Rebecca Lee
A5100708422- Cancer Genomics and Diagnostics
- CAR-T cell therapy research
- Melanoma and MAPK Pathways
- Cutaneous Melanoma Detection and Management
- COVID-19 and healthcare impacts
- Cancer Immunotherapy and Biomarkers
- Legal principles and applications
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- COVID-19 Clinical Research Studies
- Blood Pressure and Hypertension Studies
- Heart Rate Variability and Autonomic Control
- Childhood Cancer Survivors' Quality of Life
- Adolescent and Pediatric Healthcare
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Mast cells and histamine
- Schizophrenia research and treatment
- Healthcare cost, quality, practices
- Corporate Governance and Law
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Colorectal Cancer Treatments and Studies
- Receptor Mechanisms and Signaling
- Diversity and Career in Medicine
- Health Policy Implementation Science
The Christie NHS Foundation Trust
2013-2025
University of Manchester
2015-2025
Natural Resources Canada
2023-2025
Keck Hospital of USC
2025
University of Southern California
2017-2025
Geological Survey of Canada
2024-2025
Baylor University Medical Center
2024-2025
Cancer Research UK Manchester Institute
2015-2024
Centre for Mental Health
2024
The University of Melbourne
2024
Folic acid was covalently conjugated to 66-nm liposomes via spacers of various lengths in an attempt target the KB cells expressing folate receptors. Spacers short and intermediate were unable mediate association folate-conjugated with receptor-bearing cells, however, use a 250 A polyethyleneglycol spacer (PEG, M(r) approximately 3350) permitted avid uptake at 2.5 x 10(5) sites/cell. The binding folate-PEG could be competitively inhibited by excess free or antiserum against receptor,...
Raised blood pressure (BP) is the biggest contributor to mortality and disease burden worldwide fewer than half of those with hypertension are aware it. May Measurement Month (MMM) a global campaign set up in 2017, raise awareness high BP as pragmatic solution lack formal screening worldwide. The 2018 was expanded, aiming include more participants countries.
Targeted therapies and immunotherapies have transformed melanoma care, extending median survival from ∼9 to over 25 months, but nevertheless most patients still die of their disease. The aim precision medicine is tailor care for individual improve outcomes. To this end, we developed protocols facilitate individualized treatment decisions with advanced melanoma, analyzing 364 samples 214 patients. Whole exome sequencing (WES) targeted circulating tumor DNA (ctDNA) allowed us monitor responses...
The advent of effective adjuvant therapies for patients with resected melanoma has highlighted the need to stratify based on risk relapse given cost and toxicities associated treatment. Here we assessed circulating tumor DNA (ctDNA) predict monitor in stage III melanoma.
This paper contributes with a pragmatic evaluation framework for explainable Machine Learning (ML) models clinical decision support. The study revealed more nuanced role ML explanation models, when these are pragmatically embedded in the context. Despite general positive attitude of healthcare professionals (HCPs) towards explanations as safety and trust mechanism, significant set participants there were negative effects associated confirmation bias, accentuating model over-reliance...
Recent sham-controlled randomized clinical trials have confirmed the safety and efficacy of catheter-based renal denervation (RDN). Long-term data beyond 3 years are scarce. Here, we report on outcomes after RDN in a cohort patients with resistant hypertension an average ≈9-year follow-up (FU).We recruited who were previously enrolled various applying radiofrequency energy for blood pressure (BP) lowering. All participants had baseline assessments before repeat assessment at long-term FU...
Abstract Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, calmodulin. Two overlapping clones, isolated from chicken oviduct cDNA plasmid library gizzard lambda NM1149 library, were used to generate 4108-base pair sequence coding for one caldesmon. Expression of the confirms this large caldesmons. The deduced molecular weight 86.974, significantly less than weights estimated by sodium dodecyl sulfate gel electrophoresis. has high...
