each four rounds, a single...