A5050988587- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Reproductive System and Pregnancy
- Sperm and Testicular Function
- Genetic Mapping and Diversity in Plants and Animals
- Cancer-related molecular mechanisms research
- Effects of Environmental Stressors on Livestock
- Pregnancy and preeclampsia studies
- Prenatal Screening and Diagnostics
- Endometriosis Research and Treatment
- Renal and related cancers
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Assisted Reproductive Technology and Twin Pregnancy
- Pluripotent Stem Cells Research
- Kruppel-like factors research
- Coenzyme Q10 studies and effects
- Autophagy in Disease and Therapy
- Mesenchymal stem cell research
- Livestock Management and Performance Improvement
- Urological Disorders and Treatments
- Agriculture Sustainability and Environmental Impact
- Single-cell and spatial transcriptomics
University of Wisconsin–Madison
2022-2025
University of Missouri
2018-2023
Recombinetics (United States)
2019
University of Florida
2013-2019
University of California, Santa Cruz
2017
Florida Department of Agriculture and Consumer Services
2015
Universidad Juárez del Estado de Durango
1984
Successful embryonic development is dependent on factors secreted by the reproductive tract. Dickkopf-1 (DKK1), an antagonist of wingless-related mouse mammary tumor virus (WNT) signaling pathway, one endometrial secretory protein potentially involved in maternal-embryo communication. The purpose this study was to investigate roles DKK1 embryo cell fate decisions and competence establish pregnancy. Using vitro-produced bovine embryos, we demonstrate that exposure embryos during period morula...
There is species divergence in control of DNA methylation during preimplantation development. The exact pattern the bovine embryo has not been established nor its regulation by gender or maternal signals that regulate development such as colony stimulating factor 2 (CSF2). Using immunofluorescent labeling with anti-5-methylcytosine and embryos produced X-chromosome sorted sperm, it was demonstrated decreased from 2-cell stage to 6–8 cell then increased thereafter up blastocyst stage. In a...
Summary We evaluated 69 SNP s in genes previously related to fertility and production traits for their relationship daughter pregnancy rate ( DPR ), cow conception CCR ) heifer HCR a separate population of Holstein cows grouped according predicted transmitting ability (PTA) [≤−1 n = 1287) ≥1.5 1036)] . Genotyping was performed using Sequenom Mass ARRAY ® There were total 39 associated with the three traits. The that explained greater proportion genetic variation COQ 9 (3.2%), EPAS 1 (1.0%),...
Establishment of pregnancy in cattle is complex and encompasses ovulation, fertilization, blastocyst formation growth into an elongated conceptus, recognition signaling, development the embryo placenta. The objective here was to investigate sire influences on establishment cattle. First, 10 Holstein bulls were classified as high or low fertility based their conception rate (SCR) value. In a field trial, at first timed insemination not different between SCR bulls. Next, 5 sires phenotyped...
In vitro embryo production systems are limited by their inability to consistently produce embryos with the competency develop blastocyst stage, survive cryopreservation, and establish a pregnancy. Previous work identified combination of three cytokines [fibroblast growth factor 2 (FGF2), leukemia inhibitory (LIF), insulin-like 1 (IGF1)], called FLI, that we hypothesize improve preimplantation development bovine in vitro. To test this hypothesis, FLI was supplemented into oocyte maturation or...
Ruminants have a semi-invasive placenta, which possess highly vascularized placentomes formed by maternal endometrial caruncles and fetal placental cotyledons required for development to term. The synepitheliochorial placenta of cattle contains at least two trophoblast cell populations, including uninucleate (UNC) binucleate (BNC) cells that are most abundant in the cotyledonary chorion placentomes. interplacentomal is more epitheliochorial nature with developing specialized areolae over...
Infertility and subfertility represent major problems in domestic animals humans, the majority of embryonic loss occurs during first month gestation that involves pregnancy recognition conceptus implantation. The critical genes physiological pathways endometrium mediate establishment success are not well understood. In study one, predominantly Angus heifers were classified based on fertility using serial embryo transfer to select with intrinsic differences loss. each four rounds, a single...