Abstract It would be much easier to assess the effectiveness of different reintroduction methods, and so improve success reintroductions, if there was greater standardization in documentation methods outcomes. We suggest a series standards for documenting monitoring outcomes associated with projects birds. Key suggestions are: planned release before it occurs, specifying information required on each release, postrelease occurring at standard intervals 1 5 years (and 10 long‐lived species),...
IntroductionTumour burden is a prognostic biomarker in metastatic melanoma. However, tumour difficult to measure and there are currently no reliable surrogate biomarkers easily reliably determine it. The aim of this study was assess the potential plasma total cell free DNA as prognosis melanoma patients.Materials methodsA prospective cohort for circulating cell-free (cfDNA) concentration performed 43 patients. For 38 patients, paired blood collections scan assessments were available before...
Patients with high-risk stage II/III resected melanoma commonly develop distant metastases. At present, we cannot differentiate between patients who will recur or those are cured by surgery. We investigated if circulating tumor DNA (ctDNA) can predict relapse and survival in melanoma.
Despite substantial clinical benefit of targeted and immune checkpoint blockade-based therapies in melanoma, resistance inevitably develops. We show cytoskeletal remodeling changes expression activity ROCK-myosin II pathway during acquisition to MAPK inhibitors. regulates myosin activity, but after initial therapy response, drug-resistant clones restore increase survival. High correlates with aggressiveness, identifying therapy- immunotherapy-resistant melanomas. Survival resistant cells is...
Background Aedes albopictus is an important dengue vector because of its aggressive biting behavior and rapid spread out native home range in Southeast Asia. Pyrethroids are widely used for adult mosquito control, resistance to pyrethroids should be carefully monitored control the only effective method currently available prevent transmission. The voltage-gated sodium channel gene target site pyrethroids, mutations this cause knockdown (kdr). Previous studies reported various (VGSC) gene,...
Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) complex and autophagy-specific class III phosphatidylinositol 3-kinase I (PtdIns3K-C1). The latter contains PIK3C3/VPS34, PIK3R4/VPS15, BECN1/Beclin 1 ATG14 phosphorylates to generate 3-phosphate (PtdIns3P). Here, we show that PIK3C3, BECN1 contain functional LIR motifs...
The cysteine protease ATG4B cleaves off one or more C-terminal residues of the inactive proform proteins ortholog and paralog LC3 GABARAP subfamilies yeast Atg8 to expose a glycine that is conjugated phosphatidylethanolamine during autophagosome formation. We show contains LC3-interacting region (LIR) motif important for efficient binding cleavage proteins. solved crystal structures GABARAPL1-ATG4B LIR complex. Analyses in vitro assays, using specific point mutants, clearly showed binds via...
Abstract Whole-brain radiotherapy (WBRT) is the treatment backbone for many patients with brain metastasis; however, its efficacy in preventing disease progression and associated toxicity have questioned clinical impact of this approach emphasized need alternative treatments. Given limited therapeutic options available these poor understanding molecular mechanisms underlying resistance metastatic lesions to WBRT, we sought uncover actionable targets biomarkers that could help refine patient...
Large cohorts of patients with active cancers and COVID-19 infection are needed to provide evidence the association recent cancer treatment type mortality.
Overactivation of renal sympathetic nervous system and low-grade systemic inflammation are common features hypertension. Renal denervation (RDN) reduces activity in patients with resistant However, its effect on has not been examined. We prospectively investigated the RDN monocyte activation uncontrolled hypertension scheduled for RDN. Ambulatory blood pressure, monocyte, subset inflammatory markers were assessed at baseline, 3 months, 6 months after procedure 42 patients. significantly...
Abstract The therapeutic potential of administering stem cells to promote angiogenesis and myocardial tissue regeneration after infarction has recently been demonstrated. Given the advantages using embryonic mouse models for furthering development this approach, purpose study was determine if could be loaded with superparamagnetic iron oxide (SPIO) particles imaged in a model over time MRI. Mouse were labeled SPIO particles. When incubated 11.2, 22.4, 44.8 μg Fe/ml particles, took up...