Summary Dairy cows with increased rectal temperature experience lower milk yield and fertility. Rectal during heat stress is heritable, so genetic selection for body regulation could reduce effects of on production. One aim the study was to validate relationship between genotype tolerance single nucleotide polymorphisms ( SNP s) previously associated resistance stress. A second identify new s resistance. Thermotolerance assessed in lactating Holsteins summer by measuring (a direct...
Abstract During preimplantation development, the embryo undergoes two consecutive lineages specifications. The first cell fate decision determines which cells give rise to trophectoderm (TE) and inner mass (ICM). Subsequently, ICM differentiates into hypoblast epiblast, latter giving proper. transcription factors that govern these decisions have been extensively studied in mouse, but are still poorly understood other mammalian species. In present study, role of NANOG formation epiblast...
A single missense mutation at position 159 of coenzyme Q9 (COQ9) (G→A; rs109301586) has been associated with genetic variation in fertility Holstein cattle, the allele higher fertility. COQ9 is involved synthesis COQ10, a component electron transport system mitochondria. Here we tested whether reproductive phenotype and evaluated functional consequences for cellular oxygen metabolism, body weight changes, ovarian function. The modifies predicted tertiary protein structure affected...
SUMMARY The microenvironment of a preimplantation embryo can influence changes in development that affect postnatal phenotypes. One the potential mediators this effect many species is colony‐stimulating factor (CSF2), which increase an embryo's ability to establish pregnancy after its transfer into recipients. Exposure embryos CSF2 during early also pattern later sex‐dependent manner. We therefore hypothesized treatment vitro‐produced with culture would alter birth weight and growth...
Colony-stimulating factor 2 (CSF2) is an embryokine that improves competence of the embryo to establish pregnancy and which may participate in developmental programming. We tested whether culture bovine embryos with CSF2 alters fetal development alleviates abnormalities associated vitro production (IVP) embryos. Pregnancies were established by artificial insemination (AI), transfer IVP (IVP), or treated 10 ng/ml from day 5 7 (CSF2). produced using X-sorted semen. Female singleton conceptuses...
The hypothesis was that early postpartum uterine disease would reduce the developmental capacity of oocytes thus contributing to reduced fertility dairy cows with disease. Dairy were diagnosed healthy or metritis at 7 10 d postpartum. reproductive tract collected approximately 1 mo (Exp. 1) 80 165 2) for collection cumulus-oocyte complexes (COC). COC matured, co- incubated sperm fertilization, and cultured blastocyst stage (8 d) in vitro. For Exp.1, diagnosis (healthy metritis) did not...
Abstract A central determinant of successful reproduction is pregnancy establishment and maintenance that relies on proper development the conceptus (embryo/fetus associated extraembryonic membranes including placenta). Pregnancy loss in cattle can be caused by inadequate differentiation placenta. However, cellular molecular mechanisms regulating bovine placenta and, particularly, trophoblast are not well understood. Recent single-cell RNA-seq analyses revealed dynamic changes cell...
The goal was to gain understanding of how 12 genes containing SNP previously related embryo competence become a blastocyst (BRINP3, C1QB, HSPA1L, IRF9, MON1B, PARM1, PCCB, PMM2, SLC18A2, TBC1D24, TTLL3 and WBP1) participate in embryonic development. Gene expression evaluated matured oocytes embryos. BRINP3 C1QB were not detected at any stage. For most other genes, transcript abundance declined as the developed Exceptions for PARM1 WBP1, where steady-state mRNA increased 9–16 cell WBP1 caused...
The first week of gestation is a period major pregnancy loss in cattle, this study reveals that the male plays key role regulating embryonic development during time.The impact sire on preimplantation cattle remains poorly understood. This evaluated differences embryos produced vitro from sires with varying capacities to produce blastocysts. Sires classified as high (HP) and low performing (LP) based their ability were used better understand how regulates development. By monitoring...